(A) Average tie-courses of mean fEPSPs after 10 Hz LTD protocol (1à min, arrow) in WIN-exposed mPFC slices preincubated for 45 min in 2 μM URB597, a FAAH inhibitor. (B) Individual experiments (grey) and group averages (tan circles), before (baseline) and after (40 min) LTD induction showing that, in WIN-exposed male (WIN, n = 17, green circle), LTD is restored following bath-application of the FAAH inhibitor URB597 (2 μM, 45 min preincubation, URB, n = 6; pink circle): 0.207 ± 0.017 mV before and 0.165 ± 0.007 after LTD induction (n = 6; p<0.05, paired t-test). (C) The URB597 LTD rescue requires CB1R. Time course of fEPSP during 10 Hz stimulus evoked LTD (arrow, 10 min) in presence of CB1R antagonist SR141716 (SR, 4 μM, n = 8, blue circles) or TRPV1 antagonist Capsazepine (CPZ, 10 μM, n = 3, black circles) in WIN-prenatally exposed males. (D) Individual experiments: SR141716A preincubation prevented from the URB597 restoration of LTD: 0.222 ± 0.028 mV before and 0.217 ± 0.026 after LTD induction (blue circle, n = 8; p>0.05, paired t-test). In contrast, the TRPV1 antagonist did not prevent from URB597 rescue: 0.214 ± 0.042 mV before LTD and 0.170 ± 0.030 mV after LTD induction (black circle, n = 3; p<0.05, paired t-test). (E–F) Systemic administration of URB597 (0.1 mg/kg, i.p.) normalized the altered social behavior in male rats prenatally exposed to WIN (numbers of contacts: E: F(WIN in utero x URB)1,15=9.341. p<0.05, two-way ANOVA; time interaction: F: F(WIN in utero x URB)1,15=3.421, p=0.08, two-way ANOVA). (G-H) Pre-treatment with the CB1R antagonist SR141716A (1 mg/kg, i.p.) prevented the curating actions of URB597 (0.1 mg/kg, i.p.) on social interaction. In contrast, the TRPV1 antagonist capsazepine did not block the curating effects of URB597 on social interaction in adult rats prenatally exposed to WIN55,212–2 (number of contacts: G: F3,34=21.02, p<0.05, one-way ANOVA; time interaction: H: F3,34=18.34, p<0.05, one-way ANOVA). Scatter dot plot represents each animal. Error bars indicate SEM. *p<0.05 Student–Newman–Keuls test.