1. Structural Biology and Molecular Biophysics
Download icon

Architecture of the human mTORC2 core complex

  1. Edward Stuttfeld
  2. Christopher H S Aylett
  3. Stefan Imseng
  4. Daniel Boehringer
  5. Alain Scaiola
  6. Evelyn Sauer
  7. Michael N Hall  Is a corresponding author
  8. Timm Maier  Is a corresponding author
  9. Nenad Ban  Is a corresponding author
  1. University of Basel, Switzerland
  2. ETH Zürich, Switzerland
Short Report
  • Cited 3
  • Views 2,996
  • Annotations
Cite as: eLife 2018;7:e33101 doi: 10.7554/eLife.33101

Abstract

The mammalian target of rapamycin (mTOR) is a key protein kinase controlling cellular metabolism and growth. It is part of the two structurally and functionally distinct multiprotein complexes mTORC1 and mTORC2. Dysregulation of mTOR occurs in diabetes, cancer and neurological disease. We report the architecture of human mTORC2 at intermediate resolution, revealing a conserved binding site for accessory proteins on mTOR and explaining the structural basis for the rapamycin insensitivity of the complex.

Article and author information

Author details

  1. Edward Stuttfeld

    Biozentrum, University of Basel, Basel, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-3932-9076
  2. Christopher H S Aylett

    Institute for Molecular Biology and Biophysics, ETH Zürich, Zürich, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  3. Stefan Imseng

    Biozentrum, University of Basel, Basel, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  4. Daniel Boehringer

    Institute for Molecular Biology and Biophysics, ETH Zürich, Zürich, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  5. Alain Scaiola

    Institute for Molecular Biology and Biophysics, ETH Zürich, Zürich, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  6. Evelyn Sauer

    Biozentrum, University of Basel, Basel, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  7. Michael N Hall

    Biozentrum, University of Basel, Basel, Switzerland
    For correspondence
    m.hall@unibas.ch
    Competing interests
    The authors declare that no competing interests exist.
  8. Timm Maier

    Biozentrum, University of Basel, Basel, Switzerland
    For correspondence
    timm.maier@unibas.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-7459-1363
  9. Nenad Ban

    Institute for Molecular Biology and Biophysics, ETH Zürich, Zürich, Switzerland
    For correspondence
    ban@mol.biol.ethz.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-9527-210X

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (51NF40_141735_NCCR)

  • Nenad Ban

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (310030_159492)

  • Michael N Hall

European Research Council (609883)

  • Michael N Hall

Sir Henry Dale Fellowship (206212/Z/17/Z)

  • Christopher H S Aylett

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (138262)

  • Nenad Ban

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (159696)

  • Timm Maier

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (310030B_163478)

  • Nenad Ban

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Nahum Sonenberg, Reviewing Editor, McGill University, Canada

Publication history

  1. Received: October 25, 2017
  2. Accepted: February 8, 2018
  3. Accepted Manuscript published: February 9, 2018 (version 1)
  4. Version of Record published: March 5, 2018 (version 2)

Copyright

© 2018, Stuttfeld et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,996
    Page views
  • 698
    Downloads
  • 3
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics
    Kathryn E Livingston et al.
    Research Article
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics
    Petr Popov et al.
    Research Article