(A–D) Quantification of OA-induced follicle rupture (A, C) and the egg-laying number (B, D) from females with FC1 (A–B) and FC2 (C–D) Gal4 driving hntRNAi and/or hntEP55 overexpression. (E–L) Hnt expression (green, white in insets of outlined area) in stage-14C control (E, I), hntRNAi1 (F, J), hntRNAi1/hntEP55 (G, K), and hntEP55 (H, L) follicles with FC1 (FC1 > RFP in red, (E–H) or FC2 (FC2 > RFP in red, (I–L). (M–P’) Hnt expression (green in M-P and white in M’-P’) in stage-10B (M–N) and stage-14B (O–P) egg chambers. Hnt is highly overexpressed in a flip-out clone with actin-Gal4 and hntEP55 (M and O) but is barely detected in the clone with actin-Gal4 and hntEP55/hntRNAi1 (N and P). Clone cells are marked by act-Gal4 driving UAS-RFP expression (red) and DNA is labeled with DAPI in blue (M–P). *p<0.05, **p<0.01, ***p<0.001.