Abstract
The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.
Article and author information
Author details
Funding
Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02060012)
- Kai Wang
National Science Foundation of China (NSFC-31471051)
- Quan Wen
China Thousand Talents Program
- Kai Wang
CAS Pioneer Hundred Talents Program
- Quan Wen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Ronald L Calabrese, Emory University, United States
Ethics
Animal experimentation: Zebrafish handling procedures were approved by the Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.(permit number: USTCACUC1103013).
Version history
- Received: April 28, 2017
- Accepted: September 11, 2017
- Accepted Manuscript published: September 20, 2017 (version 1)
- Version of Record published: October 17, 2017 (version 2)
Copyright
© 2017, Cong et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
eLife 6:e28158.
https://doi.org/10.7554/eLife.28158
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