Msn2/4 regulate expression of glycolytic enzymes and control transition from quiescence to growth

  1. Zheng Kuang
  2. Sudarshan Pinglay
  3. Hongkai Ji  Is a corresponding author
  4. Jef D Boeke  Is a corresponding author
  1. NYU Langone Medical Center, United States
  2. Johns Hopkins University School of Public Health, United States

Abstract

Nutrient availability and stresses impact a cell's decision to enter a growth state or a quiescent state. Acetyl-CoA stimulates cell growth under nutrient-limiting conditions, but how cells generate acetyl-CoA under starvation stress is less understood. Here, we show that general stress response factors, Msn2 and Msn4, function as master transcriptional regulators of yeast glycolysis via directly binding and activating genes encoding glycolytic enzymes. Yeast cells lacking Msn2 and Msn4 exhibit prevalent repression of glycolysis genes and a significant delay of acetyl-CoA accumulation and reentry into growth from quiescence. Thus Msn2/4 exhibit a dual role in activating carbohydrate metabolism genes and stress response genes. These results suggest a possible mechanism by which starvation-induced stress response factors may prime quiescent cells to reenter growth through glycolysis when nutrients are limited.

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Article and author information

Author details

  1. Zheng Kuang

    Institute for Systems Genetics, NYU Langone Medical Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5855-8371
  2. Sudarshan Pinglay

    Institute for Systems Genetics, NYU Langone Medical Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8781-1476
  3. Hongkai Ji

    Department of Biostatistics, Johns Hopkins University School of Public Health, Baltimore, United States
    For correspondence
    hji@jhu.edu
    Competing interests
    The authors declare that no competing interests exist.
  4. Jef D Boeke

    Institute for Systems Genetics, NYU Langone Medical Center, New York, United States
    For correspondence
    jef.boeke@nyumc.org
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5322-4946

Funding

National Institutes of Health (U54GM103520)

  • Zheng Kuang
  • Jef D Boeke

National Institutes of Health (R01HG006841)

  • Zheng Kuang
  • Hongkai Ji

National Institutes of Health (R01HG006282)

  • Zheng Kuang
  • Hongkai Ji

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2017, Kuang et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Zheng Kuang
  2. Sudarshan Pinglay
  3. Hongkai Ji
  4. Jef D Boeke
(2017)
Msn2/4 regulate expression of glycolytic enzymes and control transition from quiescence to growth
eLife 6:e29938.
https://doi.org/10.7554/eLife.29938

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https://doi.org/10.7554/eLife.29938