(A) P. pyralis males emitting their characteristic swooping ‘J’ patrol flashes over a field in Homer Lake, Illinois. Females cue in on these species-specific flash patterns and respond with their …
(A) Assembled Ppyr1.3 linkage groups with annotation of the location of known luminescence-related genes, combined with Hi-C linkage density maps. Linkage group 3a (box with black arrow) corresponds …
Figure 2E. Orthogroup clustering analysis.
Excel file of Figure 2F table.
(A) The reaction scheme of firefly luciferase is related to that of fatty acyl-CoA synthetases. (B) Model for genomic evolution of firefly luciferases. Ranging from genome structures of luciferase …
Gene tree, gene accession numbers, annotation, and expression values for Figure 3C.
Bash scripts for Figure 3D figure production.
(A) Ancestral state reconstruction recovers at least two gains of luciferase activity in bioluminescent beetles. Luciferase activity (top right figure key; black: luciferase activity, white: no …
NEXUS and Newick files for luciferase ancestral state reconstruction in Figure 4A.
Gene tree, metadata, and coding nucleotide multiple sequence alignment for Elaterid luciferase homolog branch selection test.
Enzymes which are highly expressed (HE), differentially expressed (DE), and annotated as enzymes via InterProScan are shown in the Venn diagrams for their respective species. Those genes in the …
Table of Figure 5 highly expressed, differentially expressed, orthogroup overlapped genes.
(A) Hypothesized lucibufagin biosynthetic pathway, starting from cholesterol. (B) LC-HRAM-MS multi-ion-chromatograms (MIC) showing the summation of exact mass traces for the [M + H]+ of 11 …
CYP303 multiple sequence alignment and gene tree for Figure 6C.
P. pyralis sightings (red circles show county centroided reports) in the United States and Ontario, Canada (diagonal hashes). The World Wildlife Fund Terrestrial Ecoregions (Olson et al., 2001; World…
(A) Ventral and (B) side view of a P. pyralis aedeagus dissected from specimens collected on the same date and locality as those used for PacBio sequencing. Note the strongly sclerotized paired …
(A) Photograph under ambient light of ~1 day post-deposition P. pyralis eggs. (B) Photograph of self-luminescence of ~1 day post-deposition P. pyralis eggs. Both photographs taken with a NightOwl …
Both P. pyralis larvae (red arrows), and Enchytraeus albidus (yellow arrows), were observed to feed on the paralyzed earthworms.
(A) Linear and (B) log plot of a kmer spectral genome composition analysis of the ‘8369’ P. pyralis Illumina short-read library from a single P. pyralis XO adult male (Appendix 1.5.1; Appendix …
Lane 1 was used for further library prep and sequencing, Lanes 2–5 represent separate batches of P. pyralis HMW DNA that was not used for PacBio sequencing. Lane 1 was used as it had the highest DNA …
Figure produced with SMRTPortal (v2.3.0.140936, Pacific Biosciences, 2017) by aligning all PacBio reads from data from the 61 SMRT cells against Ppyr1.3 using the RS_Resequencing.1 protocol with …
Coverage shown represents mean coverage of reads from the Illumina short-insert library (Sample name 8369; Appendix 4—table 1), aligned against Ppyr1.2 using Bowtie2 with parameters (--local). …
Coverage shows represents mean coverage of reads from the PacBio library (Sample name 1611; Appendix 4—table 1). The reads were aligned using SMRTPortal v2.3.0.140893 with the ‘RS_Resequencing.1’ …
(A) The blue set represents scaffolds which have >10.0 coverage in both Illumina and PacBio libraries. (B) The red set represents scaffolds which had either genes on repeats (non simple or …
The mitochondrial genome of P. pyralis was assembled and annotated as described. Note the firefly specific tandem-repeat-unit (TRU) region. Figure produced with Circos (Krzywinski et al., 2009).
Neighbor-joining phylogenetic tree of 165 cytochrome P450s from P. pyralis. Four pseudogenes and one short sequence were removed. The P450 clans have colored spokes (CYP2 clan brown, CYP3 clan …
(A) Linear and (B) log plot of a kmer spectral genome composition analysis of the ‘FFGPE_PE200’ A. lateralis Illumina short-insert library (Appendix 2.5; Appendix 4—table 1) with jellyfish (v2.2.9; …
Coverage shown represents mean coverage of reads from the Illumina short-insert library (Sample name FFGPE_PE200; Appendix 4—table 1), aligned against Alat1.2 using Bowtie2. Scaffolds were …
(A) Dorsal and (B) ventral view of an Ignelater luminosus aedeagus, dissected from the same batch of specimens used for linked-read sequencing and genome assembly. The species identity of this …
(A) Linear and (B) log plot of a kmer spectral genome composition analysis of the ‘1610_IlumiHiSeqX’ I. luminosus Illumina linked-read library (Appendix 2.5; Appendix 4—table 1) with jellyfish …
Coverage shown represents mean coverage of reads from the HiSeqX Chromium library sequencing (Sample name 1610_IlumiHiSeqX; Appendix 4—table 1), aligned against Ilumi1.0 using Bowtie2 with …
Alignment performed in in Gepard (Krumsiek et al., 2007). Note the large (10 kbp+) tandem repetitive region.
Diagram of the manual merge of Ilumi1.1_Scaffold13255 with Ilumi1.1_Scaffold11560 between I. luminosus genome assembly versions Ilumi1.1 and Ilumi1.2. This merge was supported by: (1) The putative …
The mitochondrial genome of I. luminosus was assembled and annotated as described. in the Appendix 3.10. Figure produced with Circos (Krzywinski et al., 2009).
Orthogroups were calculated between the PPYR_OGS1.1, AQULA_OGS1.0, ILUMI_OGS1.2, genesets, and the T. casteneum and D. melanogaster filtered Uniprot reference proteomes using OrthoFinder(Emms and …
Levels and patterns of mCG in P. pyralis are corroborated by the presence of de novo and maintenance DNMTs (DNMT3 and DNMT1, respectively). Notably, P. pyralis possesses two copies of DNMT1, and 3 …
(A) Intron-exon structure of P. pyralis and A. lateralis Luc1 and Luc2 from Ppyr1.3 and Alat1.3, and IlumLuc from Ilumi1.2. Between fireflies and click-beetles, the structure of the luciferase genes …
MAFFT (Katoh and Standley, 2013) L-INS-i multiple sequence alignment of luciferase gene nucleotide sequences from PpyrOGS1.1 and AlatOGS1.0 demonstrates the location of intron-exon junctions (bolded …
A preliminary maximum likelihood tree was reconstructed from a 385 amino acid multiple sequence alignment, generated via a BLASTP and orthoDB search using P. pyralis luciferase as query (e-value: …
Amino acid variation of extant Elaterid luciferases (Clade D ‘Eluc’ subset; Figure 3) at all sites recovered via both the MEME and PAML-BEB selection analysis (Appendix 4—table 5). Site numbering …
Peptide sequences from P. pyralis, A. lateralis, I. luminosus, T. castaneum, and D. melanogaster were clustered (orthogroup # 698), multiple sequence aligned, and refactored into a species rooted …
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
Figure produced using MZmine2 (Pluskal et al., 2010).
(A) MS2 similarity network produced with the MZmine2 MS2 similarity search module. Nodes represent MS2 spectra from the initial dataset, whereas edges represent an MS2 similarity match between two …
The mitochondrial genome of A. antennatus was assembled and annotated as described in the Appendix 5.2, and taxonomically identified as described in Appendix 5.3. Figure produced with Circos (Krzywin…
(A) Phylogenetic tree based in MAFFT alignments of predicted replicases of Orthomyxoviridae (OMV) ICTV accepted viruses (green stars), new Photinus pyralis viruses (underlined) and tentative …
N: number of individuals pooled for sequencing; Sex/stage: M = male, F = female, A = adult, L = larva, L1 = larva 1 st instar, L4 = larvae fourth instar, E13 = 13 days post fertilization eggs; Tissue…
Library name | Source* | SRA ID | N | Sex/stage | Tissue | Library type |
---|---|---|---|---|---|---|
8175 Photinus pyralis male head (adult) transcriptome | SRA1 | SRR2103848 | 1 | M/A | H | |
8176 Photinus pyralis male light organ (adult) transcriptome | SRA1 | SRR2103849 | 1 | M/A | PA | |
8819 Photinus pyralis light organ (larval) transcriptome | SRA1 | SRR2103867 | 1 | L | PA | |
9_Photinus_sp_1_lantern | SRA2 | SRR3521424 | 1 | M/A | PA | Strand-specific. Ribo-zero |
Ppyr_FatBody_1 | SRA3 | SRR3883756 | 6 | M/A | FB | |
Ppyr_FatBody_2 | SRA3 | SRR3883757 | 6 | M/A | FB | |
Ppyr_FatBody_3 | SRA3 | SRR3883766 | 6 | M/A | FB | |
Ppyr_FatBody_Mated | SRA3 | SRR3883767 | 4 | M/A | FB | |
Ppyr_FThorax | SRA3 | SRR3883768 | 3 | F/A | T | |
Ppyr_MThorax_1 | SRA3 | SRR3883769 | 6 | M/A | T | |
Ppyr_MThorax_2 | SRA3 | SRR3883770 | 6 | M/A | T | |
Ppyr_MThorax_3 | SRA3 | SRR3883771 | 6 | M/A | T | |
Ppyr_OAG_1A | SRA3 | SRR3883772 | 6 | M/A | AG | |
Ppyr_OAG_1B | SRA3 | SRR3883773 | 6 | M/A | AG | |
Ppyr_OAG_2 | SRA3 | SRR3883758 | 6 | M/A | AG | |
Ppyr_OAG_Mated | SRA3 | SRR3883759 | 4 | M/A | AG | |
Ppyr_SDGBursa | SRA3 | SRR3883760 | 3 | F/A | SD | |
Ppyr_SG_Mated | SRA3 | SRR3883761 | 4 | M/A | SG | |
Ppyr_Spermatheca | SRA3 | SRR3883762 | 3 | F/A | SC | |
Ppyr_SpiralGland_1 | SRA3 | SRR3883763 | 6 | M/A | SG | |
Ppyr_SpiralGland_2 | SRA3 | SRR3883764 | 6 | M/A | SG | |
Ppyr_SpiralGland_3 | SRA3 | SRR3883765 | 6 | M/A | SG | |
Ppyr_Lantern_1A | ‡ | SRR6345453 | 6 | M/A | P | |
Ppyr_Lantern_2 | ‡ | SRR6345454 | 6 | M/A | P | |
Ppyr_Lantern_3 | ‡ | SRR6345446 | 6 | M/A | P | |
Ppyr_Eggs | ‡ | SRR6345447 | 7 | E13 | E | Strand-specific |
Ppyr_Larvae | ‡ | SRR6345445 | 4 | L1 | WB | Strand-specific |
Ppyr_wholeFemale† | ‡ | SRR6345449 | 1 | F/A | WB | Strand-specific |
Ppyr_wholeMale | ‡ | SRR6345452 | 1 | M/A | WB | Strand-specific |
TF_VA2017_3pooled_larval_lantern | ‡ | SRR7345580 | 3 | L4 | P |
*SRA1 = NCBI BioProject PRJNA289908 (Sander and Hall, 2015); SRA2 = NCBI BioProject PRJNA321737 (Fallon et al., 2016); SRA3 = NCBI BioProject PRJNA328865 (Al-Wathiqui et al., 2016).
†Parent of eggs and larvae with data from this study.
‡This study.
Repeat class | Family | Counts | Bases | % of assembly |
---|---|---|---|---|
DNA | All | 122551 | 38364685 | 8.14 |
Helitrons | 35068 | 9308100 | 1.97 | |
LTR | All | 28860 | 11401648 | 2.42 |
Non-LTR | All | 52107 | 17744320 | 3.76 |
LINE | 48983 | 16763499 | 3.56 | |
SINE | 1241 | 139637 | 0.03 | |
Unknown interspersed | 696511 | 141970977 | 30.1 | |
Complex tandem repeats | 10395 | 2352796 | 0.50 | |
Simple repeat | 48224 | 2372183 | 0.50 | |
rRNA | 449 | 161517 | 0.034 |
Liliana Bachrach | Doug Fambrough | Benjamin Lower | Luis Cunha | Joshua Guerriero |
---|---|---|---|---|
Atsuko Fish | Tom Alar | Noreen Huefner | David Esopi | John Skarha |
Rutong Xie | Richard Hall | Zachary Michel | Jack Hynes | Keith Guerin |
Nathan Shaner | Joe Doggett | Joe T. Bamberg | Michael McGurk | Pureum Kim |
Sara Lewis | Mark Lewis | Lauren Solomon | Peter Berx | Milo Grika |
Jing-Ke Weng | Sarah Sander | Dr. Husni Elbahesh | Matt Grommes | Daniel Zinshteyn |
Peter Rodenbeck | Daniel Bear | Kathryn Larracuente | Colette Dedyn | Tom Brekke |
Larry Fish | Don Salvatore | Matthew Cichocki | Florencia Schlamp | Edoardo Gianni |
Amanda Larracuente | Emily Davenport | Marcel Bruchez | Marie Lower | Cindy Wu |
Hunter Lower | Ted Sharpe | Robert Unckless | Michael R. McKain | Christina Tran |
Allan Kleinman | David Plunkett | Arvid Ågren | Ben Pfeiffer | Eric Damon Walters |
Misha Koksharov | Tim Fallon | Margaret S Butler | Kathryn Keho | Geoffrey Giller |
Sarah Shekher | Edward Garrity | Yasir Ahmed-Braimah | Jenny Wayfarer | Fahd Butt |
Jared Lee | Huaping Mo | Ruth Ann Grissom | Darby Thomas | Christophe Mandy |
Raphael De Cock | TimG | Tomáš Pluskal | Emily Hatas | |
Linds Fallon | Jan Thys | Genome Galaxy | Richard Casey | |
Grace Li | Francisco Martinez Gasco | Dustin Greiner | William Nicholls |
N: number of individuals pooled for sequencing; Sex/stage: M = male, F = female, A = adult, L = larva, L = larvae, E = Eggs, p=Pupae, P-E = Pupae early, P-M = Pupae middle, P-L = Pupae late; Tissue: …
Library name | Label | SRA ID | N | Sex/ Stage | Tissue | Library type |
---|---|---|---|---|---|---|
R102L6_idx13 | BdM1 | DRR119264 | 1 | M/A | B | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx25 | BdM2 | DRR119265 | 1 | M/A | B | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx27 | BdM3 | DRR119266 | 1 | M/A | B | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx15 | HeF1 | DRR119267 | 3 | F/A | H | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx22 | HeF2 | DRR119268 | 3 | F/A | H | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx23 | HeF3 | DRR119269 | 3 | F/A | H | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx12 | HeM1 | DRR119270 | 2 | M/A | H | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx20 | HeM2 | DRR119271 | 2 | M/A | H | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx21 | HeM3 | DRR119272 | 2 | M/A | H | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx16 | LtF1 | DRR119273 | 5 | F/A | La | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx06 | LtF2 | DRR119274 | 5 | F/A | La | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx12 | LtF3 | DRR119275 | 5 | F/A | La | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx14 | LtM1 | DRR119276 | 5 | M/A | La | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx05 | LtM2 | DRR119277 | 5 | M/A | La | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx19 | LtM3 | DRR119278 | 5 | M/A | La | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx15 | WAF1 | DRR119279 | 1 | F/A | W | Illumina single-end, non-stranded specific, PolyA |
R128L1_idx16 | WAF2 | DRR119280 | 1 | F/A | W | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx18 | WAF3 | DRR119281 | 1 | F/A | W | Illumina single-end, non-stranded specific, PolyA |
R128L1_idx11 | WAM1 | DRR119282 | 1 | M/A | W | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx13 | WAM2 | DRR119283 | 1 | M/A | W | Illumina single-end, non-stranded specific, PolyA |
R128L1_idx14 | WAM3 | DRR119284 | 1 | M/A | W | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx4 | Egg1 | DRR119285 | 19.6 mg (~30–50) | E ~6 hr AEL | W | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx01 | Egg2 | DRR119286 | 21.6 mg (~30–50) | E ~7 d AEL | W | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx5 | Lrv1 | DRR119287 | 1 | L | W | Illumina paired-end, non-stranded specific, PolyA |
R128L1_idx03 | Lrv2 | DRR119288 | 1 | L | W | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx04 | Lrv3 | DRR119289 | 1 | L | W | Illumina single-end, non-stranded specific, PolyA |
R128L1_idx07 | PpEM | DRR119290 | 1 | M/P-E | W | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx10 | PpLF | DRR119291 | 1 | F/P-L | W | Illumina single-end, non-stranded specific, PolyA |
R128L1_idx09 | PpMF | DRR119292 | 1 | F/P-M | W | Illumina single-end, non-stranded specific, PolyA |
R128L2_idx08 | PpMM | DRR119293 | 1 | M/P-M | W | Illumina single-end, non-stranded specific, PolyA |
R102L6_idx7 | PpEF | DRR119294 | 1 | F/P-E | W | Illumina paired-end, non-stranded specific, PolyA |
R102L6_idx6 | PpLM | DRR119295 | 1 | M/P-L | W | Illumina paired-end, non-stranded specific, PolyA |
Repeat class | Family | Counts | Bases | % of assembly |
---|---|---|---|---|
DNA | All | 229064 | 73263593 | 8.06 |
Helitrons | 930 | 466679 | 0.051 | |
LTR | All | 59499 | 23391956 | 2.57 |
Non-LTR | All | 151788 | 50394853 | 5.55 |
LINE | 151788 | 50394853 | 5.55 | |
SINE | 0 | 0 | 0 | |
Unknown interspersed | 450934 | 99998958 | 11.01 | |
Complex tandem repeats | 295 | 33237 | 0.004 | |
Simple repeat | 155265 | 6656757 | 0.73 | |
rRNA | 0 | 0 | 0 |
Repeat name | Repeat unit length | Repeat unit sequence |
---|---|---|
Ilumi.complex.repeat.1 | ~100 bp | TGGTACGAACTATACACGTATACTCAAATCTAATT GTGATACAGCAAAGTAATAATGCAGCATTGTTTGCC GCTCTATACTGCGATTTTATAGTGGT |
Library name | SRA ID | N | Sex | Tissue | Notes |
---|---|---|---|---|---|
Pyrophorus_luminosus_head | SRR6339835 | 1 | M* | Prothorax and head (lantern containing) | Illumina RNA-Seq |
Prothorax_A3 | SRR6339834 | 1 | M* | Prothorax and head (lantern containing) | BGISEQ-500 RNA-Seq |
Thorax_A3 | SRR6339833 | 1 | M* | Mesothorax and metathorax | BGISEQ-500 RNA-Seq |
Abdomen_A3 | SRR6339832 | 1 | M* | Abdomen (lantern containing) | BGISEQ-500 RNA-Seq |
Prothorax_A4 | SRR6339831 | 1 | M* | Prothorax and head (lantern containing) | BGISEQ-500 RNA-Seq |
Thorax_A4 | SRR6339830 | 1 | M* | Mesothorax and metathorax | BGISEQ-500 RNA-Seq |
Abdomen_A4 | SRR6339838 | 1 | M* | Abdomen (lantern containing) | BGISEQ-500 RNA-Seq |
*Gender inferred. See Appendix 3.3 for a discussion on this inference.
Repeat class | Family | Counts | Bases | % of assembly |
---|---|---|---|---|
DNA | All | 158853 | 71221843 | 8.45 |
Helitrons | 344 | 139863 | 0.016 | |
LTR | All | 23433 | 11341577 | 1.35 |
Non-LTR | All | 151788 | 50394853 | 4.75 |
LINE | 97703 | 40052840 | 4.75 | |
SINE | 0 | 0 | 0 | |
Unknown interspersed | 757206 | 159587269 | 18.93 | |
Complex tandem repeats | 4976 | 848992 | 0.1 | |
Simple repeat | 108914 | 4439967 | 0.52 | |
rRNA | 0 | 0 | 0 |
ID: NCBI BioProject or Gene Expression Omnibus (GEO) ID. N: Number of individuals used for sequencing. Date: collection date for wild-caught individuals. Locality: GSMNP: Great Smoky Mountains …
Library | SRA ID | N | Date | Locality | Sex | Tissue | Type | Reads | Number | Cov | Insert size (Ori) | Contamination |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Photinus pyralis | ||||||||||||
8369* | SRR6345451/ SRR2127932 | 1 | 6/13/11 | GSMNP | M | Thr | SI | 101 × 101 PE | 203,074,230 | 98 (49) | 354 bp (FR) | 0.28 |
8375_3 K† | SRR6345448 | 1 | 6/13/11 | GSMNP | M | Thr | MP | 101 × 101 PE | 101,624,630 | 21 | 2155 bp (RF) | 2.63 |
8375_6 K† | SRR6345457 | 1 | 6/13/11 | GSMNP | M | Thr | MP | 101 × 101 PE | 23,564,456 | 5 | 4889 bp (RF) | 3.36 |
83_3 K† | SRR6345450 | 3 | 6/13/11 | GSMNP | M | Thr | MP | 101 × 101 PE | 121,757,858 | 13 | 2247 bp (RF) | 0.79 |
83_6 K† | SRR6345455 | 3 | 6/13/11 | GSMNP | M | Thr | MP | 101 × 101 PE | 17,905,700 | 1 | 4877 bp (RF) | 1.38 |
1611_PpyrPB1 | SRX3444870 | 4 | 7/9/16 | MMNJ | M | WB | PB | CLR-PB | 3,558,201 | 38 (21) | 7 Kbp‡ | 3.5 |
1704 | SRR6345456 | 2 | 7/9/16 | MMNJ | M | WB | HC | 80 × 80 PE | 93,850,923 | ND | ND | ND |
1705 | GSE107177 | 1 | 7/9/16 | MMNJ | M | WB | BS | 150 SE | 113,761,746 | ~16x§ | ND | ND |
Aquatica lateralis | ||||||||||||
FFGPE_PE200 | DRR119296 | 1 | N/A | IY90 | F | WB | SI | 126 × 126 PE | 561,450,686 | 72 | 180 bp (FR) | ND |
FFGPE_PE800 | DRR119297 | WB | SI | 126 × 126 PE | 218,830,950 | 20 | 476 bp (FR) | ND | ||||
FFGMP_MPGF | DRR119298 | WB | MP | 101 × 101 PE | 358,601,808 | 31 | 2300 bp (RF) | ND | ||||
Ignelater luminosus | ||||||||||||
1610_Ilumi HiSeqX# | SRR6339837 | 1 | MAPR | M¶ | WB | CH | 151 × 151 PE | 408,838,927 | 99 | 339 bp (FR) | ND | |
1706_Ilumi HiSeq2500# | SRR6339836 | WB | CH | 150 × 150 PE | 145,250,480 | 48 | 334 bp (FR) | ND | ||||
18_lib1 | SRR6760567 | ONT | CLR | 824,248 | ~2x | 2984‡ |
*Mean of three sequencing lanes
†Mean of two sequencing lanes
‡Mean subread (PacBio) or read (Oxford Nanopore) length after alignment
§Estimate from quantity of mapped reads
#Same library, different instruments
¶Inferred from specimens collected at the same time and locality
Assembly | Libraries | Assembly scheme | Assembly* /measured** genome size (Gbp) | Scaffold/ Contig (#) | Contig NG50*** (Kbp) | Scaffold NG50*** (Kbp) | BUSCO statistics |
---|---|---|---|---|---|---|---|
Ppyr0.1-PB | PacBio (61 RSII SMRT cells) | Canu (no polishing) | 721/422 | 25986/ 25986 | 86 | 86 | C:93.8%[S:65.2%,D:28.6%],F:3.3%,M:2.9% |
Ppyr1.1 | Short read Mate Pair PacBio | MaSuRCA + redundancy reduction | 473/422 | 8065/ 8285 | 193.4 | 202 | C:97.2% [S:88.8%, D:8.4%], F:1.9%, M:0.9% |
Ppyr1.2 | Short | Ppyr1.1+ Phase Genomics scaffolder (in-house) | 473/422 | 2535/ 7823 | 193.4 | 50,607 | C:97.2% [S:88.8% ,D:8.4%],F:1.9%, M:0.9% |
PacBio | |||||||
Hi-C | |||||||
Ppyr1.3 | Short read Mate Pair PacBio | Ppyr1.2 +Blobtools + manual filtering | 472/422 | 2160/ 7533 | 192.5 | 49,173 | C:97.2% [S:88.8%, D:8.4%], F:1.9%, M:0.9% |
Alat1.2 | Short read Mate Pair | ALLPATHS-LG | 920/940 | 7313/ 36467 | 38 | 673 | C:97.4% [S:96.2%, D:1.2%], F:1.8%, M:0.8% |
Alat1.3 | Short read Mate Pair | Alat1.2+Blobtools + manual filtering | 909/940 | 5388/ 34298 | 38 | 670 | C:97.4% [S:96.2%, D:1.2%], F:1.8%, M:0.8% |
Ilumi1.0 | Linked-read | Supernova | 845/764 | 91560/ 105589 | 31.6 | 116.5 | C:93.7% [S:92.3%, D:1.4%], F:4.3%, M:2.0%, |
Ilumi1.2 | Linked read+ nanopore | Ilumi1.0+Blobtools+ Pilon indeland gappolishing. Manual scaffolding | 842/764 | 91305/ 105262 | 34.5 | 115.8 | C:94.8% [S:93.4%, D:1.4%],F:3.5%, M:1.7% |
*Calculated from genome assembly file with ‘seqkit stat’
**Measured via flow cytometry of propidium iodide stained nuclei. See Appendix 1.4, 2.4, 3.4.
***Calculated with QUAST (v4.5) (Gurevich et al., 2013), parameters ‘-e --scaffolds --est-ref-size X --min-contig 0’ and the measured genome size for ‘est-ref-size’
Species | Genome version (NCBI assemblies) | Note | Genome BUSCO (endopterygota_odb9) | Protein geneset BUSCO (endopterygota_odb9)** |
Drosophila melanogaster | GCA_000001215.4 Release 6 | Model insect | C:99.4%[S:98.7%,D:0.7%], F:0.4%,M:0.2%,n:2442 | C:99.6%[S:92.8%,D:6.8%], F:0.3%,M:0.1%,n:2442 |
Tribolium castaneum | GCF_000002335.3 Release 5.2 | Model beetle | C:98.4%[S:97.9%,D:0.5%], F:1.2%,M:0.4%,n:2442 | C:98.0%[S:95.8%,D:2.2%], F:1.6%,M:0.4%,n:2442 |
Photinus pyralis* | Ppyr1.3* | North American firefly | C:97.2%[S:88.8%,D:8.4%], F:1.8%,M:1.0%,n:2442 | C:94.2%[S:84.0%,D:10.2%], F:1.2%,M:4.6%,n:2442 |
Aquatica lateralis* | Alat1.3* | Japanese firefly | C:97.4%[S:96.2%,D:1.2%], F:1.8%,M:0.8% | C:90.0%[S:89.1%,D:0.9%], F:3.2%,M:6.8%,n:2442 |
Nicrophorus vespilloides (Cunningham et al., 2015) | GCF_001412225.1 Release 1.0 | Burying beetle | C:96.8%[S:95.3%,D:1.5%], F:2.1%,M:1.1%,n:2442 | C:98.7%[S:69.4%,D:29.3%], F:0.8%,M:0.5%,n:2442 |
Agrilus planipennis (Poelchau et al., 2015) | GCF_000699045.1 Release 1.0 | Emerald Ash Borer beetle | C:92.7%[S:91.8%,D:0.9%], F:4.6%,M:2.7%,n:2442 | C:92.1%[S:64.1%,D:28.0%], F:4.5%,M:3.4%,n:2442 |
Ignelater luminosus* | Ilumi1.2 | Puerto Rican bioluminescent click beetle | C:94.8%[S:93.4%,D:1.4%], F:3.5%,M:1.7%,n:2442 | C:91.8%[S:89.8%,D:2.0%], F:4.4%,M:3.8%,n:2442 |
*=This report, **=Protein genesets downloaded from the NCBI Genome resource associated with the mentioned assembly in the 2nd column, or in the case of D. melanogaster, and T. castaneum, protein genesets were produced from Uniprot Reference Proteomes which had been heuristically filtered down to ‘canonical’ isoforms with a custom script and BLASTP against the D. melanogaster, T. castaneum, Apis mellifera, Bombyx mori, Caenorhabditis elegans, and Anopheles gambiae protein genesets associated with their more recent genome assembly on NCBI. See Appendix 4.2.1 for more detail.
Proportion indicates the proportion of sites in each site class (0, 1, 2a, 2b). Site classes 0 and 1 are those in the constrained and neutral classes, respectively. 2a are sites that were …
Hypothesis | Site class: | 0 | 1 | 2a | 2b | lnL |
---|---|---|---|---|---|---|
H0: no selection | proportion | 0.62 | 0.14 | 0.18 | 0.04 | −15888.16 |
background ω | 0.12 | 1 | 0.12 | 1 | ||
foreground ω | 0.12 | 1 | 1 | 1 | ||
HA: selection | proportion | 0.71 | 0.15 | 0.11 | 0.02 | −15833.50* |
background ω | 0.12 | 1 | 0.12 | 1 | ||
foreground ω | 0.12 | 1 | 3.25 | 3.25 |
*significant (LRT: 9.32, df = 1)
Site numbering | MEME2 | PAML-BEB | |||||||
---|---|---|---|---|---|---|---|---|---|
MSA | IlumLuc | IlumLuc site AA1 | α | β+ | LRT | Episodic selection p-value | # branches | BEB site class probability | BEB significance |
28 | 28 | M | 0.986 | * | |||||
34 | 34 | K | 0.47 | 23.5 | 4.1 | 0.0603 | 0 | ||
41 | 41 | Q | 0.5 | ||||||
46 | 44 | V | 0 | 3 | 4.5 | 0.0485 | 0 | ||
49 | 47 | I | 0.93 | 792.4 | 3.8 | 0.0692 | 0 | ||
50 | 48 | G | 0.57 | 3332.3 | 4.8 | 0.0427 | 0 | 0.836 | |
72 | 70 | N | 0.55 | 3333.1 | 3.1 | 0.0998 | 0 | 0.776 | |
77 | 75 | M | 0.964 | * | |||||
85 | 83 | A | 0.962 | * | |||||
89 | 87 | K | 0.958 | * | |||||
99 | 97 | W | 0.598 | ||||||
105 | 103 | V | 0.44 | 6.8 | 4.3 | 0.0549 | 0 | 0.768 | |
118 | 116 | C | 0.3 | 3333.1 | 7.4 | 0.0109 | 1 | ||
122 | 120 | G | 0.82 | ||||||
146 | 144 | L | 0.34 | 12.8 | 4.9 | 0.039 | 0 | ||
147 | 145 | G | 0.75 | 3333.6 | 5.9 | 0.0236 | 0 | ||
172 | 170 | A | 0.698 | ||||||
189 | 185 | F | 0.534 | ||||||
223 | 219 | L | 0.507 | ||||||
226 | 222 | T | 1.44 | 29.6 | 4.8 | 0.0427 | 0 | 0.889 | |
234 | 230 | I | 1.13 | 9.6 | 3.1 | 0.0991 | 0 | 0.613 | |
279 | 275 | A | 0.559 | ||||||
290 | 286 | N | 0.92 | 3333 | 4 | 0.064 | 0 | ||
315 | 311 | L | 0.69 | 29.5 | 5.1 | 0.0362 | 0 | 0.884 | |
329 | 325 | L | 0.766 | ||||||
337 | 333 | P | 0.26 | 13.3 | 6.3 | 0.0198 | 0 | ||
341 | 337 | C | 0.812 | ||||||
365 | 361 | L | 0.58 | 7.6 | 4.4 | 0.052 | 0 | 0.912 | |
369 | 365 | T | 0.21 | 6.8 | 6.6 | 0.0169 | 0 | 0.843 | |
379 | 375 | R | 0.932 | ||||||
383 | 379 | E | 0 | 2.8 | 4.1 | 0.0594 | 0 | ||
389 | 385 | Q | 0.792 | ||||||
398 | 394 | P | 0.96 | 1999.2 | 4.5 | 0.05 | 0 | 0.951 | * |
401 | 397 | S | 0.617 | ||||||
406 | 402 | N | 0.58 | 5.5 | 3.7 | 0.0745 | 0 | 0.949 | |
423 | 419 | S | 0.67 | 1574.6 | 4.7 | 0.043 | 0 | 0.569 | |
432 | 428 | E | 0 | 2.9 | 3.1 | 0.0999 | 1 | ||
441 | 437 | Y | 1.43 | 39.3 | 4.2 | 0.0573 | 0 | 0.912 | |
478 | 474 | V | 0 | 10.3 | 6.9 | 0.0139 | 1 | 0.646 | |
502 | 498 | Y | 0.5 | 1790.4 | 4.9 | 0.0393 | 0 | 0.583 | |
508 | 504 | R | 0.519 | ||||||
528 | 524 | N | 0 | 2.2 | 3.6 | 0.0772 | 0 | ||
541 | 537 | Q | 0 | 1999.2 | 10.4 | 0.0024 | 1 | ||
542 | 538 | L | 0.56 | 68 | 6.3 | 0.0197 | 0 | ||
550 | 542 | T | 0.74 | 3332.9 | 4.3 | 0.0541 | 0 |
1 = amino acid. 2=All recovered sites in a single partition with a p+ value of 1.000.
P. pyralis ID (OGS1.1) | Predicted function | Ppyr expression rank | Ppyr BSN-TPM | Orthogroup | Alat expression rank | Alat BSN-TPM | A. lateralis ID (OGS1.0) |
---|---|---|---|---|---|---|---|
PPYR_04589 | Fatty-acid binding protein | 1 | 70912 | OG0000524 | 2 | 31943 | AQULA_005253 |
PPYR_04589 | Fatty-acid binding protein | 1 | 70912 | OG0000524 | 8 | 10464 | AQULA_005257 |
PPYR_04589 | Fatty-acid binding protein | 1 | 70912 | OG0000524 | 10 | 8520 | AQULA_005259 |
PPYR_05098 | Peroxisomal biogenesis factor 11 (PEX11) | 15 | 4005 | OG0001490 | 26 | 3294 | AQULA_005466 |
PPYR_14966 | Octopamine binding secreted hemocyanin | 34 | 2353 | OG0000369 | 21 | 3658 | AQULA_008529 |
PPYR_11733 | MFS transporter superfamily | 42 | 1853 | OG0000980 | 84 | 1335 | AQULA_012209 |
PPYR_07633 | Reticulon | 56 | 1556 | OG0004764 | 109 | 1123 | AQULA_005090 |
PPYR_09394 | lysosomal Cystine Transporter | 87 | 1098 | OG0000847 | 69 | 1494 | AQULA_009474 |
PPYR_08979 | PF03670 Uncharacterised protein family | 114 | 860 | OG0003009 | 340 | 411 | AQULA_012099 |
PPYR_05852 | Vacuolar ATP synthase 16 kDa subunit | 118 | 836 | OG0001039 | 287 | 475 | AQULA_001418 |
PPYR_11443 | RNA-binding domain superfamily | 134 | 782 | OG0004268 | 1221 | 108 | AQULA_003174 |
PPYR_02465 | Peroxin 13 | 189 | 581 | OG0001667 | 196 | 710 | AQULA_010288 |
PPYR_06160 | V-type ATPase, V0 complex | 209 | 543 | OG0000381 | 541 | 251 | AQULA_000400 |
PPYR_11300 | Mitochondrial outer membrane translocase complex | 232 | 509 | OG0004557 | 402 | 349 | AQULA_004355 |
PPYR_08174 | PF03650 Uncharacterised protein family | 249 | 475 | OG0000647 | 163 | 836 | AQULA_009867 |
PPYR_04602 | Leucine-rich repeat domain superfamily | 262 | 459 | OG0004508 | 378 | 373 | AQULA_004134 |
PPYR_01678 | MFS transporter superfamily | 264 | 458 | OG0000347 | 455 | 302 | AQULA_002485 |
PPYR_08192 | PF03650 Uncharacterised protein family | 271 | 453 | OG0000647 | 163 | 836 | AQULA_009867 |
PPYR_13497 | Mitochondrial substrate/solute carrier | 285 | 438 | OG0004402 | 379 | 372 | AQULA_003680 |
PPYR_08917 | LysM domain superfamily | 315 | 398 | OG0002035 | 483 | 278 | AQULA_002396 |
PPYR_04424 | Domain of unknown function (DUF4782) | 332 | 379 | OG0007447 | 1296 | 101 | AQULA_013946 |
PPYR_08278 | Protein of unknown function DUF1151 | 348 | 365 | OG0001306 | 430 | 325 | AQULA_000628 |
PPYR_13261 | Major facilitator superfamily | 404 | 309 | OG0000410 | 158 | 862 | AQULA_007558 |
PPYR_14848 | Homeobox-like domain superfamily - Abdominal-B-like | 413 | 304 | OG0001849 | 737 | 186 | AQULA_000483 |
PPYR_11623 | GNS1/SUR4 family | 446 | 281 | OG0008603 | 308 | 449 | AQULA_009341 |
PPYR_01828 | TLDc domain | 490 | 250 | OG0002035 | 483 | 278 | AQULA_002396 |
PPYR_03449 | Innexin | 533 | 230 | OG0000992 | 619 | 219 | AQULA_013430 |
PPYR_05702 | Sulfate permease family | 543 | 225 | OG0007205 | 396 | 357 | AQULA_013064 |
PPYR_05993 | V-type ATPase, V0 complex, 116 kDa subunit family | 579 | 210 | OG0000381 | 541 | 251 | AQULA_000400 |
PPYR_04179 | Haemolymph juvenile hormone binding protein | 606 | 202 | OG0002916 | 879 | 152 | AQULA_011187 |
PPYR_08298 | Peroxisomal membrane protein (Pex16) | 623 | 198 | OG0007339 | 395 | 358 | AQULA_013536 |
PPYR_06294 | Homeobox-like domain superfamily - Abdominal-B-like | 627 | 197 | OG0001849 | 737 | 186 | AQULA_000483 |
PPYR_05397 | PDZ superfamily | 773 | 164 | OG0006975 | 367 | 379 | AQULA_012321 |
PPYR_12625 | Homeobox domain | 796 | 160 | OG0002661 | 1395 | 95 | AQULA_008665 |
PPYR_08494 | Armadillo-type fold | 846 | 152 | OG0001600 | 986 | 133 | AQULA_008183 |
PPYR_09217 | Haemolymph juvenile hormone binding protein | 853 | 151 | OG0001089 | 441 | 316 | AQULA_003304 |
PPYR_01677 | MFS transporter superfamily | 1234 | 108 | OG0000347 | 455 | 302 | AQULA_002485 |
Retention time and m/z values are not calibrated to the other samples.
Assigned ion identity | Ion type | Chemical formula | Expected M/z | Measured M/z | M/z error* (ppm) | Retention time (mins) | Feature area (arb) |
---|---|---|---|---|---|---|---|
Core lucibufagin isomer 1 | [M + H]+ | C24H33O8 | 449.2175 | 449.2171 | −0.89 | 7.9 | 6.7E + 05 |
Core lucibufagin isomer 2 | "" | “” | “” | "" | “” | 9.3 | 1.1E + 07 |
Monoacetylated lucibufagin isomer 1 | "" | C26H35O9 | 491.2281 | 491.2277 | −0.81 | 10.2 | 4.2E + 07 |
Core lucibufagin isomer 3 | "" | C24H33O8 | 449.2175 | 449.2171 | −0.89 | 10.8 | 1.7E + 07 |
Monoacetylated lucibufagin isomer 2 | "" | C26H35O9 | 491.2281 | 491.2277 | −0.81 | 11.4 | 1.1E + 06 |
Monoacetylated lucibufagin isomer 3 | "" | “” | “” | "" | “” | 11.9 | 1.8E + 07 |
Monoacetylated lucibufagin isomer 4 | "" | “” | “” | "" | “” | 13.0 | 2.7E + 08 |
Monoacetylated lucibufagin isomer 5 | "" | “” | “” | "" | “” | 13.2 | 6.0E + 07 |
Monoacetylated lucibufagin isomer 6 | "" | “” | “” | "" | “” | 14.5 | 6.2E + 06 |
Diacetylated lucibufagin isomer 1 | "" | C28H37O10 | 533.2387 | 533.2385 | −0.37 | 15.1 | 4.0E + 09 |
Diacetylated lucibufagin isomer 2 | "" | “” | “” | "" | “” | 15.4 | 1.9E + 09 |
Monoacetylated, mono propylated lucibufagin isomer 1 | "" | C29H39O10 | 547.2543 | 547.2542 | −0.18 | 17.0 | 1.5E + 07 |
Monoacetylated, mono propylated lucibufagin isomer 2 | "" | “” | “” | "" | “” | 17.4 | 2.8E + 08 |
Monoacetylated, mono propylated lucibufagin isomer 3 | "" | “” | “” | "" | “” | 17.7 | 1.2E + 08 |
Dipropylated lucibufagin isomer 1 | "" | C30H41O10 | 561.2700 | 561.2695 | −0.89 | 18.9 | 1.4E + 08 |
Dipropylated lucibufagin isomer 2 | "" | “” | “” | "" | “” | 19.5 | 3.9E + 07 |
Dipropylated lucibufagin isomer 3 | "" | “” | “” | "" | “” | 19.8 | 1.8E + 08 |
Retention time and m/z values are not calibrated to the other samples. *=m/z error and expected m/z extrapolated from ions with similar m/z, and chemical formula predicted from resulting …
Assigned ion identity | Ion type | Chemical formula | Expected m/z | Measured m/z | m/z error (ppm) | Retention time (mins) | Feature area (arb) |
---|---|---|---|---|---|---|---|
Core lucibufagin isomer 2 | [M + H]+ | C24H33O8 | 449.2175 | 449.2215 | +8.9 | 9.15 | 8.5E + 06 |
Monoacetylated lucibufagin isomer 1 | “” | C26H35O9 | 491.2277 | 491.2326 | +9.9 | 10.04 | 1.2E + 07 |
Unknown | unknown | C28H39O10* | 535.2543* | 535.2592 | +9.1* | 12.40 | 1.6E + 07 |
Unknown | unknown | C24H38NO6* | 436.2695* | 436.2735 | +9.1* | 13.30 | 2.2E + 07 |
Unknown | unknown | C27H45N2O8* | 525.3173* | 525.3221 | +9.1* | 13.35 | 1.3E + 08 |
Unknown | unknown | C24H40NO7* | 454.2799* | 454.2840 | +9.1* | 13.73 | 1.3E + 07 |
Diacetylated lucibufagin isomer 1 | [M + H]+ | C28H37O10 | 533.2387 | 533.2426 | +7.3 | 14.93 | 1.7E + 09 |
Diacetylated lucibufagin isomer 2 | [M + H]+ | “” | “” | 533.2426 | +7.3 | 15.16 | 3.5E + 08 |
Unknown | Unknown | C29H46NO8* | 536.3216* | 536.3256 | +7.3* | 16.57 | 4.1E + 07 |
Unknown | Unknown | Unknown** | 563.2854* | 563.2896 | +7.3* | 16.80 | 1.3E + 07 |
Unknown | Unknown | C26H31O7 | 455.2056 | 455.2097 | +9.1* | 17.22 | 5.8E + 07 |
Dipropylated lucibufagin isomer 3 | Unknown | C30H41O10 | 561.2700 | 561.2738 | +6.7 | 19.53 | 2.0E + 09 |
Dipropylated lucibufagin isomer 4 | Unknown | C30H41O10 | 561.2700 | 561.2738 | +6.7 | 19.82 | 2.2E + 08 |
Used for multi-ion-chromatogram (MIC) traces in Figure 6B.
Chemical formula | Predicted exact mass | Exact mass adjusted to P. pyralis hemolymph data (+0.6 ppm) | Exact mass adjusted to P. pyralis partial larval body data (+9.9 ppm) | Exact mass adjusted to A. lateralis hemolymph data (+1.6 ppm) | Exact mass adjusted to A. lateralis larval body data (+1.1 ppm) | Exact mass adjusted to I. luminosus thorax data (+0.6 ppm) |
---|---|---|---|---|---|---|
C24H33O8 | 449.2175 | 449.2178 | 449.2219 | 449.2182 | 449.2180 | 449.2178 |
C24H38NO6* | 436.2699 | 436.2702 | 436.2742 | 436.2706 | 436.2704 | 436.2702 |
C24H40NO7* | 454.2804 | 454.2807 | 454.2849 | 454.2811 | 454.2809 | 454.2807 |
C26H31O7 | 455.2069 | 455.2072 | 455.2114 | 455.2076 | 455.2074 | 455.2072 |
C26H35O9 | 491.2281 | 491.2284 | 491.2330 | 491.2289 | 491.2286 | 491.2284 |
C27H45N2O8* | 525.3175 | 525.3178 | 525.3227 | 525.3183 | 525.3181 | 525.3178 |
C28H37O10 | 533.2386 | 533.2389 | 533.2439 | 533.2395 | 533.2392 | 533.2389 |
C28H39O10* | 535.2543 | 535.2546 | 535.2596 | 535.2552 | 535.2549 | 535.2546 |
C29H39O10 | 547.2543 | 547.2546 | 547.2597 | 547.2552 | 547.2549 | 547.2546 |
C29H46NO8* | 536.3223 | 536.3226 | 536.3276 | 536.3232 | 536.3229 | 536.3226 |
C30H41O10 | 561.2699 | 561.2702 | 561.2755 | 561.2708 | 561.2705 | 561.2702 |
*=Chemical formula assigned for structurally unclear putative lucibufagins
Assigned identity | M/z | Chemical formula | RT (mins) | Similarity score | # of ions matched | A. lateralis feature area (arb) | P. pyralis feature area (arb) |
---|---|---|---|---|---|---|---|
Unknown | 460.2462 | C22H38NO7P*; C25H29N7O2* | 15.27 | 4.10E + 11 | 34 | 7.04E + 08 | 0.00E + 00 |
"" | 657.2229 | N.D. | 12.01 | 9.50E + 11 | 29 | 6.13E + 07 | "" |
"" | 414.2043 | N.D. | 18.07 | 1.20E + 11 | 25 | 5.61E + 06 | "" |
"" | 381.2176 | C23H28N2O3* | 15.77 | 3.80E + 11 | 18 | 1.22E + 08 | "" |
"" | 476.1839 | N.D. | 15.93 | 3.80E + 11 | 16 | 9.87E + 06 | "" |
"" | 456.2148 | N.D. | 19 | 2.30E + 11 | 14 | 5.03E + 06 | "" |
"" | 351.228 | N.D. | 19.42 | 2.60E + 11 | 13 | 1.56E + 07 | "" |
"" | 479.1948 | N.D. | 19.83 | 2.20E + 11 | 12 | 1.11E + 07 | "" |
*Determined with Sirius (MS2 analysis), and MZmine2 (isotope pattern analysis).
N.D., Not determined
Genome segment | Size (nt) | Gene product (aa) | Best hit | Best hit taxonomy | Query cover | E value | Identity |
---|---|---|---|---|---|---|---|
PpyrOMLV1-PB1 | 2510 | 801 PB1 | Wuhan Mothfly Virus | Orthomyxoviridae | 83% | 0.0 | 51% |
PpyrOMLV1-PA | 2346 | 754 PA | Hubei earwig virus 1 | Orthomyxoviridae | 98% | 4.00E-137 | 35% |
PpyrOMLV1-HA | 1667 | 526 HA | Tjuloc virus | Orthomyxoviridae | 91% | 9.00E-25 | 25% |
PpyrOMLV1-PB2 | 2517 | 804 PB2 | Hubei earwig virus 1 | Orthomyxoviridae | 91% | 3.00E-118 | 31% |
PpyrOMLV1-N | 1835 | 562 N | Hubei earwig virus 1 | Orthomyxoviridae | 93% | 8.00E-74 | 30% |
PpyrOMLV2-PB1 | 2495 | 802 PB1 | Hubei orthomyxo- like virus 1 | Orthomyxoviridae | 93% | 0.0 | 48% |
PpyrOMLV2-PA | 2349 | 762 PA | Hubei earwig virus 1 | Orthomyxoviridae | 98% | 1.00E-107 | 31% |
PpyrOMLV2-HA | 1668 | 525 HA | Wellfleet Bay virus | Orthomyxoviridae | 82% | 3.00E-40 | 26% |
PpyrOMLV2-PB2 | 2506 | 801 PB2 | Hubei earwig virus 1 | Orthomyxoviridae | 96% | 3.00E-86 | 27% |
PpyrOMLV2-N | 1738 | 528 N | Hubei earwig virus 1 | Orthomyxoviridae | 95% | 6.00E-82 | 32% |
Genome product | Annotation | Start | End | Length | Database | Id | InterPro ID | InterPro name |
---|---|---|---|---|---|---|---|---|
PpyrOMLV1 -PB1 | Flu_PB1 | 48 | 752 | 705 | PFAM | PF00602 | IPR001407 | RNA_pol_PB1_ influenza |
RDRP _SSRNA | 330 | 529 | 200 | PROSITE_ PROFILES | PS50525 | IPR007099 | RNA-dir_pol_ NSvirus | |
PpyrOMLV2 -PB1 | Flu_PB1 | 54 | 766 | 713 | PFAM | PF00602 | IPR001407 | RNA_pol_PB1_ influenza |
RDRP _SSRNA | 337 | 539 | 203 | PROSITE_ PROFILES | PS50525 | IPR007099 | RNA-dir_pol_ NSvirus | |
PpyrOMLV1 -PB2 | Flu_PB2 | 13 | 421 | 409 | PFAM | PF00604 | IPR001591 | RNA_pol_PB2_ orthomyxovir |
PpyrOMLV2 -PB2 | Flu_PB2 | 13 | 415 | 403 | PFAM | PF00604 | IPR001591 | RNA_pol_PB2_ orthomyxovir |
PpyrOMLV1 -HA | SignalP-noTM | 1 | 19 | 19 | SIGNALP_ EUK | SignalP-noTM | Unintegrated | |
Baculo _gp64 | 108 | 432 | 325 | PFAM | PF03273 | IPR004955 | Baculovirus_ Gp64 | |
PpyrOMLV2 -HA | SignalP-noTM | 1 | 21 | 21 | SIGNALP_ EUK | SignalP-noTM | Unintegrated | |
Baculo _gp64 | 66 | 426 | 361 | PFAM | PF03273 | IPR004955 | Baculovirus_ Gp64 | |
PpyrOMLV1 -PA | Flu_PA | 663 | 736 | 74 | PFAM | PF00603 | IPR001009 | RNA-dir_pol_ influenzavirus |
PpyrOMLV2 -PA | Flu_PA | 667 | 740 | 74 | PFAM | PF00603 | IPR001009 | RNA-dir_pol_ influenzavirus |
PpyrOMLV1 -PB1 | flu NP-like | 94 | 459 | 366 | SUPER FAMILY | SSF161003 | Unintegrated | |
PpyrOMLV2 -PB1 | flu NP-like | 363 | 483 | 121 | SUPER FAMILY | SSF161003 | Unintegrated |
SRR 3883773 | SRR 3883772 | SRR 3883758 | SRR 3883771 | SRR 3883770 | SRR 3883769 | SRR 3883768 | SRR 3883767 | SRR 3883765 | SRR 3883764 | SRR 3883763 | SRR 3883762 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Ppyr OMLV1 HA | 11 | 541 | 2 | 160 | 0 | 4 | 881 | 2 | 0 | 2 | 199 | 2848 |
Ppyr OMLV1 NP | 0 | 321 | 0 | 141 | 0 | 0 | 523 | 0 | 0 | 0 | 120 | 1460 |
Ppyr OMLV1 PA | 3 | 256 | 0 | 95 | 0 | 0 | 306 | 1 | 0 | 5 | 100 | 660 |
Ppyr OMLV1 PB1 | 2 | 364 | 2 | 208 | 0 | 4 | 820 | 0 | 0 | 0 | 669 | 1464 |
Ppyr OMLV1 PB2 | 5 | 194 | 0 | 152 | 2 | 0 | 319 | 2 | 0 | 0 | 106 | 696 |
Ppyr OMLV2 HA | 12 | 444 | 266 | 124 | 54 | 247 | 549 | 38 | 22 | 10 | 232 | 710 |
Ppyr OMLV2 NP | 29 | 526 | 275 | 144 | 66 | 299 | 653 | 24 | 205 | 57 | 274 | 1067 |
Ppyr OMLV2 PA | 12 | 88 | 216 | 72 | 40 | 204 | 97 | 18 | 15 | 8 | 50 | 838 |
Ppyr OMLV2 PB1 | 9 | 115 | 75 | 72 | 26 | 78 | 76 | 8 | 74 | 57 | 146 | 493 |
Ppyr OMLV2 PB2 | 5 | 50 | 57 | 67 | 47 | 131 | 110 | 22 | 85 | 72 | 173 | 728 |
SRR 3883761 | SRR 3883760 | SRR 3883759 | SRR 3883757 | SRR 3883756 | SRR 3883766 | SRR 2103867 | SRR 2103849 | SRR 2103848 | Ppyr _larvae | Ppyr _Female | Ppyr _eggs | |
Ppyr OMLV1 HA | 0 | 578 | 2 | 6 | 867 | 0 | 0 | 0 | 0 | 1664 | 7826 | 15586 |
Ppyr OMLV1 NP | 0 | 289 | 0 | 3 | 647 | 0 | 2 | 0 | 0 | 644 | 5216 | 6562 |
Ppyr OMLV1 PA | 0 | 124 | 0 | 2 | 626 | 0 | 0 | 0 | 0 | 1264 | 3692 | 9564 |
Ppyr OMLV1 PB1 | 2 | 460 | 0 | 3 | 1607 | 2 | 0 | 0 | 0 | 2824 | 7144 | 15952 |
Ppyr OMLV1 PB2 | 0 | 188 | 0 | 2 | 848 | 0 | 0 | 0 | 0 | 648 | 2562 | 10568 |
Ppyr OMLV2 HA | 13 | 236 | 23 | 546 | 337 | 286 | 43 | 190 | 415 | 0 | 0 | 0 |
Ppyr OMLV2 NP | 32 | 248 | 22 | 501 | 482 | 196 | 51 | 127 | 432 | 0 | 0 | 0 |
Ppyr OMLV2 PA | 14 | 93 | 6 | 234 | 222 | 131 | 75 | 54 | 97 | 0 | 0 | 0 |
Ppyr OMLV2 PB1 | 29 | 90 | 4 | 168 | 180 | 63 | 22 | 96 | 190 | 0 | 0 | 0 |
Ppyr OMLV2 PB2 | 49 | 90 | 6 | 256 | 230 | 94 | 22 | 57 | 96 | 0 | 0 | 0 |
SRR 3883773 | SRR 3883772 | SRR 3883758 | SRR 3883771 | SRR 3883770 | SRR 3883769 | SRR 3883768 | SRR 3883767 | SRR 3883765 | SRR 3883764 | SRR 3883763 | SRR 3883762 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Ppyr OMLV1 HA | 19.10 | 0.32 | 0.05 | 6.46 | 0.00 | 0.11 | 30.69 | 0.05 | 0.00 | 0.08 | 4.07 | 69.54 |
Ppyr OMLV1 NP | 10.37 | 0.00 | 0.00 | 5.21 | 0.00 | 0.00 | 16.66 | 0.00 | 0.00 | 0.00 | 2.24 | 32.61 |
Ppyr OMLV1 PA | 6.46 | 0.06 | 0.00 | 2.74 | 0.00 | 0.00 | 7.62 | 0.02 | 0.00 | 0.13 | 1.46 | 11.52 |
Ppyr OMLV1 PB1 | 8.53 | 0.04 | 0.04 | 5.57 | 0.00 | 0.07 | 18.95 | 0.00 | 0.00 | 0.00 | 9.07 | 23.72 |
Ppyr OMLV1 PB2 | 4.50 | 0.10 | 0.00 | 4.03 | 0.05 | 0.00 | 7.29 | 0.03 | 0.00 | 0.00 | 1.42 | 11.16 |
Ppyr OMLV2 HA | 16.13 | 0.36 | 7.41 | 5.15 | 2.31 | 6.80 | 19.68 | 0.90 | 1.05 | 0.39 | 4.88 | 17.84 |
Ppyr OMLV2 NP | 17.36 | 0.79 | 6.96 | 5.44 | 2.57 | 7.48 | 21.27 | 0.52 | 8.87 | 2.01 | 5.24 | 24.36 |
Ppyr OMLV2 PA | 2.21 | 0.25 | 4.17 | 2.07 | 1.19 | 3.89 | 2.41 | 0.30 | 0.49 | 0.21 | 0.73 | 14.58 |
Ppyr OMLV2 PB1 | 2.73 | 0.18 | 1.37 | 1.95 | 0.73 | 1.40 | 1.78 | 0.12 | 2.30 | 1.44 | 2.01 | 8.10 |
Ppyr OMLV2 PB2 | 1.18 | 0.10 | 1.03 | 1.81 | 1.31 | 2.34 | 2.56 | 0.34 | 2.63 | 1.81 | 2.36 | 11.88 |
SRR 3883761 | SRR 3883760 | SRR 3883759 | SRR 3883757 | SRR 3883756 | SRR 3883766 | SRR 2103867 | SRR 2103849 | SRR 2103848 | Ppyr_ larvae | Ppyr_ Female | Ppyr_ eggs | |
Ppyr OMLV1 HA | 0.00 | 18.29 | 0.08 | 0.21 | 23.44 | 0.00 | 0.00 | 0.00 | 0.00 | 15.89 | 74.25 | 104.49 |
Ppyr OMLV1 NP | 0.00 | 8.37 | 0.00 | 0.09 | 16.00 | 0.00 | 0.04 | 0.00 | 0.00 | 5.62 | 45.27 | 40.24 |
Ppyr OMLV1 PA | 0.00 | 2.81 | 0.00 | 0.05 | 12.10 | 0.00 | 0.00 | 0.00 | 0.00 | 8.63 | 25.05 | 45.85 |
Ppyr OMLV1 PB1 | 0.04 | 9.66 | 0.00 | 0.07 | 28.83 | 0.04 | 0.00 | 0.00 | 0.00 | 17.89 | 44.97 | 70.96 |
Ppyr OMLV1 PB2 | 0.00 | 3.91 | 0.00 | 0.05 | 15.05 | 0.00 | 0.00 | 0.00 | 0.00 | 4.06 | 15.96 | 46.51 |
Ppyr OMLV2 HA | 0.43 | 7.68 | 0.95 | 19.30 | 9.38 | 9.74 | 1.02 | 4.94 | 8.95 | 0.00 | 0.00 | 0.00 |
Ppyr OMLV2 NP | 0.97 | 7.34 | 0.82 | 16.09 | 12.19 | 6.07 | 1.10 | 3.00 | 8.47 | 0.00 | 0.00 | 0.00 |
Ppyr OMLV2 PA | 0.32 | 2.10 | 0.17 | 5.73 | 4.28 | 3.09 | 1.23 | 0.97 | 1.45 | 0.00 | 0.00 | 0.00 |
Ppyr OMLV2 PB1 | 0.63 | 1.92 | 0.11 | 3.88 | 3.27 | 1.40 | 0.34 | 1.63 | 2.68 | 0.00 | 0.00 | 0.00 |
Ppyr OMLV2 PB2 | 1.06 | 1.90 | 0.16 | 5.88 | 4.16 | 2.08 | 0.34 | 0.96 | 1.35 | 0.00 | 0.00 | 0.00 |
Scaffold | Start | End | Strand | Id with PpOMLV | E value | Coverage | FEVE |
---|---|---|---|---|---|---|---|
Ppyr1.2_LG1 | 12787323 | 12786796 | (-) | 56.30% | 8.22E-50 | 39.10% | EVE PB1 like-1 |
Ppyr1.2_LG1 | 13016647 | 13016120 | (-) | 56.30% | 8.22E-50 | 39.10% | EVE PB1 like-2 |
Ppyr1.2_LG1 | 34701480 | 34701560 | (+) | 37.00% | 2.88E-26 | 26.70% | EVE PB1 like-3 |
Ppyr1.2_LG1 | 34701562 | 34701774 | (+) | 37.60% | 2.88E-26 | 30.20% | EVE PB1 like-3 |
Ppyr1.2_LG1 | 34701801 | 34702214 | (+) | 45.30% | 2.88E-26 | 34.00% | EVE PB1 like-3 |
Ppyr1.2_LG1 | 35094645 | 35095094 | (+) | 28.10% | 2.15E-10 | 9.50% | EVE PB1 like-4 |
Ppyr1.2_LG1 | 35110084 | 35109956 | (-) | 53.50% | 2.37E-14 | 4.40% | EVE PB1 like-5 |
Ppyr1.2_LG1 | 35110214 | 35110107 | (-) | 75.00% | 2.37E-14 | 14.70% | EVE PB1 like-5 |
Ppyr1.2_LG1 | 35110347 | 35110213 | (-) | 42.60% | 2.37E-14 | 2.90% | EVE PB1 like-5 |
Ppyr1.2_LG1 | 50031464 | 50031330 | (-) | 64.40% | 1.18E-09 | 10.00% | EVE PB1 like-6 |
Ppyr1.2_LG1 | 50031498 | 50031457 | (-) | 71.40% | 1.18E-09 | 11.60% | EVE PB1 like-6 |
Ppyr1.2_LG1 | 50613130 | 50612921 | (+) | 49.40% | 3.71E-11 | 4.90% | EVE PB1 like-7 |
Ppyr1.2_LG1 | 50673211 | 50673621 | (+) | 38.50% | 1.03E-12 | 9.70% | EVE PB1 like-8 |
Ppyr1.2_LG1 | 51208464 | 51207634 | (-) | 77.20% | 0 | 56.40% | EVE PB1 like-9 |
Ppyr1.2_LG1 | 51209399 | 51208467 | (-) | 68.50% | 0 | 53.60% | EVE PB1 like-9 |
Ppyr1.2_LG1 | 51209556 | 51209398 | (-) | 71.70% | 0 | 39.20% | EVE PB1 like-9 |
Ppyr1.2_LG1 | 61871682 | 61872158 | (+) | 31.10% | 2.84E-23 | 36.00% | EVE PB1 like-10 |
Ppyr1.2_LG1 | 61872158 | 61872319 | (+) | 46.30% | 2.84E-23 | 28.30% | EVE PB1 like-10 |
Ppyr1.2_LG1 | 61872355 | 61872456 | (+) | 41.20% | 2.84E-23 | 27.00% | EVE PB1 like-10 |
Ppyr1.2_LG1 | 61930528 | 61930205 | (-) | 38.00% | 3.58E-27 | 30.90% | EVE PB1 like-11 |
Ppyr1.2_LG1 | 61930686 | 61930504 | (-) | 63.60% | 3.58E-27 | 35.90% | EVE PB1 like-11 |
Ppyr1.2_LG1 | 68038999 | 68039073 | (+) | 60.00% | 7.73E-12 | 6.60% | EVE PB1 like-12 |
Ppyr1.2_LG1 | 68039072 | 68039314 | (+) | 40.70% | 7.73E-12 | 5.00% | EVE PB1 like-12 |
Ppyr1.2_LG1 | 68039289 | 68039330 | (+) | 64.30% | 7.73E-12 | 8.00% | EVE PB1 like-12 |
Ppyr1.2_LG1 | 68128820 | 68129008 | (+) | 51.50% | 1.89E-06 | 4.90% | EVE PB1 like-13 |
Ppyr1.2_LG2 | 34545814 | 34545680 | (-) | 58.70% | 3.84E-06 | 7.20% | EVE PB1 like-14 |
Ppyr1.2_LG2 | 34546169 | 34545801 | (-) | 52.80% | 1.16E-31 | 34.10% | EVE PB1 like-14 |
Scaffold | Start | End | Strand | Id with PpOMLV | E value | Coverage | FEVE |
---|---|---|---|---|---|---|---|
Ppyr1.2_LG1 | 50313869 | 50314219 | (+) | 82.10% | 6.91E-54 | 48.30% | EVE PB2 like-1 |
Ppyr1.2_LG1 | 50314216 | 50315016 | (+) | 82.40% | 1.92E-142 | 57.90% | EVE PB2 like-1 |
Ppyr1.2_LG1 | 50315772 | 50315002 | (-) | 89.10% | 9.97E-145 | 60.60% | EVE PB2 like-1 |
Ppyr1.2_LG1 | 58707403 | 58706942 | (-) | 52.60% | 6.19E-42 | 35.80% | EVE PB2 like-2 |
Scaffold | Start | End | Strand | Id with PpOMLV | E value | Coverage | FEVE |
---|---|---|---|---|---|---|---|
Ppyr1.2_LG1 | 34977392 | 34977231 | (-) | 48.10% | 7.73E-07 | 3.50% | EVE PA like-1 |
Ppyr1.2_LG1 | 62052289 | 62052023 | (-) | 28.70% | 8.92E-11 | 7.10% | EVE PA like-2 |
Ppyr1.2_LG1 | 62117077 | 62116811 | (-) | 28.70% | 1.22E-10 | 7.10% | EVE PA like-3 |
Ppyr1.2_LG1 | 62117493 | 62117101 | (-) | 26.30% | 1.22E-10 | 8.60% | EVE PA like-3 |
Ppyr1.2_LG1 | 68122348 | 68122440 | (+) | 77.40% | 3.40E-06 | 15.70% | EVE PA like-4 |
Scaffold | Start | End | Strand | Id with PpOMLV | E value | Coverage | FEVE |
---|---|---|---|---|---|---|---|
Ppyr1.2_LG1 | 181303 | 181404 | (+) | 79.40% | 7.01E-09 | 17.90% | EVE NP like-1 |
Ppyr1.2_LG1 | 1029425 | 1029568 | (+) | 93.80% | 9.59E-21 | 27.40% | EVE NP like-2 |
Ppyr1.2_LG1 | 2027860 | 2027438 | (-) | 35.50% | 3.00E-21 | 30.80% | EVE NP like-3 |
Ppyr1.2_LG1 | 36568324 | 36568551 | (+) | 42.10% | 8.99E-11 | 7.20% | EVE NP like-4 |
Ppyr1.2_LG1 | 52877256 | 52877086 | (-) | 68.40% | 3.87E-15 | 14.60% | EVE NP like-5 |
Ppyr1.2_LG1 | 59927414 | 59927271 | (+) | 93.80% | 5.60E-20 | 26.40% | EVE NP like-6 |
Ppyr1.2_LG3 | 17204346 | 17204122 | (-) | 46.70% | 7.60E-13 | 7.10% | EVE NP like-7 |
Ppyr1.2_LG3 | 31635344 | 31635030 | (-) | 35.80% | 3.30E-08 | 10.00% | EVE NP like-8 |
Ppyr1.2_LG3 | 50175821 | 50175922 | (+) | 79.40% | 7.01E-09 | 17.90% | EVE NP like-9 |
Ppyr1.2_LG4 | 27811681 | 27811758 | (+) | 38.50% | 3.22E-13 | 2.50% | EVE NP like-10 |
Ppyr1.2_LG4 | 27811853 | 27812179 | (+) | 39.00% | 3.22E-13 | 10.90% | EVE NP like-10 |