strain, strain background (C57BL/6J) | C57BL/6J; WT; wild-type | The Jackson Laboratory | Jackson Stock #: 000664; RRID:IMSR_JAX:000664 | |
strain, strain background (B6.129S6- S1pr3tm1Rlp/Mmnc) | S1PR3 KO; S1pr3-/- | MMRRC Repository; https://www.ncbi.nlm.nih.gov/pubmed/15138255; PMID: 15138255 | B6.129S6-S1pr3tm1Rlp/ Mmnc; MMRRC Stock #: 012038-UNC; RRID: MMRRC_012038-UNC | |
strain, strain background (B6.Cg- S1pr3tm1.1Hrose/J) | S1pr3-mCherry; S1pr3mcherry/+ | The Jackson Laboratory | B6.Cg-S1pr3tm1.1Hrose/J; Jackson Stock #: 028624; RRID:IMSR_JAX:028624 | |
antibody (Living Colors DsRed Rabbit Polyclonal Antibody) | Rabbit anti-DsRed | Clontech | RRID:AB_10013483; Cat # 632496 | |
antibody (Chicken polyclonal to Neurofilament heavy polypeptide) | Chicken anti-NefH | Abcam | RRID:AB_304560; Cat # ab4680 | |
antibody (Chicken polyclonal to beta III Tubulin) | Chicken anti-β-tubulin III | Abcam | RRID:AB_10899689; Cat # ab107216 | |
antibody (Mouse monoclonal [13C4/I3 C4] to PGP9.5) | Mouse anti-PGP9.5 | Abcam | RRID:AB_306343; Cat # ab8189 | |
antibody (Rabbit polyclonal to EDG3) | Rabbit anti-S1PR3 | Abcam | RRID:AB_732070; Cat # ab38324 | |
antibody (Mouse monoclonal to NF200) | Mouse anti-NF200 | Sigma-Aldrich | RRID:AB_260781; Cat # N5389 | |
antibody (Chicken polyclonal to Peripherin) | Chicken anti-Peripherin | Abcam | RRID:AB_777207; Cat # ab39374 | |
antibody (Goat Anti-Mouse IgG H and L Alexa Fluor 488) | Goat anti-Mouse Alexa 488 | Abcam | RRID:AB_2688012; Cat # ab150117 | |
antibody (Goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa Fluor 488) | Goat anti-Chicken Alexa 488 | ThermoFisher Scientific | RRID:AB_2534096; Cat # A-11039 | |
antibody (Goat anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 594) | Goat anti-Rabbit Alexa 594 | Invitrogen | RRID:AB_2556545; Cat # R37117 | |
sequence-based reagent | S1pr3 Type I Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB1-19668-VC | |
sequence-based reagent | Scn1a Type 6 Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB6-18173-VC | |
sequence-based reagent | Npy2r Type 6 Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB6-3197254-VC | |
sequence-based reagent | Piezo2 Type 6 Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB6-18046-VC | |
sequence-based reagent | Trpv1 Type 6 Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB6-18246-VC | |
sequence-based reagent | Trpa1 Type 6 Probe | ThermoFisher Scientific; Affymetrix | Assay ID: VB6-16610-VC | |
commercial assay or kit (ViewRNA ISH Tissue Assay Kit (2-plex)) | ViewRNA ISH Tissue Assay Kit | ThermoFisher Scientific; Affymetrix | Cat # QVT0012 | |
chemical compound, drug (Sphingosine-1-phosphate) | Sphingosine 1-phosphate; S1P | Tocris Bioscience; Avanti Polar Lipids | CAS 26993-30-6; Cat # 1370; Cat # 860641 | |
chemical compound, drug (TY 521256) | TY 52156 | Tocris Bioscience | CAS 934369-14-9; Cat # 5328 | |
chemical compound, drug (SKI II) | SKI II | Tocris Bioscience | CAS 312636-16-1; Cat # 2097 | |
chemical compound, drug (Histamine dihydrochloride) | Histamine | Sigma-Aldrich | CAS 56-92-8; Cat # H7250 | |
chemical compound, drug (Chloroquine diphosphate) | Chloroquine | Sigma-Aldrich | CAS 50-63-5; Cat # C6628 | |
chemical compound, drug (E-Capsaicin) | Capsaicin | Tocris Bioscience | CAS 404-86-4; Cat # 0462 | |
chemical compound, drug (Dimethyl sulfoxide) | DMSO | Sigma-Aldrich | Cat # 8418–100 mL | |
chemical compound, drug (Methanol) | Methanol | Sigma-Aldrich | CAS 67-56-1; Cat # 34860 | |
chemical compound, drug (Linopirdine dihydrochloride) | Linopirdine | Tocris Bioscience | CAS 113168-57-3; Cat # 1999 | |
chemical compound, drug (XE 991 dihydrochloride) | XE 991 | Tocris Bioscience | CAS 122955-13-9; Cat # 2000 | |
chemical compound, drug (W146) | W146 | Tocris Bioscience | CAS 909725-61-7; Cat # 3602 | |
chemical compound, drug (Freund's Adjuvant, Complete) | Complete Freund's Adjuvant; CFA | Sigma-Aldrich | Cat # F5881 | |
chemical compound, drug (Formaldehyde, 16%, methanol free, Ultra Pure) | Paraformaldehyde; PFA | Polysciences, Inc. | Cat # 18814–10 | |
chemical compound, drug (Tissue Tek Optimal cutting temperature compound (OCT)) | OCT | Sakura Finetek USA | Cat # 4583 | |
chemical compound, drug (Triton X-100 solution) | Triton X-100 | BioUltra | CAS 9002-93-1; Cat # 93443 | |
chemical compound, drug (Phosphate-buffered saline (PBS), pH 7.4) | PBS | Gibco | Cat # 10010023 | |
chemical compound, drug (Benzyl benzoate) | Benzyl benzoate | Sigma-Aldrich | CAS 120-51-4; Cat # B6630 | |
chemical compound, drug (Benzyl alcohol) | Benzyl alcohol | Sigma-Aldrich | CAS 100-51-6; Cat # 305197 | |
chemical compound, drug (Sucrose) | Sucrose | Sigma-Aldrich | CAS 57-50-1; Cat # S0389 | |
chemical compound, drug (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation) | Aqua | ThermoFisher Scientific | Cat # L34957 | |
chemical compound, drug (Isoflurane, USP) | Isoflurane | Piramal | CAS 26675-46-7 | |
chemical compound, drug (4',6-Diamidino-2- Phenylindole, Dihydrochloride) | DAPI | ThermoFisher Scientific | CAS 28718-90-3; Cat # 1306 | |
chemical compound, drug (Fluoromount-G, with DAPI) | Fluoromount-G, with DAPI | ThermoFisher Scientific | Cat # 00-4959-52 | |
antibody (CD117 (c-Kit) Monoclonal Antibody (2B8), Biotin) | c-Kit-Biotin | eBioscience | RRID:AB_466569; Cat # 13-1171-82 | |
antibody (FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE, eBioscience) | FceRI-PE | eBioscience | RRID:AB_10804885; Cat # 12-5899-42 | |
antibody (CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience) | CD49b-PECy7 | eBioscience | RRID:AB_469667; Cat # 25-5971-82 | |
antibody (Anti-Siglec-F-APC, mouse (clone: REA798)) | SiglecF-APC | Miltenyi Biotech | RRID:AB_2653441; Cat # 130-112-333 | |
antibody (Streptavidin FITC) | SA-FITC | eBioscience | RRID:AB_11431787; Cat # 11-4317-87 | |
antibody (Ly-6C Monoclonal Antibody (HK1.4), PerCP- Cyanine5.5, eBioscience) | Ly6C-PerCP | eBioscience | RRID:AB_1518762; Cat # 45-5932-82 | |
antibody (Pacific Blue anti- mouse/human CD11b Antibody) | CD11b-PB | BioLegend | RRID:AB_755985; Cat # 101223 | |
antibody (Brilliant Violet 785 anti-mouse Ly-6G Antibody) | Ly6G-BV785 | BioLegend | RRID:AB_2566317; Cat # 127645 | |
antibody (CD45.2 Monoclonal Antibody (104), Alexa Fluor 700, eBioscience) | CD45.2-AF700 | eBioscience | RRID:AB_657752; Cat # 56-0454-82 | |
software, algorithm (Igor Pro version 6.3) | IgorPro | WaveMetrics | https://www.wavemetrics.com/order/order_igordownloads6.htm | |
software, algorithm (Microsoft Excel 2011) | Microsoft Excel | Microsoft | https://www.microsoft.com/en-us/store/d/excel-2016-for-mac/ | |
software, algorithm (pClamp 10) | pClamp | Axon | http://mdc.custhelp.com/app/answers/detail/a_id/18779/~/axon%E2%84%A2-pclamp%E2%84%A2-10-electrophysiology-data-acquisition-%26-analysis-software | |
software, algorithm (MetaFluor 7.8) | MetaFluor | Molecular Devices | https://www.moleculardevices.com/systems/metamorph-research-imaging/metafluor-fluorescence-ratio-imaging-software | |
software, algorithm (MATLAB) | MATLAB | MathWorks | https://www.mathworks.com/downloads/ | |
software, algorithm (FIJI) | FIJI | NIH | https://imagej.net/Fiji/Downloads | |
software, algorithm (LabChart Software) | LabChart Software | AD Instruments | https://www.adinstruments.com/products/labchart | |
software, algorithm (Graphpad Prism 7) | Graphpad Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ | |
software, algorithm (FlowJo 10.4.2) | FlowJo | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads | |
software, algorithm (custom) | custom-made software in MATLAB | this paper | NA | https://github.com/buh2003/SpikeSortingPCA_DBSCAN (Hoffman, 2018; copy archived at https://github.com/elifesciences-publications/SpikeSortingPCA_DBSCAN) |
other (Bovine serum albumin, cold ethanol fraction, pH 5.2,≥96%) | BSA | Sigma-Aldrich | CAS 9048-46-8; Cat # A4503 | |
other (Isolectin B4 (Bandeireia simplicifolia), FITC-conjugate) | IB4-FITC; IB4 | Enzo Life Sciences | Cat # ALX-650–001F- MC05 | |
other (Normal Goat Serum) | NGS | Abcam | Cat # ab7481 | |
peptide, recombinant protein (δ-theraphototoxin- Hm1a) | Hm1a | other; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919188/; PMID: 4919188 | NA | obtained from the laboratory of David Julius (UCSF) |