The human cytoplasmic dynein interactome reveals novel activators of motility
- University of California, San Diego, United States
- Northwestern University, United States
- Stowers Institute of Medical Research, United States
- Stowers Institute for Medical Research, United States
Abstract
In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome by attaching a promiscuous biotin ligase ("BioID") to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. This method reported spatial information about the large cytosolic dynein/dynactin complex in living cells. To achieve maximal motile activity and to bind its cargos, human dynein/dynactin requires "activators", of which only five have been described. We developed methods to identify new activators in our BioID data, and discovered that ninein and ninein-like are a new family of dynein activators. Analysis of the protein interactomes for six activators, including ninein and ninein-like, suggests that each dynein activator has multiple cargos.
Article and author information
Author details
Michael P Washburn
Samara L Reck-Peterson
Funding
National Institutes of Health (R01GM121772)
- Willam B Redwine
- Phuoc Tien Tran
- Samara L Reck-Peterson
Howard Hughes Medical Institute (Faculty Scholar)
- Morgan E DeSantis
- Samara L Reck-Peterson
Simons Foundation (Faculty Scholar)
- Morgan E DeSantis
- Samara L Reck-Peterson
National Institutes of Health (R01GM107214)
- Ian Hollyer
- Samara L Reck-Peterson
Jane Coffin Childs Memorial Fund for Medical Research
- Morgan E DeSantis
National Science Foundation
- Zaw Min Htet
Stowers Institute for Medical Research
- Selene K Swanson
- Laurence Florens
- Michael P Washburn
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Redwine et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 9,243
- views
-
- 1,454
- downloads
-
- 148
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
The human cytoplasmic dynein interactome reveals novel activators of motility
eLife 6:e28257.
https://doi.org/10.7554/eLife.28257
