Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

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Abstract

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

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Lin Cong

Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Zeguan Wang

Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China

Competing interests
The authors declare that no competing interests exist.
Yuming Chai

Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China

Competing interests
The authors declare that no competing interests exist.
Wei Hang

Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Chunfeng Shang

Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Wenbin Yang

Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China

Competing interests
The authors declare that no competing interests exist.
Lu Bai

Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Jiulin Du

Insitute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Kai Wang

Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China

For correspondence
wangkai@ion.ac.cn

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-7858-944X
Quan Wen

Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, China

For correspondence
qwen@ustc.edu.cn

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-0268-8403

Funding

Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02060012)

Kai Wang

National Science Foundation of China (NSFC-31471051)

Quan Wen

China Thousand Talents Program

Kai Wang

CAS Pioneer Hundred Talents Program

Quan Wen

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Zebrafish handling procedures were approved by the Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.(permit number: USTCACUC1103013).

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.