strain, strain background (S. pombe) | WT | ATCC | | KGY425: h- his3-D1 leu1-32 ura4-D18 ade6-M210 |
strain, strain background (S. pombe) | ofd1Δ | this study | | PEY1801: h- his3-D1 leu1-32 ura4-D18 ade6-M210 ofd1Δ::natMX6 |
strain, strain background (S. pombe) | ofd1 H142A D144A | PMID: 18418381 | | PEY1152: h- his3-D1 leu1-32 ura4-D18 ade6-M210 ofd1 H142A D144A |
strain, strain background (S. pombe) | nro1Δ | this study | | PEY1802: h- his3-D1 leu1-32 ura4-D18 ade6-M210 nro1Δ::natMX6 |
strain, strain background (S. pombe) | rps2302Δ | this study | | PEY1803: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302Δ::natMX6 |
strain, strain background (S. pombe) | rps23Δ; rps23Δ-natr | this study | | PEY1804: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps23Δ::natMX6 |
strain, strain background (S. pombe) | rps2302-GFP | this study | | PEY1805: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302+- GFP(S65T)-kanMX6 |
strain, strain background (S. pombe) | rps2302-GFP nro1Δ | this study | | PEY1806: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302+- GFP(S65T)-kanMX6 nro1Δ::natMX6 |
strain, strain background (S. pombe) | rps2302-GFP ofd1Δ | this study | | PEY1847: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302+- GFP(S65T)-kanMX6 ofd1Δ::natMX6 |
strain, strain background (S. pombe) | rps2302-GFP ofd1 H142A D144A | this study | | PEY1848: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302+- GFP(S65T)-kanMX6 ofd1 H142A D144A |
strain, strain background (S. pombe) | rps2302 P62A-GFP | this study | | PEY1849: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302 P62A-GFP(S65T)- TADH1- PURA4-kanr-TTEF |
strain, strain background (S. pombe) | mCherry-ofd1 | this study | | PEY1807: h- his3-D1 leu1-32 ura4-D18 ade6-M210 mCherry- ofd1-TADH1-PURA4-kanr-TTEF |
strain, strain background (S. pombe) | mCherry-ofd1 rps23Δ | this study | | PEY1808: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps23Δ:: natMX6 mCherry-ofd1-TADH1- PURA4-kanr-TTEF |
strain, strain background (S. pombe) | mCherry-ofd1 nro1Δ | this study | | PEY1809: h- his3-D1 leu1-32 ura4-D18 ade6-M210 nro1Δ::kanMX6 mCherry-ofd1-TADH1- PURA4-kanr-TTEF |
strain, strain background (S. pombe) | rps2302Δ sre1N | this study | | PEY1811: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302Δ:: natMX6 sre1(aa1-440) |
strain, strain background (S. pombe) | rps23Δ sre1N | this study | | PEY1812: h- his3-D1 leu1-32 ura4-D18 ade6-M210 rps23Δ:: natMX6 sre1(aa1-440) |
strain, strain background (S. pombe) | rps23Δ ofd1Δ sre1N | this study | | PEY1813: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 ofd1Δ::kanMX6 rps23Δ:: natMX6 sre1(aa1-440) |
strain, strain background (S. pombe) | rps2302Δ ofd1Δ sre1N | this study | | PEY1814: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 ofd1Δ::kanMX6 rps2302Δ::natMX6 sre1(aa1-440) |
strain, strain background (S. pombe) | sre1Δ | PMID: 15797383 | | PEY522: h- his3-D1 leu1-32 ura4-D18 ade6-M210 sre1Δ::kanMX6 |
strain, strain background (S. pombe) | WT | Bioneer | | ED666: h+ leu1-32 ura4-D18 ade6-M210 |
strain, strain background (S. pombe) | rps23Δ | Bioneer | | h+ ade6A14:E25-M210 or ade6-M216 ura4-D18 leu1-32 rps23Δ::kanMX4 |
strain, strain background (S. pombe) | rpl23Δ | Bioneer | | h+ ade6-M210 or ade6-M216 ura4-D18 leu1-32 rpl23Δ::kanMX4 |
strain, strain background (S. pombe) | sre1Δ | Bioneer | | h+ ade6-M210 or ade6-M216 ura4-D18 leu1-32 sre1Δ::kanMX4 |
strain, strain background (S. pombe) | rps25Δ | Bioneer | | h+ ade6-M210 or ade6-M216 ura4-D18 leu1-32 rps25Δ::kanMX4 |
strain, strain background (S. pombe) | GST-rps2302-kanr | this study | | PEY1816: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 GST-rps2302- TADH1-PURA4-kanr-TTEF |
strain, strain background (S. pombe) | rps2302+-kanr | this study | | PEY1817: h+ his3-D1 leu1-32 ura4-D18 ade6-M210 rps2302+- TADH1-PURA4-kanr-TTEF |
strain, strain background (S. pombe) | ofd1Δ sre1N | PMID: 18418381 | | PEY873: h- his3-D1 leu1-32 ura4-D18 ade6-M210 ofd1Δ:: kanMX6 sre1(aa1-440) |
strain, strain background (S. pombe) | sre1N | PMID: 18418381 | | PEY875: h- his3-D1 leu1-32 ura4-D18 ade6-M210 sre1(aa1-440) |
strain, strain background (S. pombe) | 2XSRE-ura4+ sre1N | PMID: 15797383 | | PEY1290: h+ his-D1, leu1-32, ade6-M210, ura4-D18::2xSRE- ura4+-kanMX, sre1(aa1-440) |
strain, strain background (S. pombe) | 2XSRE-ura4+ sre1Δ | this study | | PEY1489: h+ his-D1, leu1-32, ade6-M210, ura4-D18::2xSRE- ura4+-kanMX, sre1Δ::kanMX6 |
strain, strain background (S. pombe) | nro1Δ sre1N | PMID: 15797383 | | PEY1410: h- his3-D1 leu1-32 ura4-D18 ade6-M210 nro1Δ:: kanMX4 sre1(aa1-440) |
strain, strain background (S. cerevisiae) | Yeast two-hybrid | PMID: 8978031 | | AH109: MATα trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MELTATA-lacZ |
recombinant DNA reagent | pSLF101 (vector) | PMID: 8332516 | | |
recombinant DNA reagent | pART1 (vector) | PMID: 3034608 | | |
recombinant DNA reagent | pGEX-4T3 (vector) | GE Healthcare Life Sciences | | |
recombinant DNA reagent | pSLF172 (vector) | PMID: 9218719 | | |
recombinant DNA reagent | pProEX-HTb (vector) | Invitrogen | | |
recombinant DNA reagent | ppMAL-c5X (vector) | NEB | | |
recombinant DNA reagent | EV bait (vector) | Clontech Matchmaker Gal4 Two-hybrid | | Gal4_BD |
recombinant DNA reagent | EV prey (vector) | Clontech Matchmaker Gal4 Two-hybrid | | Gal4_AD |
recombinant DNA reagent | human RPS23 (cDNA) | Duke Screening Center | | |
recombinant DNA reagent | human OGFOD1 (cDNA) | Invitrogen Ultimate ORF | Invitrogen: 1OH23210 | |
recombinant DNA reagent | nro1+ (plasmid) | PMID: 21481773 | | Progenitors: PCR; pSLF101 vector |
recombinant DNA reagent | nro1 P6D, G8D, A11D, L15D (plasmids) | this study | | Progenitors: nro1+ plasmid |
recombinant DNA reagent | rps2302+, P4D, G6D, A9D, L13D (plasmids) | this study | | Progenitors: PCR; pART1 vector |
recombinant DNA reagent | GST-Rps23/Nro1 bacterial expression plasmids | this study | | Progenitors: PCR; pGEX-4T3 vector |
recombinant DNA reagent | GST-rps2302 (plasmid) | this study | | Progenitors: PCR; pSLF172 vector |
recombinant DNA reagent | GST (plasmid) | this study | | Progenitors: PCR; pSLF172 vector |
recombinant DNA reagent | sre1N (plasmid) | PMID: 15797383 | | Progenitors: PCR; pSLF101 vector |
recombinant DNA reagent | ofd1 bait (plasmid) | this study | | Progenitors: PCR; Gal4_BD vector |
recombinant DNA reagent | OGFOD1 bait (plasmid) | this study | | Progenitors: OGFOD1 cDNA; Gal4_BD vector |
recombinant DNA reagent | rps23 pombe prey (plasmid) | this study | | Progenitors: PCR; Gal4_AD vector |
recombinant DNA reagent | RPS23 human prey (plasmid) | this study | | Progenitors:RPS23 cDNA; Gal4_AD vector |
recombinant DNA reagent | MBP-Rps23 (plasmid) | this study | | Progenitors: PCR; pMAL-c5X vector |
recombinant DNA reagent | ofd1 bacterial expression plasmid | PMID: 21481773 | | Progenitors: PCR; pProEX-HTb vector |
recombinant DNA reagent | nro1 bacterial expression plasmid | PMID: 21481773 | | Progenitors: PCR; pProEX-HTb vector |
recombinant DNA reagent | 6xHis-RPS23 (plasmid) | this study | | Progenitors: RPS23 cDNA; pProEX-HTb vector |
antibody | IRDye800CW/IRDy e680RD secondaries | LI-COR | | (1:20000) |
antibody | anti-Nro1 (rabbit polyclonal) | PMID: 15797383 | | (1:10000) |
antibody | anti-Dsc5 (rabbit polyclonal) | PMID: 22086920 | | (1:10000) |
antibody | anti-Sre1 (rabbit polyclonal) | PMID: 15797383 | | (1:2500) |
antibody | anti-Ofd1 (rabbit polyclonal) | PMID: 18418381 | | (1:10000) |
antibody | anti-GST (mouse monoclonal) | Covance | Covance:MMS-112R | (1:1000) |
antibody | anti-GFP (mouse monoclonal) | Roche Applied Science | Roche:1814460 | (1:1000) |
antibody | anti-Rps23 (mouse monoclonal) | Santa Cruz | Santa Cruz:sc-100837 | (1:200) |
antibody | anti-Rps5 (mouse monoclonal) | Santa Cruz | Santa Cruz:sc-390935 | (1:100) |
antibody | anti-actin (mouse monoclonal) | Santa Cruz | Santa Cruz:sc-47778 | (1:200) |
Chemical compound, drug | DMOG | Frontier Scientific | Frontier Scientific:D1070 | |
Chemical compound, drug | DSP | Thermo Fisher Pierce | Thermo Fisher Pierce:22586 | |
Chemical compound, drug | Heavy lysine | Cambridge Isotope Laboratories | Cambridge Isotope Laboratories: CNLM-291-H-1 | |
Chemical compound, drug | LMB | Cell Signaling | Cell Signaling:9676 | |