(a) Inter-residue distance profiles for fourteen representative sequences, each 40-residues long. The legend shows the fraction of charged residues within each linker. The green dashed curve shows the inter-residue distance profile for the reference FRC limit. (b) Summary of the variation of ∆ as a function of the fraction of charged residues for the fourteen representative sequences. Here, ∆ , N is the number of linker residues,is the average spatial separation between residue pairs that are k apart in the linear sequence, is the corresponding spatial separation for a FRC chain, and the summation index k runs across all sequence-separations. Linkers for which ∆ < –0.1 will have negative effective solvation volumes (ves < 0); linkers for which –0.1 ≤ ∆≤0.1 will have near zero effective solvation volumes (ves ≈ 0); and linkers for which ∆>0.1, will have positive effective solvation volumes (ves > 0). For the self-avoiding random coil (SARC) linkers, ∆ ≈ 0.5 and this is shown as a horizontal red line. (c) Length distribution of all 226 unique disordered linkers. (d) Distribution of ∆ values extracted from all-atom simulations of all 226 linkers. Based on the results shown in panel (B), we delineate the ∆-distribution into three regimes: ∆ < –0.1 (blue bars), –0.1 ≤ ∆≤0.1 (green bars), and ∆>0.1 (red bars). These regimes correspond, respectively to linkers for which ves is less than zero, near zero, or greater than zero.