1. Biochemistry and Chemical Biology
  2. Structural Biology and Molecular Biophysics

Ligand modulation of sidechain dynamics in a wild-type human GPCR

  1. Lindsay D Clark
  2. Igor Dikiy
  3. Karen Chapman
  4. Karin E J Rödström
  5. James Aramini
  6. Michael V LeVine
  7. George Khelashvili
  8. Søren G F Rasmussen
  9. Kevin H Gardner  Is a corresponding author
  10. Daniel M Rosenbaum  Is a corresponding author
  1. The University of Texas Southwestern Medical Center, United States
  2. CUNY Advanced Science Research Center, United States
  3. University of Copenhagen, Denmark
  4. Weill Cornell Medical College, United States
https://doi.org/10.7554/eLife.28505
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Cite this article
  1. Lindsay D Clark
  2. Igor Dikiy
  3. Karen Chapman
  4. Karin E J Rödström
  5. James Aramini
  6. Michael V LeVine
  7. George Khelashvili
  8. Søren G F Rasmussen
  9. Kevin H Gardner
  10. Daniel M Rosenbaum
(2017)
Ligand modulation of sidechain dynamics in a wild-type human GPCR
eLife 6:e28505.
https://doi.org/10.7554/eLife.28505
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Abstract

GPCRs regulate all aspects of human physiology, and biophysical studies have deepened our understanding of GPCR conformational regulation by different ligands. Yet there is no experimental evidence for how sidechain dynamics control allosteric transitions between GPCR conformations. To address this deficit, we generated samples of a wild-type GPCR (A2AR) that are deuterated apart from 1H/13C NMR probes at isoleucine δ1 methyl groups, which facilitated 1H/13C methyl TROSY NMR measurements with opposing ligands. Our data indicate that low [Na+] is required to allow large agonist-induced structural changes in A2AR, and that patterns of sidechain dynamics substantially differ between agonist (NECA) and inverse agonist (ZM241385) bound receptors, with the inverse agonist suppressing fast ps-ns timescale motions at the G protein binding site. Our approach to GPCR NMR creates a framework for exploring how different regions of a receptor respond to different ligands or signaling proteins through modulation of fast ps-ns sidechain dynamics.

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The following data sets were generated
The following previously published data sets were used

Article and author information

Author details

  1. Lindsay D Clark

    Department of Biophysics, The University of Texas Southwestern Medical Center, Dallas, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6879-6883

  2. Igor Dikiy

    Structural Biology Initiative, CUNY Advanced Science Research Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8155-7788

  3. Karen Chapman

    Department of Biophysics, The University of Texas Southwestern Medical Center, Dallas, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Karin E J Rödström

    Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark
    Competing interests
    The authors declare that no competing interests exist.

  5. James Aramini

    Structural Biology Initiative, CUNY Advanced Science Research Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Michael V LeVine

    Department of Physiology and Biophysics, Weill Cornell Medical College, New York, United States
    Competing interests
    The authors declare that no competing interests exist.

  7. George Khelashvili

    Department of Physiology and Biophysics, Weill Cornell Medical College, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7235-8579

  8. Søren G F Rasmussen

    Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark
    Competing interests
    The authors declare that no competing interests exist.

  9. Kevin H Gardner

    Structural Biology Initiative, CUNY Advanced Science Research Center, New York, United States
    For correspondence
    Kevin.Gardner@asrc.cuny.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8671-2556

  10. Daniel M Rosenbaum

    Department of Biophysics, The University of Texas Southwestern Medical Center, Dallas, United States
    For correspondence
    dan.rosenbaum@utsouthwestern.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7174-4993

Funding

Welch Foundation (I-1770)

  • Daniel M Rosenbaum

National Institutes of Health (R01GM113050)

  • Daniel M Rosenbaum

National Institutes of Health (R01GM106239)

  • Kevin H Gardner

National Science Foundation (1000136529)

  • Lindsay D Clark

American Heart Association (16PRE27200004)

  • Lindsay D Clark

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. William I Weis, Stanford University Medical Center, United States

Version history

  1. Received: May 10, 2017
  2. Accepted: September 20, 2017
  3. Accepted Manuscript published: October 6, 2017 (version 1)
  4. Version of Record published: October 20, 2017 (version 2)

Copyright

© 2017, Clark et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Lindsay D Clark
  2. Igor Dikiy
  3. Karen Chapman
  4. Karin E J Rödström
  5. James Aramini
  6. Michael V LeVine
  7. George Khelashvili
  8. Søren G F Rasmussen
  9. Kevin H Gardner
  10. Daniel M Rosenbaum
(2017)
Ligand modulation of sidechain dynamics in a wild-type human GPCR
eLife 6:e28505.
https://doi.org/10.7554/eLife.28505

Share this article

https://doi.org/10.7554/eLife.28505

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