Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb
- Humboldt- Universität zu Berlin, Germany
- Institut Pasteur, France
- Max Planck Institute of Molecular Cell Biology and Genetics, Germany
- Janelia Farm Research Campus, Howard Hughes Medical Institute, United States
- Max Delbrück Center for Molecular Medicine, Germany
- Max Planck Institute of Cell Biology and Genetics, Germany
- Cited 5
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Abstract
During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.
Article and author information
Author details
Anastasios Pavlopoulos
Funding
Howard Hughes Medical Institute
- Evangelia Stamataki
- Léo Guignard
- Philipp J Keller
- Anastasios Pavlopoulos
European Commission Marie Sklodowska-Curie Actions (FP7-IEF 302235)
- Anastasios Pavlopoulos
Max-Planck-Institute of Molecular Cell Biology and Genetics
- Tobias Pietzsch
- Benjamin Harich
- Pavel Tomancak
European Research Council (260746)
- Tobias Pietzsch
- Pavel Tomancak
Einstein Stiftung Berlin (A-2012_114)
- Carsten Wolff
Institut Pasteur
- Jean-Yves Tinevez
- Spencer Shorte
Agence Nationale de la Recherche
- Jean-Yves Tinevez
- Spencer Shorte
Helmholtz-Gemeinschaft
- Stephan Preibisch
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Alejandro Sánchez Alvarado, Stowers Institute for Medical Research, United States
Publication history
- Received: December 15, 2017
- Accepted: March 26, 2018
- Accepted Manuscript published: March 29, 2018 (version 1)
- Version of Record published: May 1, 2018 (version 2)
Copyright
© 2018, Wolff et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Further reading
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- Developmental Biology
- Stem Cells and Regenerative Medicine
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- Developmental Biology
- Evolutionary Biology
