Architecture of the human mTORC2 core complex
- University of Basel, Switzerland
- ETH Zürich, Switzerland
Abstract
The mammalian target of rapamycin (mTOR) is a key protein kinase controlling cellular metabolism and growth. It is part of the two structurally and functionally distinct multiprotein complexes mTORC1 and mTORC2. Dysregulation of mTOR occurs in diabetes, cancer and neurological disease. We report the architecture of human mTORC2 at intermediate resolution, revealing a conserved binding site for accessory proteins on mTOR and explaining the structural basis for the rapamycin insensitivity of the complex.
Data availability
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Human mTORC2 Core Complex (C2)Publicly available at the EBI EM-3927.
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Human mTORC2 core complex accessory factorsPublicly available at the EBI EM-3928.
Article and author information
Author details
Funding
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (51NF40_141735_NCCR)
- Nenad Ban
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (310030_159492)
- Michael N Hall
European Research Council (609883)
- Michael N Hall
Sir Henry Dale Fellowship (206212/Z/17/Z)
- Christopher H S Aylett
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (138262)
- Nenad Ban
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (159696)
- Timm Maier
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (310030B_163478)
- Nenad Ban
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Stuttfeld et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Architecture of the human mTORC2 core complex
eLife 7:e33101.
https://doi.org/10.7554/eLife.33101
Further reading
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- Biochemistry and Chemical Biology
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