The right panel shows the 1159 protein change profiles with the strongest values, grouped by similarity. In this visualization, rows represent the protein change profiles for individual proteins, whereas columns represent feature measurements, with each set of 50 measurements corresponding to an image screen. Positive values are blue, whereas negative values are yellow, with the intensity of color corresponding to the magnitude of the value, as shown in the color bar. Grey values are missing data. The headers indicate the image screens that sets of features correspond to, abbreviated using WT to indicate the untreated wild-type screens, ΔRPD3 for the rpd3Δ replicates, RAP for time points of the rapamycin treatment, HU for time points of the hydroxyurea treatment, αF for time points of the α-factor treatment, and ΔIKI for the iki3Δ replicates. The dendrogram depth shows similarity between connected protein profiles or groups of profiles. We highlight examples of strong patterns of protein change profiles in yellow, with some clusters that we have annotations for labelled from A to T, with labels and enrichments for some clusters presented in Table 1. In the four boxes on the left, we show examples of localization changes found in our clusters of protein change profiles. The images are representative cropped micrographs of yeast cells, where the protein named in the top left corner of each box has been tagged with GFP (shown as the green channel). The blue lines in the images show the boundaries drawn between cells by our single-cell segmentation algorithm, the small white circles between cells indicate mother-bud relations, and the white meshed regions indicate areas that have been ignored by our image analysis because they are likely to be artifacts or mis-segmented cells.