(a) At day 35, immunostaining of TUJ-1 showed a similar efficiency of neuronal differentiation in all LHX6 OE groups and controls and a higher percentage of GABA+ cells in LHX6 OE cells. Scale bar, 50 μm. (b) The GIN subtypes calbindin (CB, day 35), calretinin (CR, day 45), somatostatin (SST, day 50), nNOS (day 80) were presented in the LHX6OE cells from two cell lines. Scale bar, 50 μm. (c) Quantification of CB+, CR+, SST+, and PV+ cells among the TUJ-1+ cells. Upper, H9-01 LHX6 OE; below, ihtc-03 LHX6 OE group. (d) SST and PV expression in the ihtc-03 LHX6 OE group. (e) Neurons expressing SST and PV interneurons showed a characteristic morphological structure with more than five secondary branches.