Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly Megaselia abdita
- Ruprecht Karls University, Germany
- Ruprecht Karls Universität, Germany
- European Molecular Biology Laboratory, Germany
Abstract
Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue spreading are not well understood. Here we analyze spreading of the extraembryonic serosa in the scuttle fly Megaselia abdita. The serosa forms from a columnar blastoderm anlage, becomes a squamous epithelium, and eventually spreads over the embryo proper. We describe the dynamics of this process in long-term, whole-embryo time-lapse recordings, demonstrating that free serosa spreading is preceded by a prolonged pause in tissue expansion. Closer examination of this pause reveals mechanical coupling to the underlying yolk sac, which is later released. We find mechanical coupling prolonged and serosa spreading impaired after knockdown of M. abdita Matrix metalloprotease 1. We conclude that tissue-tissue interactions provide a critical functional element to constrain spreading epithelia.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Custom Matlab functions are available via GitHub (https://github.com/lemkelab/SPIMaging).
Article and author information
Author details
Funding
Deutsche Forschungsgemeinschaft (LE 2787/1-1)
- Francesca Caroti
- Paula González Avalos
- Maike Wosch
- Steffen Lemke
HSFP (RGY0082/2015)
- Everardo González Avalos
- Viola Noeske
- Paula González Avalos
EMBL International PhD Programme
- Dimitri Kromm
Center of Modeling and Simulation in the Biosciences , University of Heidelberg
- Lars Hufnagel
HBIGS predoctoral fellowship
- Lucas Schütz
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Caroti et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly Megaselia abdita
eLife 7:e34616.
https://doi.org/10.7554/eLife.34616
