(A) Schematic for strategy to examine the role of the EMC (Emc5-BirA) in cotranslational interaction with clients using proximity-specific ribosome profiling. (B) Positional enrichment plots showing footprint reads across the full-length mRNAs of the genes indicated for Emc5-BirA, BirA-Ubc6-TA and Ssh1-BirA expressing strains. Transmembrane domains (TMDs) are indicated in gray. (C) As in (B), except comparing Emc5-BirA and Sec63-BirA expressing strains. (D) Mean enrichments of all TMD-containing genes and EMC clients (N = 51) following start of first TMD for two independent replicates of Emc5-BirA, Sec63-BirA, BirA-Ssh1 and BirA-Ubc6-TA.