(A, C, G, E and I) Mean frequencies and (B, D, F, H and J) absolute cell numbers of: (A and B) BrdU+CD4+ and (C and D) CCR5+CD4+ cells gated on WT CD45.1+ or KO CD45.2+ CD4+ T cells, as in Figure 5A. (E and F) IFN-γ+CD8+ and (G and H) GzB+CD8+ T cells gated on WT CD45.1+ or KO CD45.2+ CD8+ T cells, as shown in Figure 6—figure supplement 3. (I and J) CD44hiCD4+ T cells, gated on WT or KO CD4+ T cells as in Figure 5A. Bars are the mean of individually analyzed mice from one (C, D, I and J) or two combined independent experiments (A, B, E, F, G and H). (n = 3 to 8); error bars = SEM; ND = not done; ns = non significant; *p≤0.05; **p≤0.01; ***p≤0.001, ****p≤0.0001 (two-tailed Student t-test). Gate strategies and representative dot plots are shown in Figure 6—figure supplements 1–5.