Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii
- Institut Jacques Monod, France
- Max Planck Institute of Molecular Cell Biology and Genetics, Germany
Abstract
Cell lineage, cell cycle, and cell fate are tightly associated in developmental processes, but in vivo studies at single-cell resolution showing the intricacies of these associations are rare due to technical limitations. In this study on the marine annelid Platynereis dumerilii, we investigated the lineage of the 4d micromere, using high-resolution long-term live imaging complemented with a live-cell cycle reporter. 4d is the origin of mesodermal lineages and the germline in many spiralians. We traced lineages at single-cell resolution within 4d and demonstrate that embryonic segmental mesoderm forms via teloblastic divisions, as in clitellate annelids. We also identified the precise cellular origins of the larval mesodermal posterior growth zone. We found that differentially-fated progeny of 4d (germline, segmental mesoderm, growth zone) display significantly different cell cycling. This work has evolutionary implications, sets up the foundation for functional studies in annelid stem cells, and presents newly-established techniques for live-imaging marine embryos.
Data availability
-
Sample A - Z-stacksAvailable at Zenodo under a Creative Commons Attribution-Non Commercial-No Derivatives license.
Article and author information
Author details
Funding
Labex Who am I (No.ANR-11-LABX-0071)
- Michel Vervoort
- Guillaume Balavoine
Agence Nationale de la Recherche (METAMERE no. ANR-12-BSV2-0021)
- Michel Vervoort
- Guillaume Balavoine
Agence Nationale de la Recherche (TELOBLAST no. ANR-16-CE91-0007)
- B Duygu Özpolat
- Michel Vervoort
- Guillaume Balavoine
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Özpolat et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 3,837
- views
-
- 465
- downloads
-
- 42
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 42
- citations for umbrella DOI https://doi.org/10.7554/eLife.30463
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii
eLife 6:e30463.
https://doi.org/10.7554/eLife.30463
