(A) Residues in the S1-M1 linker (Gln508, Ser509, and Lys510 represented as yellow atomic spheres) are in close proximity to the L2 of TARPs (L2 of γ2 is shown in red). (B) Residues in the S2-M4 linker (Lys781, Glu782 and Lys783) predicted to interact with L2 are labeled and shown as yellow atomic spheres. (C) Example responses from linker mutants coexpressed with γ2, γ8 and loop 1 chimeras to 500 ms 10 mM Glutamate. (D) Representative responses from linker mutants coexpressed with γ2, γ8 and loop 1 chimeras to a 7 s pulse of 10 mM Glutamate. The extent of superactivation is indicated. (E) Bar graphs summarizing the desensitization properties (top panel), steady state current (central) and superactivation (bottom). Colors are as in panel C. Filled symbols correspond to the traces shown in panels C and D; dashed lines represent GluA2 wild-type. ***p<0.001, **p<0.01, *p<0.05, against γ2; ∆∆∆ p<0.001, ∆ p<0.05, against γ8. Source data for panel E is found in Table 1–source data 1. Error bars represent s.e.m.