BAC GFP-Rab5 cells were labeled with 100 nM Mito-Red at 37°C for 30 min. Cells were acquired prior to laser irradiation as controls (Untreated) (A), (C), (E), and (G). Same cells were photoirradiated as before, fixed after 60 min post-laser treatment, and immunostained with specific antibodies against LC3 (B), Lamp1 (D), Parkin (F), and Bax (H). Inset images show the area before and after laser treatment. (I) Colocalization analysis of Mito-Red to Rab5, LC3, Lamp1, Parkin, and Bax, in untreated vs laser-induced conditions (n = 3). Untreated cells correspond to cells outside of the laser treated area. Error bars represent SEMs. p Values based on two-tailed t-tests. Scale bars, 10 μm.