(A) Schematic of Drosophila larval central nervous system showing brain lobes and ventral nerve cord. In this study we analyzed neuroblasts (green circles) from the brain lobes outside of the optic lobes. Neuroblasts were stained for Dlg, aPKC, the centrosome marker centrosomin (cnn) to measure spindle angle, and the polarized neural fate determinant Miranda (Mira), after dissection from animals with the following genotypes (B) Wild-type (C) dlgm52 null mutant (D) dlgm52 Worniu-Gal4 >UAS WT Dlg (E) dlgm52 Worniu-Gal4 >UAS Dlg with a phosphomimetic mutation at site S662 (E') the same genotype as in panel D but showing the variability in Mira polarization we observed. For each condition, a polar histogram with the measured spindle angles is shown along with a p-value calculated by comparison with the wild-type condition using the K-S test. (F) Quantification of neuroblasts exhibiting polarized Mira for the conditions shown in panels B-E'. Individual measurements are included in comma separated value format in Figure 3—source data 1.