Essential role for SUN5 in anchoring sperm head to the tail
- Institute of Zoology, Chinese Academy of Sciences, China
- University of Chinese Academy of Sciences, China
- The First Affiliated Hospital of Anhui Medical University, China
- Anhui Medical University, China
- Nanjing Medical University, China
Figures
Figure 1 with 3 supplements
Ablation of SUN5 leads to male infertility and sperm malformation.
(A) Immunoblotting of SUN5 in WT, Sun5+/− and Sun5−/− testes. (B) The size of the testes was not altered in the Sun5+/−and Sun5−/− mice. (C) The pregnancy rate of WT (92.46 ± 3.39%), Sun5+/− (88.33 …
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Figure 1—source data 1
Source data for mouse fertility, sperm concentration, sperm motility and spermatozoa components in epididymis.
- https://doi.org/10.7554/eLife.28199.006
Figure 1—figure supplement 1
The generation of Sun5 knockout mice.
(A) The expression of Sun5 was restricted to testis. (B) Sun5 expression begins in 3-week-old testes. (C) The knockout strategy of Sun5 in mice. (D) Genotyping of founders to identify Sun5 …
Figure 1—figure supplement 2
Sun5 knockout does not affect growth and testis development, but influences sperm motility.
(A) Body weight of WT, Sun5+/− and Sun5−/− male mice, showing that Sun5 knockout does not affect mouse growth (n = 5). (B) Testis weight of adult WT, Sun5+/− and Sun5−/− male mice, indicating that …
Figure 1—figure supplement 3
All of the biallelic Sun5 mutated male mice exhibit similar defects in spermiogenesis.
(A) All of the biallelic Sun5 mutated males showed normal testis structures but had abnormal HE staining in the caudal epididymis. Seminiferous tubules shown in the figures were at stage IV-VI. …
Figure 2 with 2 supplements
The absence of SUN5 has no effect on acrosome biogenesis but disrupts the development of the coupling apparatus between sperm head and tail.
(A) IF (immunofluorescence) staining of sp56 in WT and Sun5-null spermatozoon. The Sun5-null spermatozoa contains both round-headed spermatozoa and tailless heads (lower two panels). The proportion …
Figure 2—figure supplement 1
Acrosome biogenesis and epididymal spermatozoa in Sun5−/− testes.
(A) Immunohistochemistry staining of Afaf in WT and Sun5−/− testes, indicating that acrosome biogenesis was not affected in Sun5−/− testes. Scale bar: 10 μm. (B) sp56 IF staining in Sun5–null …
Figure 2—figure supplement 2
Spermiation defects in Sun5−/− mice.
(A) Periodic acid-Schiff (PAS) staining revealed histology of all twelve developmental stages in WT and Sun5−/− testes. The morphology of Acrosome (Ac) marks the specific developmental stages. Scale …
Figure 3
SUN5 localizes to the coupling apparatus between the sperm head and tail in mammals.
(A) IF of SUN5 in testes. Scale bar: upper panel, 10 μm; lower panel, 2.5 μm. (B) IF of SUN5 in spermatids at different developmental stages. Scale bar: 5 μm. (C) Single-sperm immunofluorescence of …
Figure 4 with 1 supplement
Infertility caused by SUN5 mutations could be overcome by ICSI.
(A) Representative images and (B) genotypes of the Sun5-null ICSI offspring. (C) Representative images and (D) genotypes of the WT ICSI offspring. (E) Pedigree of family 1 with inherited SUN5 …
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Figure 4—source data 1
The sperm motility and morphology analysis of the two patients underwent ICSI.
- https://doi.org/10.7554/eLife.28199.013
Figure 4—figure supplement 1
Development of WT and Sun5-null ICSI offspring.
(A) Body weight of WT and Sun5−/− ICSI offspring, statistical analysis was performed using at least three individuals. (B) Testis weight of adult WT and Sun5−/− ICSI offspring, statistical analysis …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.28199.014
