(A) Schematic of MAPLE with workspace dimensions indicated and an expanded schematic of the end effectors. Colors indicate different robotic systems. (B–F) Demonstrations of MAPLE experiments in yeast, C. elegans, Physarum polycephalum and fruit flies. (B1) Yeast colonies were automatically picked and transferred to fresh media plates in a pattern (B2), and then streaked to grow colonies from single cells (B2, B3). B3 is an image of GFP fluorescence in the colonies demarcated by the inset box of B2. (C1) Worm locomotion on standard culture plates with OP50 bacterial lawns was captured at 1 Hz using MAPLE’s camera effector. (C2) Cropped view of one such image showing individual worms. (C3) Tracks of multiple worm motion across a plate over 8.5 min, showing the field of view MAPLE’s camera and potential for motion phenotyping. (D1) Time-lapse movies of the movement of Physarum plasmodia were recorded in parallel for nine cultures at imaging rates of 0.017 Hz (one image/min) for 12 hr. (D2) Composite of plasmodia morphologies approximately 9.6 hr into the 12 hr recording. (D3) Time-coded image of the growth of a single plasmodium, overlaying images of the plasmodium over time in colors cycling over hues. (E1) Representative MAPLE procedure for moving fruit flies between a growth/storage module (left) and a phenotyping module (right) using the organism manipulator. (E2) Photo of flies in a FlyPlate single-housing storage module. See description below. (E3) Photo of flies in a social arena phenotyping module. See description below. (F) Bee Experimental Ethology Colony Hardware (BEECH): a MAPLE-derived robotic platform for imaging bumblebee behavior. BEECH was used to record bumblebee behavior in multiple colonies over long periods of time (up to 7 days). The end effectors of this robot were IR-sensitive cameras (right) for recording digital video of bees behaving. Bees were housed in acrylic colony boxes (G1) with a dark nest compartment and circadian-lit foraging compartment where they, respectively, reared developing young and collected nectar and pollen from feeders. (G2) Image acquired from the BEECH IR camera showing automatically tracked bees in the nest chamber. Inset box expanded in (G3) to illustrate workers (red ellipses), the queen (magenta ellipse), and nest structure (yellow overlay), all of which are tracked in BEECH data sets. This figure is also a target for an augmented reality view of MAPLE. Print the figure in portrait orientation on letter paper, place the printout on a horizontal surface like a bench or desk, and view it in scan mode in the ‘Augment’ mobile app to interact with a to-scale rendering of MAPLE.