(A–Q) tkvC97R heterozygous clones in the developing eye (A–I), and haltere discs (J–Q). For these experiments, hs-flp; tkvGFP>>mCherry/+ (control, (A, B, J, K), and hs-flp; tkvGFP>>C97R-mCherry/ + (experimental, (D, E, M, N) animals were heat shocked at 72 hr AEL for 10 min and discs were stained with anti-p-Mad antibody. The boxed areas in (D and M) are shown at higher magnification in (G) and (H), and (P) and (Q), respectively. While p-Mad levels in both discs were mildly disrupted, animals carrying tkvC97R heterozygous clones developed normal eyes and halteres. Scale bars: 50 μm (A, J), 0.2 mm (C), 10 μm (G, P), 100 μm (I, L).