Under high CO2 conditions, no CCM is established (A). After exposure to ambient CO2, CCM is induced (B). As most of Rubisco and the other CBC enzymes are in the stroma under high CO2, most CBC flux …
Chlamydomonas reinhardtii CC1690 cells were grown under high CO2 (HC for proteins and metabolites; white bars), ambient CO2 (LC for proteins; grey bars) and ambient CO2 bubbled for 15 min with high …
Chlamydomonas reinhardtii CC1690 were grown at 46 μmol photons*m−2*s−1, 24°C and bubbled with 5% CO2 (HC) for two days at constant turbidity in a bioreactor. CO2 in the outlet air of the bioreactor …
Chlamydomonas reinhardtii CC-4533 cells expressing Venus-fusion constructs (green) were grown under ambient CO2, imaged by fluorescence microscopy and two pictures per constructs are shown. On the …
The model includes a copy of the Calvin-Benson cycle (CBC) in the chloroplast stroma and in the pyrenoid. In addition, the model considers reversible transport between the stroma and the pyrenoid …
Model predictions under non-CCM (HC, (A) and CCM-induced (LC*; B) conditions were validated using the Chi-square statistic between predicted and measured total concentration for 11 metabolites over …
(A + B) Modelled data is shown as white bars (n = 1,000 ± SD) and the SD-values are too small to be seen. Experimentally, metabolites were measured by HPLC-MS/MS as already shown in Figure 2 (n = 4 ±…
The estimated was negative for all enzymes except RPE and RPI for which . In addition, TRK was found to be the only enzyme operating in both directions.
(A) Fold changes of model predicted average net flux (represented by arrows) and total metabolite concentrations (represented by circles) between LC* and HC cells indicated by a colour code (see …
Summary of the most important concentrations and fluxes.
The average values of the flux and total metabolite concentration are indicated by a colour code (see legend) and size of the arrows (denoting the reactions). The actual values are provided in Supple…
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Chlamydomonas reinhardtii) | CC1690 wild-type strain | Chlamydomonas Resource Center | RRID:SCR_014960 | |
Strain, strain background (Chlamydomonas reinhardtii) | CC-4533 wild-type strain | Chlamydomonas Resource Center | RRID:SCR_014960 | - |
Antibody | rabbit Anti- Beta-CA1 | AgriSera | Cat# AS11 1737; RRID:AB_10752086 | (1:7500) |
Antibody | rabbit Anti-AtpD | AgriSera | Cat# AS10 1590; RRID:AB_10754669 | (1:30000) |
Software, algorithm | MaxQuant | MaxQuant (http://www.biochem.mpgde/5111795/maxquant) | RRID:SCR_014485 | version 1.5.2.8 |
Software, algorithm | Codes used for modelling | The mathematical models can be accessed via https://github.com/ankueken/Chlamy_model (copy archived at https://github.com/elifesciences-publications/Chlamy_model). |
Experimental data.
Protein information for Venus localization.
Model structure.
Predications of kcat-values compared to literature values.
Raw data of the model for HC conditions.
Raw data of the model for LC* conditions.