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LPCAT1 controls phosphate homeostasis in a zinc-dependent manner

  1. Mushtak Kisko
  2. Nadia Bouain
  3. Alaeddine Safi
  4. Anna Medici
  5. Robert C Akkers
  6. David Secco
  7. Gilles Fouret
  8. Gabriel Krouk
  9. Mark GM Aarts
  10. Wolfgang Busch
  11. Hatem Rouached  Is a corresponding author
  1. INRA, France
  2. Wageningen University, Netherlands
  3. CNRS, France
  4. Salk Institute for Biological Studies, United States
Research Article
  • Cited 8
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Cite this article as: eLife 2018;7:e32077 doi: 10.7554/eLife.32077

Abstract

All living organisms require a variety of essential elements for their basic biological functions. While the homeostasis of nutrients is highly intertwined, the molecular and genetic mechanisms of these dependencies remains poorly understood. Here, we report a discovery of a molecular pathway that control phosphate (Pi) accumulation plants in Zn deficiency. Using genome-wide association studies we first identified allelic variation of the Lyso-PhosphatidylCholine (PC) AcylTransferase 1 (LPCAT1) gene as the key determinant of shoot Pi accumulation under Zn deficiency. We then show that regulatory variation at the LPCAT1 locus contributes significantly to this natural variation and we further demonstrate that the regulation of LPCAT1 expression involves bZIP23 TF, for which we identified a new binding site sequence. Finally, we show that in Zn deficient conditions loss of function of LPCAT1 increases the phospholipid Lyso-PhosphatidylCholine/PhosphatidylCholine ratio, the expression of the Pi transporter PHT1;1, and that this leads to shoot Pi accumulation.

Article and author information

Author details

  1. Mushtak Kisko

    Biochimie et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Nadia Bouain

    Biochimie et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Alaeddine Safi

    Biochime et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1532-5708
  4. Anna Medici

    Biochimie et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Robert C Akkers

    Laboratory of Genetics, Wageningen University, Wageningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  6. David Secco

    Biochimie et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  7. Gilles Fouret

    Unité Mixte de Recherche 866, INRA, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  8. Gabriel Krouk

    INSB, CNRS, Montpellier, France
    Competing interests
    The authors declare that no competing interests exist.
  9. Mark GM Aarts

    Laboratory of Genetics, Wageningen University, Wageningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  10. Wolfgang Busch

    Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States
    Competing interests
    The authors declare that no competing interests exist.
  11. Hatem Rouached

    Biochimie et Physiologie Moléculaire des Plantes, INRA, Montpellier, France
    For correspondence
    hatem.rouached@supagro.inra.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7751-0477

Funding

Institut National de la Recherche Agronomique

  • Hatem Rouached

The Austrian Academy of Sciences through Gregor Mendel Institute

  • Wolfgang Busch

The Netherland Genome Initiative ZonMW Horizon program Zenith (no. 40-41009-98-11084)

  • Mark GM Aarts

Iraq Government Doctoral Fellowship

  • Mushtak Kisko

Région Languedoc-Roussillon: Chercheurd'Avenir/Projet Cofinancé par le Fonds Européen de Développement Regional

  • Hatem Rouached

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Maria J Harrison, Boyce Thompson Institute for Plant Research, United States

Publication history

  1. Received: September 17, 2017
  2. Accepted: February 15, 2018
  3. Accepted Manuscript published: February 17, 2018 (version 1)
  4. Version of Record published: February 26, 2018 (version 2)
  5. Version of Record updated: February 28, 2018 (version 3)

Copyright

© 2018, Kisko et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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