Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq

  1. Clémence Claussin
  2. David Porubský
  3. Diana CJ Spierings
  4. Nancy Halsema
  5. Stefan Rentas
  6. Victor Guryev
  7. Peter M Lansdorp
  8. Michael Chang  Is a corresponding author
  1. University Medical Center Groningen, Netherlands
  2. Terry Fox Laboratory, BC Cancer Agency, Canada

Abstract

Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.

Article and author information

Author details

  1. Clémence Claussin

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0994-3596
  2. David Porubský

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  3. Diana CJ Spierings

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  4. Nancy Halsema

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  5. Stefan Rentas

    Terry Fox Laboratory, BC Cancer Agency, Vancouver, Canada
    Competing interests
    The authors declare that no competing interests exist.
  6. Victor Guryev

    European Research Institute for the Biology of Ageing, University Medical Centre Groningen, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  7. Peter M Lansdorp

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7435-1071
  8. Michael Chang

    European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands
    For correspondence
    m.chang@umcg.nl
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1706-3337

Funding

European Research Council (Advanced Grant)

  • Peter M Lansdorp

Netherlands Organisation for Scientific Research (Vidi Grant 016.131.314)

  • Michael Chang

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Lorraine Symington, Columbia University

Publication history

  1. Received: July 19, 2017
  2. Accepted: December 8, 2017
  3. Accepted Manuscript published: December 12, 2017 (version 1)
  4. Version of Record published: December 18, 2017 (version 2)

Copyright

© 2017, Claussin et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Clémence Claussin
  2. David Porubský
  3. Diana CJ Spierings
  4. Nancy Halsema
  5. Stefan Rentas
  6. Victor Guryev
  7. Peter M Lansdorp
  8. Michael Chang
(2017)
Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
eLife 6:e30560.
https://doi.org/10.7554/eLife.30560
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