(A) Diagram of the transgenesis vector for creating the riCCAB double-cut CopyCat (CC) element, the full nomenclature for which (kniri<CC|gRNA-A,gRNA-B|3xP3-EGFP>), is denoted according the following convention that we propose for representing active genetic elements in this and future studies. We have previously outlined features of a variety of active genetic elements including: MCRs, ERACR type reversal drives, CopyCat (CC) elements, and Trans-complementing drives (TC) (Gantz and Bier, 2016). Our proposed nomenclature convention for active genetic elements uses ‘<>” to denote that the element is ‘active’: gene locus<construct type (i.e. MCR, ERACR, CC, TC)| gRNAs| dominant marker| other 5' or 3' cargo>. (B) Homozygous riCC-AB adult wing (compare to Figure 1C). (C) Crossing scheme: Homozygous y1-MCR; +/+females were crossed to homozygous +/Y; riCC-AB/riCC-AB males and resulting F1 female progeny of the genotype y1-MCR/+; riCCAB/+were then crossed to w-/Y; +/+males. F2 female progeny were then scored for inheritance of the y1-MCR element (assessed by a full body yellow- phenotype) as well as for the EGFP-marked riCC-AB element. In addition, F2 females were scored for the presence (weak or strong) or absence of an ri phenotype. (D) Table of nine crosses showing the fraction of y- females which inherited the GFP marker (number and %GFP+) and among those that did, the numbers and percentages of those with wild-type, weak ri, strong ri, or total with ri wing phenotypes, and percent of total progeny with ri phenotypes. Results from the table shown in panel (D) can be summarized as follows: In a total of nine separate crosses, transmission of the y1-MCR was observed in 270/272 female progeny (=99.3%). Two individuals derived from cross 10–5* were y+. In four of the crosses the riCC-AB element was transmitted to 100% of the progeny, in three crosses to an average of 89% of progeny, and in two crosses only Mendelian inheritance was observed (note: nearly identical percentages were observed for riCC-AB transmission in males from the same set of 9 crosses). Note that there is no correlation between the % GFP +and the % ri (weak +strong). In particular, approximately 70% of progeny exhibited some degree of ri phenotype in all four crosses that transmitted the riCCAB to 100% of their progeny as well as in the two crosses that only transmitted the riCCAB to ~50% of their progeny. These results suggest that the germline and somatic activities of this active genetic element seem separable events.