H358 cells were treated for 10 min with TYPE7 (2 μM) and the following controls: Fc (CT), EA1 (0.5 μg/mL) and pHLIP (2 μM). After treatment, cell lysates were incubated overnight with array membranes (R and D Systems ARY003B) for duplicated detection of phosphorylation of 43 total kinases (A) and their substrates (B). Myristoylated Src family kinases are boxed: top (Hck, Fyn and Src), middle (Yes and Lyn), and bottom (Lck). The pHLIP peptide was used as a control for specificity. The array contains the following proteins, in order from top to bottom, and then left to right: p38α, ERK1/2, JNK 1/2/3, GSK-3α/β, p53, EGFR, MSK1/2, AMPKα1, Akt, p53, TOR, CREB, HSP27, AMPKα2, β-Catenin, p70 S6 Kinase, p53, c-Jun, Src, Lyn, Lck, STAT2, STAT5a, p70 S6 Kinase, RSK1/2/3, eNOS, Fyn, Yes, Fgr, STAT6, STAT5b, STAT3, p27, PLC-g1, Hck, Chk-2, FAX, PDGFRb, STAT5a/b, STAT3, WNK1, PYK2, PRAS40 and HSP60. (C) Quantification of Akt phosphorylation (p–S473).