Cryo-EM structure of the polycystin 2-l1 ion channel
Abstract
We report the near atomic resolution (3.3 Å) of the human polycystic kidney disease 2-like 1 (polycystin 2-l1) ion channel. Encoded by PKD2L1, polycystin 2-l1 is a calcium and monovalent cation-permeant ion channel in primary cilia and plasma membranes. The related primary cilium-specific polycystin-2 protein, encoded by PKD2, shares a high degree of sequence similarity, yet has distinct permeability characteristics. Here we show that these differences are reflected in the architecture of polycystin 2-l1.
Data availability
The cryo map and model have been deposited to the Worldwide Protein Data Bank (6DU8) and Electron Microscopy Data Bank (8912).
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Cryo map and model fromPublicly available at the Worldwide Protein Data Bank (Accession no: 6DU8).
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Cryo map and model fromPublicly available at the Electron Microscopy Data Bank (Accession no: 8912).
Article and author information
Author details
Funding
Howard Hughes Medical Institute
- David E Clapham
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Baron Chanda, University of Wisconsin-Madison, United States
Version history
- Received: March 23, 2018
- Accepted: July 12, 2018
- Accepted Manuscript published: July 13, 2018 (version 1)
- Version of Record published: July 23, 2018 (version 2)
Copyright
© 2018, Hulse et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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