(A) Running average plot of mCG methylation levels on DBS upregulated genes (shown in red; FDR < 0.05,>2 fold increase in expression), DBS downregulated genes (shown in blue; FDR < 0.05,>2 fold decrease in expression), and in genes whose expression is unchanged by DBS (shown in black). The left panel shows the mCG methylation of these genes observed in sham samples. The right panel shows the mCG methylation of these categories of genes observed in DBS samples. (B) Genomic locations of differentially methylated regions (DMRs) with a greater than 50% change in methylation following DBS. Locations evaluated: intergenic, introns, exons, and promoters. The percentage of DMRs falling into each location type was divided by the percentage of the genome comprised of that location type to generate a fold enrichment score for DMR locations relative to the genomic distribution. Dashed line at y = 1 indicates the genomic values. *p<0.05, **p<0.01. (C) Fosl2 mCG methylation and RNA-sequencing tracks from representative WT sham (shown in black) and WT DBS (shown in red) samples. The annotated gene is shown in blue, and the mCG level in the promoter region is highlighted in the yellow boxes. Figure 4—figure supplement 1 shows mCH distribution at promoters and gene bodies in DBS-regulated genes, and it also shows genomic localization of DMRs with a greater than 50% change in methylation following DBS. The DMR source data, including p-values, percent change and genomic localization is provided in Figure 4—source data 1.