Abstract
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise estimation of chronological age in murine blood samples, too. DBA/2 mice revealed accelerated epigenetic aging as compared to C57BL6 mice, which is in line with their shorter life-expectancy. The three-CpG-predictor provides a simple and cost-effective biomarker to determine biological age in large intervention studies with mice.
Data availability
Raw data of pyrosequencing is provided as supplemental EXCEL table (Source data 1).
Article and author information
Author details
Funding
Else Kröner-Fresenius-Stiftung (2014_A193)
- Wolfgang Wagner
Deutsche Forschungsgemeinschaft (SFBs 1275)
- Hartmut Geiger
NIH Clinical Center (R01DK104814)
- Hartmut Geiger
Bundesministerium für Bildung und Forschung (SyStarR)
- Hartmut Geiger
Deutsche Forschungsgemeinschaft (DFG; WA 1706/8-1)
- Wolfgang Wagner
Bundesministerium für Bildung und Forschung (BMBF; 01KU1402B)
- Wolfgang Wagner
NIH Clinical Center (R01HL134617)
- Hartmut Geiger
Netherland Organization for Scientific Research
- Gerald de Haan
Deutsche Forschungsgemeinschaft (GRK 1789 CEMMA)
- Hartmut Geiger
Deutsche Forschungsgemeinschaft (GRK 2254 HEIST)
- Hartmut Geiger
Deutsche Forschungsgemeinschaft (SFBs 1074)
- Hartmut Geiger
Deutsche Forschungsgemeinschaft (SFBs 1149)
- Hartmut Geiger
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Experiments were approved by the Institutional Animal Care of the Ulm University as well as by Regierungspräsidium Tübingen and by the Institutional Animal Care and Use Committee of the University of Groningen (IACUC-RUG), respectively....To analyze age-associated changes in different tissues we used 3 young (67 days old) and 3 old (398 days old) C57BL/6J mice (JaxMice) in accordance with relevant Spanish and European guidelines after approval by the Biodonostia Animal Care Committee.
Reviewing Editor
- Vadim N Gladyshev, Brigham and Women's Hospital, Harvard Medical School, United States
Publication history
- Received: April 13, 2018
- Accepted: August 23, 2018
- Accepted Manuscript published: August 24, 2018 (version 1)
- Version of Record published: September 25, 2018 (version 2)
Copyright
© 2018, Han et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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