PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN
- The Hospital for Sick Children, Canada
- University Health Network, Canada
Abstract
Centrosome structure, function, and number are finely regulated at the cellular level to ensure normal mammalian development. Here, we characterize PPP1R35 as a novel bona fide centrosomal protein and demonstrate that it is critical for centriole elongation. Using quantitative super-resolution microscopy mapping and live-cell imaging we show that PPP1R35 is a resident centrosomal protein located in the proximal lumen above the cartwheel, a region of the centriole that has eluded detailed characterization. Loss of PPP1R35 function results in decreased centrosome number and shortened centrioles that lack centriolar distal and microtubule wall associated proteins required for centriole elongation. We further demonstrate that PPP1R35 acts downstream of, and forms a complex with, RTTN, a microcephaly protein required for distal centriole elongation. Altogether, our study identifies a novel step in the centriole elongation pathway centered on PPP1R35 and elucidates downstream partners of the microcephaly protein RTTN.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Andrew Michael Sydor
Funding
National Science and Engineering Research Council of Canada (Discovery grant RGPIN-2015-04795)
- Vito Mennella
The Hospital for Sick Children (Restracomp Postdoctoral Fellowship)
- Andrew Michael Sydor
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Sydor et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN
eLife 7:e37846.
https://doi.org/10.7554/eLife.37846
