(A) Top left, overview of the double translocated ribosome complex with eRF1* with the L1 stalk region highlighted. Main view, L1 stalk in the double translocated complex (cyan) is displaced from the position acquired in a complex with canonical tRNAs (grey) with a magnitude of approximately 30 Å. This displacement is similar to the one reported for the pre-translocated complex with eEF2 and a non-hydrolyzable GTP analog (orange)(Murray et al., 2016). (B) Conformational transition observed in CrPV-IRES upon back-swiveling of the 40S head. Left, due to a swiveled 40S head configuration in the double translocated complex with eEF2, SL-IV and SL-V remain solvent exposed, as in the single translocated complex (Muhs et al., 2015), and detached from the ribosomal protein eS25 (green). Right, once the head of the 40S relocates to its non-swiveled position, CrPV-IRES acquires a new conformation involving a new interaction with eS25 (green). (C) Scheme showing the secondary structure of CrPV-IRES in the pre-translocated state (left), after a single translocation (center) and after the double translocation (right). Arrows indicate the repositioning of PKII and PKIII as well as the new interaction established with ribosomal protein eS25. On the right, close up views of the final unsharped map obtained for this reconstruction for the regions indicated by circles.