1. Cell Biology
  2. Developmental Biology
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Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii

  1. B Duygu Özpolat  Is a corresponding author
  2. Mette Handberg-Thorsager
  3. Michel Vervoort
  4. Guillaume Balavoine  Is a corresponding author
  1. Institut Jacques Monod, France
  2. Max Planck Institute of Molecular Cell Biology and Genetics, Germany
Research Article
  • Cited 7
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Cite this article as: eLife 2017;6:e30463 doi: 10.7554/eLife.30463

Abstract

Cell lineage, cell cycle, and cell fate are tightly associated in developmental processes, but in vivo studies at single-cell resolution showing the intricacies of these associations are rare due to technical limitations. In this study on the marine annelid Platynereis dumerilii, we investigated the lineage of the 4d micromere, using high-resolution long-term live imaging complemented with a live-cell cycle reporter. 4d is the origin of mesodermal lineages and the germline in many spiralians. We traced lineages at single-cell resolution within 4d and demonstrate that embryonic segmental mesoderm forms via teloblastic divisions, as in clitellate annelids. We also identified the precise cellular origins of the larval mesodermal posterior growth zone. We found that differentially-fated progeny of 4d (germline, segmental mesoderm, growth zone) display significantly different cell cycling. This work has evolutionary implications, sets up the foundation for functional studies in annelid stem cells, and presents newly-established techniques for live-imaging marine embryos.

Article and author information

Author details

  1. B Duygu Özpolat

    Institut Jacques Monod, Paris, France
    For correspondence
    dozpolat@gmail.com
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1900-965X
  2. Mette Handberg-Thorsager

    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Michel Vervoort

    Institut Jacques Monod, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Guillaume Balavoine

    Institut Jacques Monod, Paris, France
    For correspondence
    Guillaume.BALAVOINE@ijm.fr
    Competing interests
    The authors declare that no competing interests exist.

Funding

Labex Who am I (No.ANR-11-LABX-0071)

  • Michel Vervoort
  • Guillaume Balavoine

Agence Nationale de la Recherche (METAMERE no. ANR-12-BSV2-0021)

  • Michel Vervoort
  • Guillaume Balavoine

Agence Nationale de la Recherche (TELOBLAST no. ANR-16-CE91-0007)

  • B Duygu Özpolat
  • Michel Vervoort
  • Guillaume Balavoine

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Lilianna Solnica-Krezel, Washington University School of Medicine, United States

Publication history

  1. Received: July 22, 2017
  2. Accepted: December 11, 2017
  3. Accepted Manuscript published: December 12, 2017 (version 1)
  4. Version of Record published: January 11, 2018 (version 2)

Copyright

© 2017, Özpolat et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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