Deconstruction of the Beaten Path-Sidestep interaction network provides insights into neuromuscular system development
- California Institute of Technology, United States
- Trinity College Dublin, Ireland
- University of Chicago, United States
- Ellipsis Health, United States
- Howard Hughes Medical Institute, Stanford University School of Medicine, United States
Abstract
An "interactome" screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we "deorphanized" four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.
Article and author information
Author details
Siamak K Sorooshyari
K Christopher Garcia
Funding
NIH (R37)
- Kai Zinn
SFI
- Juan-Pablo Labrador
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Graeme W Davis, University of California, San Francisco, United States
Version history
- Received: April 27, 2017
- Accepted: July 28, 2017
- Accepted Manuscript published: August 15, 2017 (version 1)
- Accepted Manuscript updated: August 15, 2017 (version 2)
- Version of Record published: August 31, 2017 (version 3)
Copyright
© 2017, Li et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Deconstruction of the Beaten Path-Sidestep interaction network provides insights into neuromuscular system development
eLife 6:e28111.
https://doi.org/10.7554/eLife.28111
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