1. Structural Biology and Molecular Biophysics
  2. Microbiology and Infectious Disease
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Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands

  1. Altaira D Dearborn
  2. Erin A Wall
  3. James L Kizziah
  4. Laura Klenow
  5. Laura K Parker
  6. Keith A Manning
  7. Michael S Spilman
  8. John M Spear
  9. Gail E Christie
  10. Terje Dokland  Is a corresponding author
  1. National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, United States
  2. Virginia Commonwealth University, United States
  3. University of Alabama, United States
  4. Direct Electron, United States
  5. Florida State University, United States
Research Article
Cite this article as: eLife 2017;6:e30822 doi: 10.7554/eLife.30822
10 figures, 3 tables, 4 data sets and 2 additional files

Figures

Schematic overview of SaPI1’s molecular piracy of 80α.

Only part of the 80α genome is shown for clarity, and relevant genes are labeled: sri, SaPI1 derepressor; terS, terminase small subunit; terL, terminase large subunit; PP, portal protein; 44, minor capsid protein gp44; SP, scaffolding protein; CP, major capsid protein. First, the 80α-encoded protein Sri interacts with the SaPI1 master repressor Stl (1), causing derepression of the rightwards operon that includes the transcriptional activator str, the excisionase xis and the replicase (pri and rep). Second, the SaPI1-encoded proteins CpmA and CpmB re-direct the capsid assembly pathway to form small capsids (2). Finally, the SaPI1-encoded TerS interacts with 80α-encoded TerL to cause specific packaging of SaPI1 DNA (3). Also labeled are the SaPI1 integrase (int), genes encoding staphylococcal enterotoxins K (sek) and Q (seq), and the toxic shock syndrome toxin (tst) gene.

https://doi.org/10.7554/eLife.30822.002
Cryo-EM data.

(A, B) Electron micrographs of 80α (A) and SaPI1 (B) procapsids. Scale bar, 100 nm. (C, D) Fourier Shell Correlation (FSC) curves between maps calculated from the two half datasets (blue) and between the model and the map (orange) for 80α (A) and SaPI1 (B). The resolution limits at 0.143 cutoff (dashed line) are indicated. The discrepancy between the model and the map for SaPI1 is likely due to the disordered and unmodeled portions of the CpmB protrusions.

https://doi.org/10.7554/eLife.30822.003
Figure 3 with 2 supplements
Reconstruction of 80α and SaPI1 procapsids.

(A, B) Isosurface representation of the 80α (A) and SaPI1 (B) reconstructions, colored radially on an absolute scale (color bar). The triangles represent one icosahedral face, delimited by three fivefold axes. Icosahedral twofold and threefold symmetry axes are indicated by ovals and triangles, respectively. (C, D) Ribbon representations of the C subunits of the 80α (C) and SaPI1 (D) atomic models. N-arm, red; P domain, green; E-loop, yellow; A-domain, blue. SP (in 80α), purple; CpmB (in SaPI1), orange. Locations of α-helices α3 (the spine helix), α5 and α6, and the A-loop are also indicated in (C). (D) Isosurface representation of a 30 Å thick slice through the SaPI1 procapsid map, showing the protruding domains on the inside of the shell (arrows), colored radially on the same scale as in (B). (G) Electrostatic surface of the 80α CP subunit C, colored according to charge (blue, most positive; red, most negative).

https://doi.org/10.7554/eLife.30822.004
Figure 3—figure supplement 1
Electron density and model.

(A, B) Isosurface representation (mesh) of one whole subunit from 80α (A) and SaPI1 (B), with the corresponding CP model in backbone representation. The surrounding density is shown as a semi-transparent yellow isosurface. (C–F) Details of density and atomic model from 80α (C, E) and SaPI1 (D, F), corresponding to residues 68–84 in the E-loop (C, D) and 125–144 in the spine helix (α3) (E, F). Pertinent residues are labeled.

https://doi.org/10.7554/eLife.30822.005
Figure 3—figure supplement 2
Data analysis.

(A, B) FSC curves showing the correspondence of the model against the test dataset (FSCtest, blue) and the working dataset (FSCwork, orange), after refinement against only the working data set for the 80α (A) and SaPI1 (B) procapsid reconstructions. (C, D) Isosurface representation of one asymmetric unit from the 80α (C) and SaPI1 (D) procapsid maps, colored by local resolution from 3 Å to >6 Å according to the color bar, calculated using ResMap (Kucukelbir et al., 2014).

https://doi.org/10.7554/eLife.30822.006
Comparison of CP subunits.

(A, B) Superposition of the backbones of the seven subunits of 80α (A) and the four subunits of SaPI1 (B). Panels on the right are rotated by 90°. The two distinct conformations of the E-loop (‘UP’ and ‘DOWN’) are indicated on the 80α superposition. The subunits are colored according to the schematic diagrams on the left (A, red; B, blue; C, green; D, yellow; E, tan; F, orange; G, purple). The orange ovals in (B) represent the CpmB dimers. (C, D) Superpositions of the pentamers (C) and hexamers (D) of 80α (gray) and SaPI1 (blue), in ribbon representation.

https://doi.org/10.7554/eLife.30822.007
Interactions between capsid proteins.

(A, B) Ribbon representation of the 80α pentamer (A) and hexamer (B), showing the detailed interactions between the A-domains. Helices α5 and α6 and the A-loop are colored according to the color scheme in Figure 4A. The skew plane between B-C and E-F subunits is indicated by the dashed line. Pertinent residues are shown in stick representation and labeled in the expanded view on the right, showing the two distinct types of A-domain interactions. (C) Detail of the 80α model, showing the interactions between the E-loop of subunit D (yellow) and the P-domain and P-loop of subunit C (green). (D) Detail of the trimer interaction at the icosahedral threefold axis showing three E subunits (tan) and the corresponding adjacent F subunits (orange). Key residues involved in the threefold interaction, as well as W98, are indicated in stick model in the expanded view.

https://doi.org/10.7554/eLife.30822.009
Comparison of capsomer angles in 80α and SaPI1.

(A, B) Schematic diagrams showing the organization of hexamers and pentamers in the T = 7 (A) and T = 4 (B) lattices. The dihedral angles between hexamers are defined by the Greek letters α–δ. Type 1, 2 and 3 threefold axes are indicated. (C) Table of the calculated interior inter-capsomeric dihedral angles in 80α and SaPI1. The Δ column indicates the difference between corresponding angles. (D) Ribbon representation of a slab through one half of the 80α (gray) and SaPI1 (blue) shells, showing how the difference in inter-capsomer angles are propagated through the lattice. The models were aligned at one fivefold vertex (shown at bottom of diagram) and the planes representing the capsomers (gray and blue circles) and the resulting α and β angles are shown.

https://doi.org/10.7554/eLife.30822.010
Interaction of CP with SP and CpmB.

(A) Ribbon diagram of CP and SP from subunit C of the 80α procapsid with the density corresponding to SP superimposed, colored as in Figure 3E. (B) Detail of the SP-CP interaction, with relevant residues highlighted (stick representation) and labeled. Key interactions (<4 Å) are indicated by stippled lines. (C) Ribbon diagram of CP and CpmB from subunit C in the SaPI1 procapsid showing the density corresponding to CpmB, colored as in Figure 3F. The NMR structure of the N-terminal dimerization domain of CpmB (salmon) has been placed in the density, but could not be modeled accurately. (D) Detail of CpmB-CP interaction with relevant residues and distances (<4 Å) indicated. (E) Superposition of CpmB (blue) and SP (gray). Side chains in CpmB for residues that differ from their equivalents in SP are shown in red. (F) Ribbon diagram showing the interaction of one CpmB dimer (orange and salmon) with two adjacent hexameric capsomers. The C and D subunits are colored (green and yellow, respectively); other subunits are in gray. The positions of two twofold and one threefold symmetry axis are indicated.

https://doi.org/10.7554/eLife.30822.011
Sequence alignment of the C-terminal domains of SP and CpmB.

Size redirection negative mutations in CpmB and lethal mutations in SP are indicated (red). The mutations in CP that suppressed the I203T mutant are listed (green). Alignment generated in ClustalW and rendered with ESPript 2.0.

https://doi.org/10.7554/eLife.30822.013
Electron micrographs of negatively stained particles from mutant lysates.

(A) Strain ST368 (80α SP I203T + wildtype SaPI1); (B) strain ST454 (80α SP A198I + wildtype SaPI1; (C) strain ST467 (80α SP-CpmBCTD + wildtype SaPI1); (D) strain ST458 (80α wildtype + SaPI1 CpmB-SPCTD). Examples of small procapsids (sp), small virions (sv), and large procapsids (lp) are indicated on the images. Scale bar denotes 100 nm.

https://doi.org/10.7554/eLife.30822.015
Schematic model for scaffolding competition-mediated capsid size redirection.

In this scheme, SP is needed for the correct folding of CP (1), which is only stable as an oligomer. The E-loop conformation is ‘up’ or ‘down’ depending on its binding to a neighboring CP subunit (2). SP might be involved in this conformational switching. Subunits are assembled into hexamers (and possibly other oligomers), which are independent of the presence or absence of CpmB (3). The curvature imposed on the nascent shell by the trivalent interaction of hexamers (4) forces the incorporation of pentamers into the lattice at specific points (5), leading to a T = 7 shell. The presence of CpmB dimers imposes a sharper angle between hexamers (6), leading to the more frequent incorporation of pentamers and the formation of a the smaller, T = 4 shell. CpmA is thought to facilitate the removal of SP to provide access for CpmB (6).

https://doi.org/10.7554/eLife.30822.016

Tables

Table 1
Root-mean square deviation (RMSD) values (in Å) between subunit pairs within 80α, within SaPI1 and between 80α and SaPI1.

Values for ‘unpruned’ structures include all atoms when determining the best fit. Values for ‘pruned’ structures only include the best matching residue pairs. Pruning was done in UCSF Chimera using standard parameters. For 80α, pairs of subunits related across the quasi-twofold axis are highlighted in green. Comparison of subunits for which the E-loops are in the ‘UP’ conformation are highlighted in blue.

https://doi.org/10.7554/eLife.30822.008
Root mean square deviation between CP subunits
PrunedUnpruned
# res.RMSD (Å)# res.RMSD (Å)
80α procapsid
 subAsubB2150.9862842.927
 subAsubC2500.8972841.521
 subAsubD2390.9432842.902
 subAsubE2180.9892842.900
 subAsubF2520.8992841.533
 subAsubG2391.0242842.878
 subBsubC2240.8812843.072
 subBsubD2290.9552841.901
 subBsubE2510.7272841.506
 subBsubF2190.9072843.244
 subBsubG2320.9092841.686
 subCsubD2410.8532843.002
 subCsubE2250.9232843.108
 subCsubF2710.7642840.987
 subCsubG2370.8602842.974
 subDsubE2311.0012841.715
 subDsubF2460.7812843.195
 subDsubG2620.7042841.302
 subEsubF2200.9792843.257
 subEsubG2360.9812841.654
 subFsubG2420.8472843.104
SaPI1 procapsid
 subAsubB2181.0472842.656
 subAsubC2471.0922841.572
 subAsubD2231.1082842.862
 subBsubC2201.0402842.865
 subBsubD2311.0402841.633
 subCsubD2420.9212842.669
SaPI1 vs. 80α
SaPI180α
 subAsubA2590.9202841.495
 subBsubB2500.9262841.443
 subBsubE2570.8232841.380
 subCsubC2680.9352841.165
 subCsubF2680.9972841.190
 subDsubD2470.9482841.536
 subDsubG2620.9392841.170
Table 2
List of contacts between SP and CP in the 80α procapsid (panel A, left) and between CpmB and CP in the SaPI1 procapsid (panel B, right).

Most contacts are within the same subunit (chain C), but some contacts are made with the adjacent subunit (chain B). Corresponding residues in SP and CpmB are on the same row. The assigned SP sequence starts on P191, while CpmB starts on Q48. A contact is defined as an interatomic distance of ≤4 Å. The table lists the specific residues that are involved in these contacts and enumerates the residues (# Res) and atoms (# Atoms) that contribute to these contacts. Residues that make the most contacts (≥5 interatomic pairs) are highlighted in green, while those that display ≤1 contacts are highlighted in red. ‘Intermediate’ contacts (2–4) are shown in yellow.

https://doi.org/10.7554/eLife.30822.012
A. 80α procapsid. contacts (≤4 Å) between SP (subunit C) and CPB. SaPI1 procapsid. contacts (≤4 Å) between CpmB (subunit C) and CP
 SPCP chain CCP chain BResAtomsCpmBCP chain CCP chain BResAtoms
Q48N12015
E4900
E5000
Q51E117, N120213
S5200
K5300
Q54N4112
K55E67E117, Y12137
Q56Q125111
Y5700
 P19100G5800
 Q192E129, Y12125T5900
 N19300T6000
 L194T45, L48, I25336L61T4511
 A195Y6311Q62Y63, E64, P65317
 E19600N6300
 I197T45, Q49, M5234L64Q4917
 A198M52, Y6324A65M52, Y6325
 R199E50, P3225K66P13212
 Q20000Q6700
 K201Q49, E5326N68M52, E5325
 R202Q59, L60, G61, K62, G24754R69M58, Q59, L60, G6148
 I203Q59, V232, P23037I70Q59, P23026
 I204L60, N194, A195, P230411I71N194, P230K23533
 K20500K72K23511
 N206Y139, K23526
Table 3
Genetic analysis of 80α and SaPI1, listing the S. aureus strains and the genotypes of the 80α prophages and SaPI1 elements that they harbor.

SaPI1 also includes the tst::tetM insertion that confers tetracycline resistance. The corresponding phage titers (PFU/ml) and tetM transducing titers (TU/ml) are from filtered lysates resulting from induction with mitomycin C. Titers of mutations considered lethal or greatly impaired are shown in red; titers that are essentially wildtype are shown in green. WT = wildtype; N/D = not determined.

https://doi.org/10.7554/eLife.30822.014
StrainPhageSaPI1Phage titerSaPI1 titer
RN10616WT8.70E + 10
RN10628WTWT5.10E + 081.33E + 08
ST196SP R202K<10
ST197SP R202KWTN/D<10
ST278SP R202S<10
ST366SP R202SWT<101.67E + 01
ST279SP R202E<10
ST367SP R202EWTN/D<10
ST358SP I203T1.00E + 02
ST368SP I203TWT1.71E + 026.67E + 06
ST469SP I204L5.33E + 10
ST417SP A198I1.00E + 01
ST454SP A198IWT<104.18E + 06
ST384CP M52L1.30E + 10
ST415CP M52Q1.00E + 01
ST481CP M52QWT<103.00E + 01
ST385CP Y123C1.47E + 10
ST466SP::CpmBCTD2.70E + 09
ST467SP::CpmBCTDWT9.00E + 069.25E + 05
ST468SP::CpmBCTDCpmB::SPCTD6.80E + 069.00E + 05
ST458WTCpmB::SPCTD1.80E + 089.60E + 08
ST465SP R202ECpmB::SPCTD<101.40E + 02

Data availability

The following data sets were generated
  1. 1
  2. 2
  3. 3
  4. 4

Additional files

Supplementary file 1

List of primers used to construct the plasmids in this study.

Restriction sites are underlined.

https://doi.org/10.7554/eLife.30822.018
Transparent reporting form
https://doi.org/10.7554/eLife.30822.019

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