Scc2/Nipbl hops between chromosomal cohesin rings after loading
- University of Oxford, United Kingdom
- Istituto Scientifico Ospedale San Raffaele, Italy
Abstract
The cohesin complex mediates DNA-DNA interactions both between (sister chromatid cohesion) and within chromosomes (DNA looping). It has been suggested that intra-chromosome loops are generated by extrusion of DNAs through the lumen of cohesin's ring. Scc2 (Nipbl) stimulates cohesin's ABC-like ATPase and is essential for loading cohesin onto chromosomes. However, it is possible that the stimulation of cohesin's ATPase by Scc2 also has a post-loading function, for example driving loop extrusion. Using fluorescence recovery after photobleaching (FRAP) and single- molecule tracking, we show that Scc2 binds dynamically to chromatin, principally through an association with cohesin. Scc2's movement within chromatin is consistent with a 'stop-and-go' or 'hopping' motion. We suggest that a low diffusion coefficient, a low stoichiometry relative to cohesin, and a high affinity for chromosomal cohesin enables Scc2 to move rapidly from one chromosomal cohesin complex to another, performing a function distinct from loading.
Article and author information
Author details
Funding
Wellcome (091859/Z/10/Z)
- Kim A Nasmyth
H2020 European Research Council (294401)
- Kim A Nasmyth
Cancer Research UK (C573/A12386)
- Kim A Nasmyth
Wellcome (101636/Z/13/Z)
- Stephan Uphoff
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Rhodes et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 5,261
- views
-
- 746
- downloads
-
- 97
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 97
- citations for umbrella DOI https://doi.org/10.7554/eLife.30000
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Scc2/Nipbl hops between chromosomal cohesin rings after loading
eLife 6:e30000.
https://doi.org/10.7554/eLife.30000
