Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex
- Fudan University, China
Abstract
Eye opening, a natural and timed event during animal development, influences cortical circuit assembly and maturation; yet, little is known about its precise effect on inhibitory synaptic connections. Here we show that coinciding with eye opening, the strength of unitary inhibitory postsynaptic currents (uIPSCs) from somatostatin-expressing interneurons (Sst-INs) to nearby excitatory neurons, but not interneurons, sharply decreases in layer 2/3 of the mouse visual cortex. In contrast, the strength of uIPSCs from fast-spiking interneurons (FS-INs) to excitatory neurons significantly increases during eye opening. More importantly, these developmental changes can be prevented by dark rearing or binocular lid suture, and reproduced by the artificial opening of sutured lids. Mechanistically, this differential maturation of synaptic transmission is accompanied by a significant change in the postsynaptic quantal size. Together, our study reveals a differential regulation in GABAergic circuits in the cortex driven by eye opening may be crucial for cortical maturation and function.
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Author details
Funding
National Key Research and Development Program of China (2016YFA0100802)
- Yinghui Fu
Ministry of Education of the People's Republic of China (20130071110065)
- Yong-Chun Yu
Science and Technology Commission of Shanghai Municipality (No.15JC1400102)
- Yong-Chun Yu
Science and Technology Commission of Shanghai Municipality (15XD1500700)
- Yong-Chun Yu
National Natural Science Foundation of China (31200816)
- Yinghui Fu
National Natural Science Foundation of China (31271157)
- Yong-Chun Yu
National Natural Science Foundation of China (31471036)
- Yong-Chun Yu
National Natural Science Foundation of China (31629004)
- Yong-Chun Yu
National Natural Science Foundation of China (31421091)
- Yong-Chun Yu
National Natural Science Foundation of China (91332110)
- Yong-Chun Yu
Ministry of Science and Technology of the People's Republic of China (2014CB942800)
- Yong-Chun Yu
Ministry of Science and Technology of the People's Republic of China (2012CB966303)
- Yong-Chun Yu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All surgical and experimental procedures were in accordance with the protocols approved by the Committee on the Ethics of Animal Experiments of Fudan University Shanghai Medical College (permit number: 20110307-049). All surgery was performed under isoflurane anesthesia and ethanol disinfection to minimize suffering.
Copyright
© 2017, Guan et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex
eLife 6:e32337.
https://doi.org/10.7554/eLife.32337
