Auditory experience controls the maturation of song discrimination and sexual response in Drosophila
- Nagoya University, Japan
Figures
Figure 1
Social interaction shapes the preference to the song.
Chaining response of naïve male flies that were housed in different experimental conditions, grouped without wings (A), grouped with intact wings (B), and single-reared with intact wings (C). The …
Figure 2
Fine-tuned song response of males after the training.
(A) Protocol for the training and test sessions. (B) Setup for the training and chaining test. In the training session, single-housed male flies were exposed to a training song for the first 6 days …
Figure 3
Playback of pulse song promotes copulation in wild-type fly pairs.
(A) Setup for song-induced copulation test. (B) Cumulative copulation rate and copulation latency with playback of artificial pulse songs of different inter-pulse interval (IPI). Copulation latency …
Figure 4
Fine-tuned song response of females after the training.
(A, B) Cumulative copulation rate in the conspecific song test (A) or heterospecific song test (B) after training females. Naïve group (no sound during training) and experienced groups (trained with …
Figure 5 with 1 supplement
Involvement of Gad1 in the experience-dependent song preference in females.
(A, B) Cumulative copulation rate in the conspecific song test (A) or heterospecific song test (B) after training Gad1 knockdown (left) and control (right) females. Naïve group (no sound training) …
Figure 5—figure supplement 1
Male Gad1 knockdown flies responded normally to conspecific courtship song.
The time-courses of the chain index in response to playback of conspecific song in Gad1 knockdown group (red, Gad1-GAL4/+; UAS-Gad1 RNAi/+) and control group (blue, Gad1-GAL4/+; +/+) are shown. The …
Figure 6
Rdl receptors in pC1 neurons modulate the experience-dependent song preference in females.
(A, B) Cumulative copulation rate in the conspecific song test (A) or heterospecific song test (B) after training Rdl knockdown (left) and control (right) females. Naïve group (no sound training) …
Figure 7
A model for experience-dependent tuning of IPI perception in Drosophila.
https://doi.org/10.7554/eLife.34348.010Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| gene (Drosophila melanogaster) | Rdl | NA | FLYB: FBgn0004244 | |
| gene (Drosophila melanogaster) | GAD1 | NA | FLYB: FBgn0004516 | |
| strain, strain background (Drosophila melanogaster) | Canton-S | other | gift from K. Ito | |
| strain, strain background (Drosophila melanogaster) | Gad1-GAL4 | PMID: 12408848 | gift from K. Ito | |
| strain, strain background (Drosophila melanogaster) | UAS-Gad1 RNAi | Vienna Drosophila Resource Center | VDRC ID: 32344; RRID: Fly-Base_FBst0459538 | |
| strain, strain background (Drosophila melanogaster) | w1118 | Vienna Drosophila Resource Center | VDRC ID: 60000 | |
| strain, strain background (Drosophila melanogaster) | UAS-Rdl RNAi | Bloomington Drosophila Stock Center | BDRC: 52903; RRID: BDSC_52903 | |
| strain, strain background (Drosophila melanogaster) | TRiP RNAi | Bloomington Drosophila Stock Center | BDRC: 36304; RRID: BDSC_36304 | |
| strain, strain background (Drosophila melanogaster) | tubP>GAL80>; NP2631-GAL4/CyO; dsxFLP/TM2 | PMID: 27185554 | gift from D. Yamamoto | |
| software, algorithm | ChaIN (ver. 3) | PMID: 28701929 |
Additional files
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Supplementary file 1
Genotypes.
- https://doi.org/10.7554/eLife.34348.011
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Supplementary file 2
Statistical results.
The detailed statistical results in each figure are listed. N.S., not significant, p>0.05; *p<0.05; **p<0.01; ***p<0.001.
- https://doi.org/10.7554/eLife.34348.012
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Transparent reporting form
- https://doi.org/10.7554/eLife.34348.013
