Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

  1. Lin Cong
  2. Zeguan Wang
  3. Yuming Chai
  4. Wei Hang
  5. Chunfeng Shang
  6. Wenbin Yang
  7. Lu Bai
  8. Jiulin Du
  9. Kai Wang  Is a corresponding author
  10. Quan Wen  Is a corresponding author
  1. Chinese Academy of Sciences, China
  2. Hefei National Laboratory for Physical Sciences at Microscale, China

Abstract

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

Article and author information

Author details

  1. Lin Cong

    Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  2. Zeguan Wang

    Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Yuming Chai

    Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Wei Hang

    Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Chunfeng Shang

    Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  6. Wenbin Yang

    Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China
    Competing interests
    The authors declare that no competing interests exist.
  7. Lu Bai

    Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  8. Jiulin Du

    Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    Competing interests
    The authors declare that no competing interests exist.
  9. Kai Wang

    Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
    For correspondence
    wangkai@ion.ac.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7858-944X
  10. Quan Wen

    Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China
    For correspondence
    qwen@ustc.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0268-8403

Funding

Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02060012)

  • Kai Wang

National Science Foundation of China (NSFC-31471051)

  • Quan Wen

China Thousand Talents Program

  • Kai Wang

CAS Pioneer Hundred Talents Program

  • Quan Wen

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Zebrafish handling procedures were approved by the Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.(permit number: USTCACUC1103013).

Reviewing Editor

  1. Ronald L Calabrese, Emory University, United States

Publication history

  1. Received: April 28, 2017
  2. Accepted: September 11, 2017
  3. Accepted Manuscript published: September 20, 2017 (version 1)
  4. Version of Record published: October 17, 2017 (version 2)

Copyright

© 2017, Cong et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Lin Cong
  2. Zeguan Wang
  3. Yuming Chai
  4. Wei Hang
  5. Chunfeng Shang
  6. Wenbin Yang
  7. Lu Bai
  8. Jiulin Du
  9. Kai Wang
  10. Quan Wen
(2017)
Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
eLife 6:e28158.
https://doi.org/10.7554/eLife.28158

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