(A, B) Survival curves of mice infected ip with 2 × 103 blood trypomastigotes of the Y strain. (A) Myd88−/− (CD45.2+)→WT (B6 x B6.SJL F1, CD45.1+CD45.2+) and WT (B6.SJL, CD45.1+)→WT (B6 x B6.SJL F1, …
(A) Experimental design for mixed BM chimera construction. Irradiated WT (B6 x B6.SJL F1) mice were reconstituted with a 1:1 mix of WT (B6.SJL, CD45.1+) and Myd88−/− (CD45.2+) BM cells. (B) …
(A) Gate strategy and dot plot analysis of IFN-γ+CD4+ T cells among CD4+CD45.2+ (Myd88−/−) or among CD4+CD45.1+ (B6) plus CD4+CD45.2+CD45.1+(B6xB6.SJL F1) (WT) T cells in Rag2−/− mice, reconstituted …
(A) Gate strategy and dot plot analysis of IFN-γ+CD4+ T cells among CD8+CD45.2+ (Myd88−/−) or among CD8+CD45.1+ (B6) plus CD8+CD45.2+CD45.1+(B6xB6.SJL F1) (WT) T cells. (B–C) Mean frequencies and …
(A) Experimental design: CD4+CD45.1+ cells (WT) and CD4+CD45.2+ cells (Myd88−/−) were sorted (99% of purity) from the pool of splenocytes obtained from infected mixed BM chimeric mice at day 14 pi …
Table 1. Differential expressed genes between WT and Myd88−/− CD4+ T cells sorted from infected BM-mixed chimeras.
Interactions among the genes were assessed and visualized by Ingenuity Pathway Analysis (IPA) database. Darker colors represent higher expression in (A) Myd88−/− (blue) or in (B) WT (red) CD4+ T …
(A) Gate strategy and mean percentage of CD44hiCD4+ and T-bet+CD44hiCD4+ cells gated on CD4+ T splenocytes from non-infected controls and infected B6 mice at day 14 pi. (B) Representative dot plot …
(A, B) Serum levels of (A) IL-1β and (B) IL-18. (C) Mean percentages of IFN-γ+CD4+ T cells gated on splenic CD4+ T lymphocytes. Symbols represent the mean value of 4 individually analyzed B6 mice, …
(A) Representative dot plots showing gating strategy for CD4+ T cells and for CD45.1+ (B6.SJL WT) or CD45.2+ (B6 WT or Myd88−/− or Il1r1−/− or Il18r1−/−) splenocytes from mixed BM chimeras. (B) …
(A, C, G, E and I) Mean frequencies and (B, D, F, H and J) absolute cell numbers of: (A and B) BrdU+CD4+ and (C and D) CCR5+CD4+ cells gated on WT CD45.1+ or KO CD45.2+ CD4+ T cells, as in Figure 5A.…
Representative dot plots showing anti-BrdU staining in CD4+CD45.1+(B6.SJL, WT) or CD4+CD45.2+ (B6 WT or Myd88−/− or Il1r1−/−or Il18r1−/−) splenocytes from control or infected mixed BM chimeras, …
Representative dot plots showing CCR5 expression on CD4+CD45.1+ (B6.SJL, WT) or CD4+CD45.2+ (B6 WT or Myd88−/− or Il1r1−/−or Il18r1−/−) splenocytes, from control or infected mixed BM chimeras, gated …
(A) Gate strategy for CD45.1+ (B6.SJL WT) and CD45.2+ (B6 WT or Myd88−/− or Il1r1−/−or Il18r1−/−) expression on CD8+ T cells. (B) Representative dot plots of IFN-γ expression gated on CD8+CD45.1+ …
Representative dot plots showing anti-GzB staining in CD8+CD45.1+(B6.SJL, WT) or CD8+CD45.2+(B6 WT or Myd88−/− or Il1r1−/− or Il18r1−/−) splenocytes from control or infected mixed BM chimeras, gated …
Representative dot plots showing anti-CD44 staining on CD4+CD45.1+(B6.SJL, WT) or CD4+CD45.2+(B6 WT or Myd88−/− or Il1r1−/− or Il18r1−/−) splenocytes from control or infected mixed BM chimeras, …
(A) Parasitemia curve, (B) survival and (C) parasite load in the myocardium at day 14 pi of Il18r1−/−, Myd88−/− and WT B6 mice infected with 2 × 103 blood trypomastigotes of the Y strain. Survival …
(A and C) Survival; (B and D) mean parasitemia levels at day 9 pi of Il18r1−/− (A and B) or Myd88−/− mice (C and D), which received or not 1.3 × 106 CD4+ T cells purified from infected WT B6 mice. …
(A) Sorting of CD4+ T cells: Representative frequency of CD4+CD3+ T cells after enrichment by negative selection with magnetic beads (pre-sorting, left) followed by FACS sorting (post-sorting, …