Irreversible temperature gating in trpv1 sheds light on channel activation
- Universidad Nacional Autónoma de México, Mexico
Abstract
Temperature activated TRP channels or thermoTRPs are among the only proteins that can directly convert temperature changes into changes in channel open probability. In spite of a wealth of functional and structural information, the mechanism of temperature activation remains unknown. We have carefully characterized the repeated activation of TRPV1 by thermal stimuli and discovered a previously unknown inactivation process, which is irreversible. We propose that this form of gating in TRPV1 channels is a consequence of the heat absorption process that leads to channel opening.
Data availability
Summary data for Figures 1, 2, 3, and 4 have been provided as source data files. The electrophysiological recordings will be made available upon request to the corresponding author.
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Author details
Funding
Consejo Nacional de Ciencia y Tecnología (CB-2015-252644)
- Leon D Islas
DGAPA-PAPIIT-UNAM (IN209515)
- Leon D Islas
DGAPA-PAPIITT-UNAM (IN200717)
- Tamara Rosenbaum
Consejo Nacional de Ciencia y Tecnología (CB-2014-01-238399)
- Tamara Rosenbaum
Consejo Nacional de Ciencia y Tecnología (Fronteras de la Ciencia 77)
- Tamara Rosenbaum
- Leon D Islas
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Sánchez-Moreno et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Irreversible temperature gating in trpv1 sheds light on channel activation
eLife 7:e36372.
https://doi.org/10.7554/eLife.36372
