Source data files have been provided for Figure 1G&H, Figure 2A&B, Figure 1-Figure supplement1A&B, Figure 1-Figure supplement 2A&B, Figure 1-Figure supplement 3B&C, Figure 2-Figure supplement2A&C, and Figure 2-Figure supplement 6. Due to their large size (>300GB), image datasets will be available from the corresponding author on reasonable request. Full sequence information for tCRMod-GGS5 (Plasmid #111760), tCRMod-GGS7 (Plasmid #111761), tCRMod-GGS9 (Plasmid#111762), VinTS-tCRMod-GGS5 (Plasmid #111763), VinTS-tCRMod-GGS7 (Plasmid #111764), andVinTS-tCRMod-GGS9 (Plasmid #111765) is available at Addgene (http://www.addgene.org). All software developed in the current study is publicly available on GitLab (https://gitlab.oit.duke.edu/HoffmanLab-Public), including code to perform image preprocessing (‘image-preprocessing’ repository), FRET analysis and FA segmentation (‘fret-analysis’ repository), fluorometric FRET analysis(‘fluorimetry-fret’ repository), calibration model simulations (‘tsmod-calibration-model’ repository), and FA structural model simulations (‘FA-structural-model’ repository).
tCRMod-GGSGGS9Publicly available at Addgene (iD 111762).
tCRMod-GGSGGS7Publicly available at Addgene (ID 111761).
tCRMod-GGSGGS5Publicly available at Addgene (ID 111760).
tCRMod-GGSGGS5Publicly available at Addgene (ID 111763).
tCRMod-GGSGGS7Publicly available at Addgene (ID 111764).
tCRMod-GGSGGS9Publicly available at Addgene (ID 111765).
Supplementary file 1
Supplementary file 2
Supplementary file 3
Supplementary file 4
Transparent reporting form