Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
- University Medical Center Groningen, Netherlands
- Terry Fox Laboratory, BC Cancer Agency, Canada
Abstract
Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.
Article and author information
Author details
Funding
European Research Council (Advanced Grant)
- Peter M Lansdorp
Netherlands Organisation for Scientific Research (Vidi Grant 016.131.314)
- Michael Chang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Lorraine Symington, Columbia University
Version history
- Received: July 19, 2017
- Accepted: December 8, 2017
- Accepted Manuscript published: December 12, 2017 (version 1)
- Version of Record published: December 18, 2017 (version 2)
Copyright
© 2017, Claussin et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
eLife 6:e30560.
https://doi.org/10.7554/eLife.30560
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