Regenerating hair cells in human vestibular sensory epithelia

Abstract
Data availability
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Abstract

Human vestibular sensory epithelia in explant culture were incubated in gentamicin to ablate hair cells. Subsequent transduction of supporting cells with ATOH1 using an Ad-2 viral vector resulted in generation of highly significant numbers of cells expressing the hair cell marker protein myosin VIIa. Cells expressing myosin VIIa were also generated after blocking the Notch signalling pathway with TAPI-1 but less efficiently. Transcriptomic analysis following ATOH1 transduction confirmed up-regulation of 335 putative hair cell marker genes, including several downstream targets of ATOH1. Morphological analysis revealed numerous cells bearing dense clusters of microvilli at the apical surfaces which showed some hair cell-like characteristics confirming a degree of conversion of supporting cells. However, no cells bore organised hair bundles and several expected hair cell markers genes were not expressed suggesting incomplete differentiation. Nevertheless, the results show a potential to induce conversion of supporting cells in the vestibular sensory tissues of humans.

Data availability

All sequencing data from all of these samples have been deposited in NCBI GEO (accession number: GSE109320)

The following data sets were generated

1. Forge A
2. Taylor RR
3. Filia A
4. Paredes U
5. Asai Y
6. Holt JR
7. Lovett M
(2018) Regenerating hair cells in human vestibular sensory epithelia
Publicly available at the NCBI Gene Expression Omnibus (accession no. GSE109320).

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE109320

Article and author information

Author details

Ruth Rebecca Taylor

UCL Ear Institute, University College London, London, United Kingdom

For correspondence
Ruth.r.taylor@ucl.ac.uk

Competing interests
The authors declare that no competing interests exist.
Anastasia Filia

National Heart and Lung Institute, Imperial College London, London, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Ursula Paredes

UCL Ear Institute, University College London, London, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Yukako Asai

FM Kirby Neurobiology Center, Harvard Medical School, Boston, United States

Competing interests
The authors declare that no competing interests exist.
Jeffrey R Holt

FM Kirby Neurobiology Center, Harvard Medical School, Boston, United States

Competing interests
The authors declare that no competing interests exist.
Michael Lovett

National Heart and Lung Institute, Imperial College London, London, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Andrew Forge

UCL Ear Institute, University College London, London, United Kingdom

For correspondence
a.forge@ucl.ac.uk

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-0995-0219

Funding

Medical Research Council (Project grant,G1000068)

Ruth Rebecca Taylor
Andrew Forge

Dunhill Medical Trust (Project grant R395/1114)

Andrew Forge

Rosetrees Trust (Project grant M58-F1)

Andrew Forge

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Human subjects: Ethical approval from NHS Health Research Authority, NRES Committee London -Surrey Borders. REC reference 11/LO/0475; IRAS project ID 73422. Tissue was collected anonymously with informed consent of the patient for tissue harvesting and publication of the results of the study.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.