(A–E) The expression pattern of R30B01-AD ∩ R31H10-DBD in sensory neurons of the wings and halteres. Native GFP fluorescence is shown in green and autofluorescence from the cuticle is in magenta. Maximum intensity projections are shown. The proximal wing is to the right and the distal wing is to the left. (A) Sensory neurons on the wing. White boxes and letters indicate the regions shown in B–D. The different symbols indicate the sensory neuron types on the wing as proximal campaniform sensilla (white asterisks), distal campaniform sensilla (yellow asterisks), or bristle mechanosensory (white arrowheads). Scale bar, 250 μm. (B–D) Larger images of the regions shown in A. Scale bars, 50 μm. Shown are the proximal campaniform sensilla (B), distal campaniform sensilla (C), and bristle mechanosensory neurons (D). (E) Expression in the haltere campaniform sensilla (asterisks). Scale bar, 100 μm (F) CNS expression visualized by co-stain with anti-GFP (green) and anti-Bruchpilot (magenta). Arrows indicate the CNS entry points of afferents from the wings and halteres, and the location of ascending projections from some of these afferents. Scale bar, 100 μm. (G) Wing grooming bout rate with optogentic activation of neurons targeted by R30B01-AD ∩ R31H10-DBD. Data are displayed as described for Figure 3C. Asterisks: p<0.0001.