(A) Occupancy of the indicated 3x-HA-FRB tagged Mediator subunit at the indicated enhancers prior to (-Rap) or after (+Rap) being depleted by anchor away. The parental strain containing no …
(A) Mean Pol II occupancy over ~ 400 transcribed genes prior to and after anchor-away of Med14, Med17, TBP, or the parental strain (WT); data is the same as shown in Figure 1B. Pol II data for the me…
TFIID-dependent genes. (A) Mean Pol II occupancy for the indicated groups of genes (SAGA or TFIID-dependent) prior to and after anchor-away of Med17, Med14, and Med15, as well as for the parent …
Sequence reads were normalized as counts per million (CPM), and the curves were aligned relative to the transcription start site (TSS).
(A) Overlaid mean Pol II occupancy curves scaled to 100% (maximum levels) after anchor-away of the indicated head and middle subunits of Mediator. (B) Overlaid mean Pol II occupancy curves scaled to …
Sequence reads were normalized as counts per million (CPM), and the curves were aligned relative to the transcription start site (TSS).
(A) Pol II occupancy in the coding regions and Mediator subunits at the promoters of the CCW12 and TEF1 genes, before or after anchor-away of Med17, Kin28, or both simultaneously. (B) TBP and TFIIB …
(A) Occupancy of Pol II in the coding regions, and Mediator (Med8 and Med22 subunits), TBP, and TFIIB subunits at the promoters of the inducible HSP12, HSP82, and CUP1 genes before or after …
Occupancy of Mediator (Med8 and Med22 subunits), TBP, and TFIIB at the promoters (A) SED1 and (B) PMA1 in the presence of absence of rapamycin and the presence or absence of a heat shock at 39°C.
(A) Occupancy of Pol II in the coding regions, and Med22, TBP, and TFIIB at the promoters of the indicated genes before or after simultaneous depletion of TBP and Kin28. (B) Occupancy of Pol II in …
The ratio of S5-phosphorylated CTD levels relative to total Rpb1 levels at the promoters of the indicated genes, depletion of the indicated proteins or the control strain (WT).
(A) Pol II occupancy in the coding regions of the indicated constitutive and inducible genes for the parent strain (WT) and ‘triple’ mutant strain, in which the tail module is inactivated via …
(A) growth on YPD (2.5 days) in the presence or absence rapamycin upon anchor-away mediated depletion of the Mediator subunit (light blue shading) or general transcription factor (dark blue shading) …
List of strains.