1. Microbiology and Infectious Disease
  2. Plant Biology
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The plant specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity

  1. Jun Qin
  2. Kailun Wang
  3. Lifan Sun
  4. Haiying Xing
  5. Sheng Wang
  6. Lin Li
  7. She Chen
  8. Hui-Shan Guo  Is a corresponding author
  9. Jie Zhang  Is a corresponding author
  1. Chinese Academy of Sciences, China
  2. National Institute of Biological Sciences, China
Research Article
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Cite as: eLife 2018;7:e34902 doi: 10.7554/eLife.34902

Abstract

The vascular pathogen Verticillium dahliae infects the roots of plants to cause Verticillium wilt. The molecular mechanisms underlying V. dahliae virulence and host resistance remain elusive. Here, we demonstrate that a secretory protein, VdSCP41, functions as an intracellular effector that promotes V. dahliae virulence. The Arabidopsis master immune regulators CBP60g and SARD1 and cotton GhCBP60b are targeted by VdSCP41. VdSCP41 binds the C-terminal portion of CBP60g to inhibit its transcription factor activity. Further analyses reveal a transcription activation domain within CBP60g that is required for VdSCP41 targeting. Mutations in both CBP60g and SARD1 compromise Arabidopsis resistance against V. dahliae and partially impair VdSCP41-mediated virulence. Moreover, Virus-induced silencing of GhCBP60b compromises cotton resistance to V. dahliae. This work uncovers a virulence strategy in which the V. dahliae secretory protein, VdSCP41, directly targets plant transcription factors to inhibit immunity, and reveals CBP60g, SARD1 and GhCBP60b as crucial components governing V. dahliae resistance.

Article and author information

Author details

  1. Jun Qin

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-1306-3433
  2. Kailun Wang

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Lifan Sun

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Haiying Xing

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Sheng Wang

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  6. Lin Li

    National Institute of Biological Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  7. She Chen

    National Institute of Biological Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  8. Hui-Shan Guo

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    For correspondence
    guohs@im.ac.cn
    Competing interests
    The authors declare that no competing interests exist.
  9. Jie Zhang

    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    For correspondence
    zhangjie@im.ac.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-2781-8956

Funding

The Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11020600)

  • Hui-Shan Guo
  • Jie Zhang

National Natural Science Foundation of China (31730078)

  • Jun Qin
  • Hui-Shan Guo
  • Jie Zhang

The Youth Innovation Promotion Association of Chinese Academy of Sciences

  • Jie Zhang

The Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11040500)

  • Hui-Shan Guo
  • Jie Zhang

National Natural Science Foundation of China (31571968)

  • Jun Qin
  • Hui-Shan Guo
  • Jie Zhang

National Natural Science Foundation of China (31501593)

  • Jun Qin
  • Hui-Shan Guo
  • Jie Zhang

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Gary Stacey, Reviewing Editor, University of Missouri, United States

Publication history

  1. Received: January 11, 2018
  2. Accepted: May 11, 2018
  3. Accepted Manuscript published: May 14, 2018 (version 1)

Copyright

© 2018, Qin et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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