(A–E) Peripheral expression pattern of R52A06-GAL4 expressing green fluorescent protein (GFP). Confocal maximum projections are shown. Sensory neurons are in green and autofluorescence from the …
(A) Sensory neurons project their axons (afferents) to specific regions of the central nervous system (CNS) depending on which body part they are from. The neuropile of the CNS visualized with …
(A–B) A fiber optic probe that was connected to a blue light LED was used to direct light to specific body regions. Images show illumination of the posterior (A) or anterior (B) dorsal surfaces of …
(A–B) Head (magenta) or posterior body grooming movements (green) elicited with red light-illumination of R52A06-, R30B01-, and R81E10-GAL4 flies expressing CsChrimson. The movements are mutually …
(A) Ethograms showing head (magenta) or posterior body grooming (green) with red light-illumination of control flies. Ethograms of individual flies are stacked on top of each other. (B) Histogram …
(A–B) Box plots show the percent time flies spent performing head (A) or posterior grooming (B) with optogentic activation of neurons targeted by 52A06-, 30B01-, and 81E10-GAL4 (from experiment …
(A) Ethograms showing different grooming movements elicited with red light-illumination of R52A06-, R30B01-, or R81E10-GAL4 flies expressing CsChrimson. Ethograms of ten individual flies are stacked …
Head (magenta) or posterior body grooming (green) elicited with red light-illumination of R30B01-GAL4 flies expressing CsChrimson. Only flies that performed posterior body grooming at the moment of …
(A–B) The expression pattern of VT17251-LexA in eye bristle mechanosensory neurons. The neurons were stained with anti-GFP (green) and the brain neuropile is stained with anti-Bruchpilot (magenta). …
(A–E) The expression pattern of R30B01-AD ∩ R31H10-DBD in sensory neurons of the wings and halteres. Native GFP fluorescence is shown in green and autofluorescence from the cuticle is in magenta. …
CNS (A) and wing (B) expression patterns of R31H10-GAL4 that targets neurons whose activation elicits wing grooming. Maximum intensity projections are shown. (A) The CNS expression is visualized by …
(A) Ethograms (left) and histograms (right) showing eye grooming (magenta) or wing grooming (green) elicited with red light-activated CsChrimson expressed in different transgenic lines. The lines …
(A) The mean fraction of flies performing head or posterior grooming for each frame before, during, and after the three different stimuli shown in Figure 5A (bottom). The envelope shows the standard …
ChR2 was expressed in sensory neurons across the body using R52A06-GAL4.
ChR2 was expressed in sensory neurons across the body using R52A06-GAL4.
CsChrimson was expressed in sensory neurons using R52A06-GAL4. The infrared light in the bottom right corner indicates when the red light was on to activate the sensory neurons.
CsChrimson was expressed in sensory neurons using R30B01-GAL4. The infrared light in the bottom right corner indicates when the red light was on to activate the sensory neurons.
CsChrimson was expressed in sensory neurons using R81E10-GAL4. The infrared light in the bottom right corner indicates when the red light was on to activate the sensory neurons.
CsChrimson was expressed in eye bristle mechanosensory neurons using VT17251-LexA. The infrared light in the bottom right corner indicates when the red light was on to activate the sensory neurons.
CsChrimson was expressed in wing and haltere sensory neurons using R30B01-AD ∩ R31H10-DBD. The infrared light in the bottom right corner indicates when the red light was on to activate the sensory …
CsChrimson was expressed in eye, wing, and haltere sensory neurons using R31H10-AD ∩ R34E03-DBD. The infrared light in the bottom right corner shows when the red light was on to activate the sensory …