(A) Mitochondrial network organization in WT cells expressing Ilv3-RFP as a function of time in YPD (OD600nm: circles). Cells with a tubular (violet), a fragmented (blue), a vesicular (green), a …
Cell category scoring for each replicated experiment in Figure 1 panel A, B and F to H.
(A) Proliferation in YPD or YPGE of WT cells of the indicated genotype monitored by measuring OD600nm as a function of time. (B) Mitochondrial network organization of WT cells expressing Ilv3-RFP …
Cell category scoring for each replicated experiment in Figure 1—figure supplement 1 panel A, B and F.
(A) WT cells expressing Ilv3-RFP were grown 7 d in YPD and stained with methylene blue to reveal dead cells. Cells surrounded by a white dashed line are methylene blue positive. The histogram shows …
Cell category scoring for each replicated experiment in Figure 2 panel A, B, C, D and E to G.
(A) Upon quiescence exit, the mitochondrial network re-forms tubule before bud emergence. WT cells were grown for 7 days in YPD and allow to exit quiescence in new YPD liquid medium. The morphology …
Cell category scoring for each replicated experiment in Figure 2—figure supplement 1 panel A, C, E, F and G.
(A) Cell volume distribution of WT cells grown for 7, 14, and 21 d in YPD. In A to C, red dashed lines define three sub-populations each representing 1/3rd of the total population. Dead cells (black …
Cell category scoring for each replicated experiment in Figure 3 panel A, B, and C.
(A) WT prototroph cells grown for the indicated time in YPD were individually separated by micro-manipulation onto YPD plates according to their volume (n = 120, N = 2). Representative images of the …
Cell category scoring for each replicated experiment in Figure 3—figure supplement 1 panel A and B.
(A–D) WT cells expressing Ilv3-RFP were grown 7 d in YPD, stained with calcofluor white and refed onto a YPD microscope pad. (A) Percentage of mother or daughter cells with vesicular mitochondria …
Cell category scoring for each replicated experiment in Figure 4 panel A, B to D, E and F.
WT cells expressing Ilv3-RFP and Abp1-3xGFP (actin) or Scl1-GFP (proteasome) were grown 7 d in YPD and refed on a YPD microscope pad. (A) Histograms show the percentage of individual cells …
Cell category scoring for each replicated experiment in Figure 5 panel A, B and D.
(A) Actin phalloidin staining of WT prototroph rho zero cells after 24 hr of culture in YPD. The image is a superposition of the DIC and the RFP channel. (B) Proteasome (Scl1-GFP) localization in WT …
Cell volume measurements in daughter and mother cells depending on their mitochondrial network organization (panel C).
Resource | Designation | Source or reference | Identifiers |
---|---|---|---|
Software | ImageJ | Department of Health and Human Services, NIDA | RRID:SCR_003070 |
Software | GraphPad Prism 5 | GraphPad Software, Inc. La Jolla, USA | RRID:SCR_015807 |
Table with the genotype of the strains used in this study.