(A) Representative images of DAPI stained oocyte diakinetic chromosomes showing abnormal chromosome morphology when drh-3, csr-1, ekl-1 and cde-1 were knocked down by RNAi in wild-type and nhl-2(ok818) animals. Scale bar 5 µm, n = 90 oocytes. (B, C, D and E) Chromosomes from each phenotype were binned in one of four categories: normal, oocytes with >6 chromosomal bodies, aggregated chromosomes, and enhanced aggregation (where aggregation exceeded that observed in wild-type animals, ***=P values <0.001). (F) Pachytene region of dissected adult male germlines stained with DAPI (red) and H3K9me2 (green) in wild-type and single and double mutants nhl-2(ok818), csr-1(tm892) and drh-3(ne4253). Wild-type males had one strong signal of H3K9me2 (yellow arrowhead), while single and double mutants had increased levels of nuclei with multiple foci (green arrowhead) or dispersed staining patterns (yellow circles) of H2K9me2 staining. Scale bar 50 µm. Over 109 nuclei were score from a minimum of 11 germline. (G) Quantification of H3K9me2 distribution in germ cells from each phenotype scored as normal, elevated, or dispersed from each phenotype. nhl-2(ok818) mutants display similar proportions of germ cells with abnormal H3K9me2 markings as the double mutants.