Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates
- University of California, Davis, United States
- University of California, San Francisco, United States
Abstract
As microtubule-organizing centers of animal cells, centrosomes guide the formation of the bipolar spindle that segregates chromosomes during mitosis. At mitosis onset, centrosomes maximize microtubule-organizing activity by rapidly expanding the pericentriolar material (PCM). This process is in part driven by the large PCM protein pericentrin (PCNT), as its level increases at the PCM and helps recruit additional PCM components. However, the mechanism underlying the timely centrosomal enrichment of PCNT remains unclear. Here we show that PCNT is delivered co-translationally to centrosomes during early mitosis by cytoplasmic dynein, as evidenced by centrosomal enrichment of PCNT mRNA, its translation near centrosomes, and requirement of intact polysomes for PCNT mRNA localization. Additionally, the microtubule minus-end regulator, ASPM, is also targeted co-translationally to mitotic spindle poles. Together, these findings suggest that co-translational targeting of cytoplasmic proteins to specific subcellular destinations may be a generalized protein targeting mechanism.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for Figure 2B, Figure 3C, Figure 3- figure supplement 2, Figure 4A, Figure 4D, Figure 4- figure supplement 2, Figure 4- figure supplement 3B, Figures 5A-5C, Figure 6, and Figure 6- figure supplement 1.
Article and author information
Author details
Funding
University of California, Davis (New Faculty Startup Funds)
- Li-En Jao
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (#20169) of the University of California, Davis.
Copyright
© 2018, Sepulveda et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 6,905
- views
-
- 749
- downloads
-
- 102
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 102
- citations for umbrella DOI https://doi.org/10.7554/eLife.34959
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates
eLife 7:e34959.
https://doi.org/10.7554/eLife.34959
