(A) Synthetic mRNA encoding nuclear localized dTomato was injected into stage V-VI Xenopus laevis oocytes 1 day prior to isolation. Following isolation nuclei were incubated in OR2 buffer for the indicated times then assayed. (B) Stage VI oocyte with incision site (arrowhead) and manually isolated nucleus (dashed circle). (C) 1 hr time-lapse images of aqueously isolated and Thioflavin T (Thio T, green) stained nuclei from dTomato-NLS (red) expressing stage VI oocytes demonstrates loss of soluble dTomato, but retention of Thioflavin T positive aggregates. (D–F) Nuclei were isolated from un-manipulated oocytes, incubated in OR2, collected at 15 min intervals, and analyzed by SDS-PAGE (D). Coomassie staining (E) with quantitation (F) of soluble protein depleted nuclei demonstrates rapid loss of soluble endogenous proteins and retention of aggregate associated proteins. The number of nuclear equivalents per lane is indicated at the bottom of (E). Arrows highlight the subset of proteins enriched following depletion of soluble proteins. Arrowhead highlights 42 kDa actin, which is enriched following soluble protein depletion (F). (G) Time-lapse images of an isolated stage VI oocyte nucleus immediately following nuclear injection of Lucifer Yellow, a fluorescent ATP surrogate. Images in (C) and (G) are representative from two independent experiments encompassing at least six nuclei. Data in (F) contains three biological replicates (3–10 nuclei per replicate) representing material from two separate frogs.