1. Structural Biology and Molecular Biophysics
  2. Cell Biology
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Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles

  1. Franziska Decker
  2. David Oriola
  3. Benjamin Dalton
  4. Jan Brugués  Is a corresponding author
  1. Max Planck Institute of Molecular Cell Biology and Genetics, Germany
  2. Center for Systems Biology Dresden, Germany
  3. Max Planck Institute for the Physics of Complex Systems, Germany
Research Article
Cite this article as: eLife 2018;7:e31149 doi: 10.7554/eLife.31149
4 figures, 11 videos, 2 tables and 1 additional file

Figures

Figure 1 with 3 supplements
Microtubule nucleation in monopolar spindles is spatially regulated.

(A) Fluorescence image of a monopolar spindle (left), and single-molecule fluorescent tubulin and EB1-GFP (right). (B) Circular laser cut and corresponding differential intensity depolymerization front at different times. (C) Radial sum of differential intensities at different time points (from dark to light blue) of one cut at a radius of 19 μm from the center. The area under each curve equals the mass of microtubules depolymerized per time interval of 2 s. (D) Nucleation profile of monopolar spindles (N = 117 cuts, mean ± SD).

https://doi.org/10.7554/eLife.31149.003
Figure 1—figure supplement 1
Monopolar spindles have a similar size as regular spindles.

(A) Left, monopolar spindle assembled with 200 µM STLC and labeled with 150 nM atto-565 tubulin. Right, regular spindle. (B) Velocity distributions of speckles in a monopole obtained by analyzing three different regions from Video 2 (n = 717 speckles total). The velocity distributions correspond to the x and y-components given a cartesian reference frame. The x and y velocity components of a speckle trajectory were computed as the difference between the final and initial position of the speckle along a given component, divided by the total speckle lifetime. The mean speckle velocities along each component are vx = 0.09 ± 0.03 µm/min and vy = 0.08 ± 0.04 µm/min (mean ± SDM). The average modulus of the speckle velocity is v = 0.12 ± 0.07 µm/min.

https://doi.org/10.7554/eLife.31149.004
Figure 1—figure supplement 2
The depolymerization velocity of microtubules in monopolar spindles is indistinguishable from the one in spindles.

We plotted the position of the depolymerization wave after a cut, which was determined by the maximum of the fitted Gaussians, over time. Each line corresponds to one wave (i.e. one cut). Data for monopoles and spindles are shown in blue and orange, respectively. The slope of each dataset corresponds to the depolymerization velocity of the cut microtubules (mean depolymerization velocity ± SD, Nmonopole = 58, Nspindle = 22).

https://doi.org/10.7554/eLife.31149.005
Figure 1—figure supplement 3
Depolymerization wave of microtubules cut by laser ablation.

(A) Coordinate system for plotting differential intensities. Differential intensities I(x,ϕ), where x is the distance from the center of the monopole and ϕ is the angular coordinate, were integrated over ϕ(0<ϕ2π) for each value of x. The origin of the polar coordinate system was always set to be in the center of the monopolar spindle. Next, we plotted the integrated differential intensities as a function of x for each time point of the differential intensity movie (see Figure 1C). (B) Definitions of the variables used in the cutting method: xmxp are the locations of minus/plus-ends with respect to the center of the monopole, r is the radius of the cut, vd is the depolymerization velocity, and y is the position of the depolymerization front with respect to the center of the monopole. (C) The log-linear plot shows the normalized integrated areas of the Gaussian fits (Figure 1C) as a function of the distance that the wave travels away from the cut, given cuts performed at different locations (the lighter the color the further was the cut from the monopole center). Dashed lines represent linear fits to the log-linear data normalized such that the interception with the y-axis equals unity.

https://doi.org/10.7554/eLife.31149.006
Figure 2 with 1 supplement
Microtubule nucleation depends on the stability of microtubules.

(A) Inhibition of MCAK leads to larger steady-state monopoles. Scale bar = 20 µm (B) Microtubule length distributions measured from laser ablation and fitted to an exponential (mean ± SD). (C) Normalized histograms of microtubule lifetimes of control (N = 5331 speckles, five structures) and MCAK-inhibited monopoles (N = 7289 speckles, three structures), and corresponding first-passage time fits (see Methods and materials). (D) Nucleation profile of control (N = 117 cuts) and MCAK-inhibited monopoles (N = 74 cuts) in arbitrary units that are consistent in both structures (mean ± SD). The inset shows both nucleation profiles multiplied by the circumference length at each radius, which corresponds to the total microtubule nucleation at that distance from the center of the monopole.

https://doi.org/10.7554/eLife.31149.013
Figure 2—figure supplement 1
Microtubule length distributions are independent of the position in monopoles.

Microtubule length distributions measured from minus ends at locations 4.78 ± 0.01 µm, 8.2 ± 1.3 µm, 12.8 ± 2.0 µm, and 18.8 ± 1.4 µm from the center of the monopole (mean ± SD). The solid line corresponds to the exponential fit to the monopole data from Figure 2B.

https://doi.org/10.7554/eLife.31149.014
Figure 3 with 2 supplements
Microtubule nucleation requires physical proximity to pre-existing microtubules.

(A) Inert obstacles (fluorocarbon oil microdroplets or polystyrene beads) immobilized to coverslips selectively block microtubule nucleation. Left: Schematic outcomes depending on whether new microtubules are nucleated in physical proximity from pre-existing microtubules (top) or not (bottom). Middle: Two time points of a microtubule structure with labeled tubulin (red) and EB1-GFP (green) growing around immobilized frog sperm chromosomes. The oil microdroplet is highlighted with a dashed ellipse. Right: Normalized line profiles of shadow regions behind six different obstacles (colored dots). The intensity profiles were normalized to the average intensity outside the obstacles and rescaled to the radius of the obstacle. Gray lines show mean ± SD. (B) Left: Addition of RanQ69L to extract homogeneously activates nucleation and creates mini asters after 20 min (22). Middle: Addition of RanQ69L to monopoles leads to immediate growth of new microtubules from the pre-existing monopoles. Right: Quantification of radial fluorescence intensity profiles at different time points of the growing monopole shown in the middle.

https://doi.org/10.7554/eLife.31149.015
Figure 3—figure supplement 1
Growth of a monopolar spindle.

Integrated fluorescence intensity of z-stacks of a microtubule structure labeled with 150 nM Atto565-tubulin growing around frog sperm over time (left). The right side shows the average of 8 structures (mean ± SD).

https://doi.org/10.7554/eLife.31149.016
Figure 3—figure supplement 2
Microtubule dynamics are the same within the entire MCAK-inhibited monopolar spindle.

We compared microtubule dynamics in the inner (radius < 60 m) and outer regions (radius ≥ 60 m) of MCAK-inhibited monopolar spindles. Polymerization velocities were measured by analyzing the speed of EB1-GFP comets in each region (N = 14 for each region, in three different structures from different reactions on the same extract day). Depolymerization velocities were calculated from laser ablation experiments on many different days (N = 55 cuts for the inner region and N = 13 cuts for the outer region). We used single tubulin speckle microscopy to measure the average lifetimes of microtubules (1455 speckles for the inner region and 3418 speckles for the outer region in the same structure). All values are given as mean ± SD.

https://doi.org/10.7554/eLife.31149.017
Figure 4 with 1 supplement
Model for microtubule-stimulated nucleation.

(A) Inactive nucleators (orange circles) are activated around DNA. As active nucleators diffuse (green circles), they can bind and unbind microtubules (red lines). Once bound, they can nucleate a new microtubule with a certain probability. Active nucleators become inactive at a constant rate. (B) Radial microtubule density profiles measured from fluorescent images (mean ± SD, Nmonopoles = 40 (blue), Na-MCAK = 18 (gray)) and corresponding model fit to the MCAK-inhibited and prediction to control monopoles (see Appendix 1). The ratio of the microtubule densities for control and MCAK-inhibited monopoles was determined using structures from the same extract reaction. (C) Parameter-free rescaling of the microtubule density profiles predicted by the model: ρC=ρMexp[(1/M-1/C)x], where ρC, C and ρM, M are the density and length of microtubules for the control and MCAK-inhibited structures, respectively, and x is the distance from the center of the structure. In the graph, blue corresponds to the density profile of control monopoles and gray to the rescaled density profile of MCAK-inhibited monopoles. (D) Data and predictions (orange) for the nucleation profiles of control (blue) and MCAK-inhibited monopoles (gray) up to a global nucleation amplitude, and flux-corrected regular spindles (green) (mean ± SD, Ncontrol = 117 , Na-MCAK = 74, Nspindle = 36 cuts).

https://doi.org/10.7554/eLife.31149.021
Figure 4—figure supplement 1
Example of the spatio-temporal evolution of autocatalytic microtubule nucleation with spatially varying branching nucleation rate kbra, polymerization velocity vp and turnover rate Θ.

The yellow arrows at the origin denote initial microtubule seeds. In the example, a region has a branching rate of kbra=5Θ/3 and another region has vanishing branching rate. Microtubule turnover is shown as a transition from black to dashed black arrows and disappearance. The front velocity vf is denoted in yellow while the plus-end microtubule velocity is denoted in red. As shown in the figure, before the front reaches the boundary where branching is allowed, vp=vf; however, further away vf=0.

https://doi.org/10.7554/eLife.31149.022

Videos

Video 1
EB1-GFP comets in monopolar spindles.

Pre-assembled monopolar spindles (200 μM STLC) were imaged after adding 0.2 µg/ml EB1-GFP. Individual frames were recorded every second with subsequent averaging of 3 frames.

https://doi.org/10.7554/eLife.31149.007
Video 2
Tubulin speckles in a monopolar spindle.

Pre-assembled monopolar spindles (200 µM STLC) were visualized by adding 1 nM purified Atto565 frog tubulin. Images were taken every second with subsequent averaging of 4 frames.

https://doi.org/10.7554/eLife.31149.008
Video 3
Depolymerization wave after a cut in a fluorescently labeled monopole.

200 µM STLC, 150 nM Atto565-tubulin. Images were acquired every 500 ms.

https://doi.org/10.7554/eLife.31149.009
Video 4
Fluorescence intensity loss after a cut in a fluorescently labeled monopole as the depolymerization wave propagates.

200 µM STLC, 150 nM Atto565-tubulin. We calculated the differential intensities for a time interval of 2 s. For purposes of visualization, negative intensity values were set to zero.

https://doi.org/10.7554/eLife.31149.010
Video 5
Tubulin speckles in a monopolar spindle (200 µM STLC) treated with 30 µg/mg anti-MCAK antibodies.

The field of view only shows a quarter of the entire structure. Speckles were created by adding 1 nM purified Atto565 frog tubulin to pre-assembled structures. In every movie, four frames were averaged during the acquisition.

https://doi.org/10.7554/eLife.31149.011
Video 6
EB1-GFP comets in MCAK-inhibited monopoles.

Pre-assembled monopolar spindles (200 µM STLC) were treated with 30 µg/mg anti-MCAK antibodies and imaged after adding 0.2 µg/ml EB1-GFP.

https://doi.org/10.7554/eLife.31149.012
Video 7
Monopole growing against an obstacle.

The microtubule structure is labeled with tubulin Atto565 (red) and EB1-GFP (green) and grows around immobilized frog sperm chromosomes. The bead is attached to the surface and highlighted with an arrow.

https://doi.org/10.7554/eLife.31149.018
Video 8
Ran asters after addition of 30 µM RanQ69L to extract.

Microtubules were labeled with 150 nM Atto565-tubulin. Movie starts 20 min after adding RanQ69L.

https://doi.org/10.7554/eLife.31149.019
Video 9
Growth of pre-existing monopoles after addition of 10 µM RanQ69L.

Monopolar spindles (200 µM STLC, labeled with 150 nM Atto565-tubulin) were imaged on a PLL-g-PEG passivated slide immediately after adding RanQ69L. Scale bar = 20 µm.

https://doi.org/10.7554/eLife.31149.020
Video 10
Depolymerization waves after a cut in a fluorescently labeled spindle.

150 nM Atto565-tubulin. Images were acquired every 500 ms.

https://doi.org/10.7554/eLife.31149.025
Video 11
Fluorescence intensity loss after a cut in a fluorescently labeled spindle as the depolymerization waves propagate.

150 nM Atto565-tubulin. We calculated the differential intensities for a time interval of 2 s. For visualization purposes, negative intensity values were set to zero.

https://doi.org/10.7554/eLife.31149.026

Tables

Table 1
Measurements of microtubule dynamics in spindles and monopoles.
https://doi.org/10.7554/eLife.31149.023
Microtubule dynamicsSpindleMonopoleMCAK-inhibited monopole
Lifetime (s)16 ± 220 ± 260 ± 4
Polymerization velocity (μm/min)23 ± 821 ± 519 ± 5
Depolymerization velocity (μm/min)36 ± 733 ± 646 ± 8
  1. Reference Needleman et al., 2010.

Table 2
Parameters used in the model of autocatalytic microtubule nucleation.
https://doi.org/10.7554/eLife.31149.024
ParameterSymbolValueProcedure
Microtubule turnover rate control monopole (s-1)ΘC0.05 ± 0.01Single molecule microscopy
Microtubule turnover rate MCAK-inhibited monopole (s-1)ΘM0.016 ± 0.001Single molecule microscopy
Microtubule length control monopole (μm)C8.0 ± 0.3Laser ablation
Microtubule length MCAK-inhibited monopole (μm)M24 ± 4Laser ablation
Length scale gradient unbound nucleators (μm)u24.9 ± 0.5Fit to the model
Branching parameter (dimensionless)α2.38 ± 0.01Fit to the model
Microtubule density at the center of the monopole (a.u.)ρ(0)4058 ± 64Fit to the model

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